Restriction map for
pFN21AE0795
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 891 GAATTC [GA]^AATT[CT]
AhdI 640 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 891 GAATTC [GA]^AATT[CT]
AspEI 640 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 745 GAGCAC G[AT]GC[AT]^C
AvaI 277 CCCGAG C^[CT]CG[GA]G
BanI 24 GGCGCC G^G[CT][GA]CC
BbeI 24 GGCGCC GGCGC^C
BbvCI 562 GCTGAGG CCTCAGC(-5/-2)
BfaI 961 CTAG C^TAG
BsaWI 536 TCCGGT [AT]^CCGG[AT]
BsiHKAI 745 GAGCAC G[AT]GC[AT]^C
BsmBI 280 GAGACG CGTCTC(1/5)
BsoBI 277 CCCGAG C^[CT]CG[GA]G
BssHII 333 GCGCGC G^CGCGC
BstXI 677 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 859 AGATCC [GA]^GATC[CT]
DsaI 983 CCATGG C^C[GA][CT]GG
Eam1105I 640 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1060 CTCTTC CTCTTC(1/4)
EcoRI 891 GAATTC G^AATTC
HincII 271 GTCGAC GT[CT]^[GA]AC
HindII 271 GTCGAC GT[CT]^[GA]AC
KasI 24 GGCGCC G^GCGCC
Ksp632I 1060 CTCTTC CTCTTC(1/4)
MaeI 961 CTAG C^TAG
MaeIII 872 GTGAC ^GT[ACGT]AC
MflI 859 AGATCC [GA]^GATC[CT]
MslI 748 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 24 GGCGCC GG^CGCC
NcoI 983 CCATGG C^CATGG
NruI 411 TCGCGA TCG^CGA
PflMI 216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 295 CTGCAG CTGCA^G
PvuII 292 CAGCTG CAG^CTG
SalI 271 GTCGAC G^TCGAC
SfaNI 233 GCATC GCATC(5/9)
SfcI 295 CTGCAG C^T[GA][CT]AG
SfoI 24 GGCGCC GGC^GCC
TaqI 272 TCGA T^CGA
Tsp45I 872 GTGAC ^GT[GC]AC
Tsp509I 892 AATT ^AATT
TthHB8I 272 TCGA T^CGA
Van91I 216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 859 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 271 GTCGAC GT^[AC][GT]AC
936 GTCTAC
AcyI 24 GGCGCC G[GA]^CG[CT]C
307 GGCGTC
AfaI 111 GTAC GT^AC
464 GTAC
BalI 980 TGGCCA TGG^CCA
986 TGGCCA
BsaHI 24 GGCGCC G[GA]^CG[CT]C
307 GGCGTC
BsaI 208 GAGACC GGTCTC(1/5)
673 GAGACC
DdeI 563 CTGAG C^T[ACGT]AG
740 CTCAG
EcoNI 48 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
816 CCTGGCAGAGG
EcoT14I 830 CCTTGG C^C[AT][AT]GG
983 CCATGG
HgaI 10 GACGC GACGC(5/10)
308 GCGTC
Hin1I 24 GGCGCC G[GA]^CG[CT]C
307 GGCGTC
HinfI 553 GACTC G^A[ACGT]TC
919 GACTC
MaeII 197 ACGT A^CGT
641 ACGT
MluNI 980 TGGCCA TGG^CCA
986 TGGCCA
MscI 980 TGGCCA TGG^CCA
986 TGGCCA
MspA1I 14 CAGCGG C[AC]G^C[GT]G
292 CAGCTG
NaeI 138 GCCGGC GCC^GGC
366 GCCGGC
NgoMIV 138 GCCGGC G^CCGGC
366 GCCGGC
PleI 553 GACTC GAGTC(4/5)
919 GACTC
RsaI 111 GTAC GT^AC
464 GTAC
StyI 830 CCTTGG C^C[AT][AT]GG
983 CCATGG
XcmI 677 CCAAGCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
842 CCAGGCCAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI TspRI