(Circular) (Six-base) MAPSORT of: pFN21AE0795.seq Check: 3339 from: 1 to: 5779 pFN21AE0795 5779 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2094 2215 2094 Size: 121 5658 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 2942 3122 1937 Size: 340 665 180 4594 Fragments arranged by size: 4594 665 340 180 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2559 3052 3842 5226 5494 1632 Size: 927 493 790 1384 268 1917 Fragments arranged by size: 1917 1384 927 790 493 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 2650 4508 2650 Size: 1858 3921 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 3522 5094 3522 Size: 1572 4207 ApaI G_GGCC'C Cuts at: 2486 2590 3007 3034 2486 Size: 104 417 27 5231 Fragments arranged by size: 5231 417 104 27 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 2896 3088 3314 3466 5575 1493 Size: 1403 192 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2282 3104 1870 Size: 73 339 822 4545 Fragments arranged by size: 4545 822 339 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3100 4555 618 Size: 325 217 627 242 1071 1455 1842 Fragments arranged by size: 1842 1455 1071 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 2136 2486 2583 2590 2846 3007 3034 3098 Size: 745 350 97 7 256 161 27 64 Cuts at: 3098 1391 Size: 4072 Fragments arranged by size: 4072 745 350 256 161 97 64 27 7 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 5779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4808 BbvCI CC'TCA_GC Cuts at: 2568 2568 Size: 5779 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3970 4004 1903 Size: 34 2033 34 3678 Fragments arranged by size: 3678 2033 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 2490 2663 Size: 107 122 71 631 605 481 337 173 Cuts at: 2663 4390 136 Size: 1727 1525 Fragments arranged by size: 1727 1525 631 605 481 337 173 122 107 71 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2486 2590 2778 3007 3034 3840 4996 1790 Size: 696 104 188 229 27 806 1156 2573 Fragments arranged by size: 2573 1156 806 696 229 188 104 27 BmgBI CAC'GTC Cuts at: 1318 2647 1318 Size: 1329 4450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 3510 4468 Size: 416 53 83 399 24 1064 1029 958 Cuts at: 4468 442 Size: 1753 Fragments arranged by size: 1753 1064 1029 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2857 3067 4439 1239 Size: 836 782 210 1372 2579 Fragments arranged by size: 2579 1372 836 782 210 Bpu10I CC'TnA_GC Cuts at: 2568 2568 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 2672 4442 882 Size: 617 708 465 1770 2219 Fragments arranged by size: 2219 1770 708 617 465 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2313 3586 3968 Size: 53 83 186 936 497 283 1273 382 Cuts at: 3968 4603 275 Size: 635 1451 Fragments arranged by size: 1451 1273 936 635 497 382 283 186 83 53 BsaWI w'CCGG_w Cuts at: 2541 4212 4610 4884 5031 5442 2541 Size: 1671 398 274 147 411 2878 Fragments arranged by size: 2878 1671 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 4384 Fragments arranged by size: 4384 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2903 2938 2941 1186 Size: 1156 561 35 3 4024 Fragments arranged by size: 4024 1156 561 35 3 BseYI C'CCAG_C Cuts at: 4982 5478 4982 Size: 496 5283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 2408 2492 1163 Size: 573 336 336 84 4450 Fragments arranged by size: 4450 573 336 336 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2148 2376 3229 3990 5018 Size: 275 186 24 123 228 853 761 1028 Cuts at: 5018 1540 Size: 2301 Fragments arranged by size: 2301 1028 853 761 275 228 186 123 24 BsiHKAI G_wGCw'C Cuts at: 2754 3840 3925 4996 2754 Size: 1086 85 1071 3537 Fragments arranged by size: 3537 1086 1071 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 BsmBI CGTCTCn'nnnn_ Cuts at: 2279 2279 Size: 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2486 2583 2590 2754 2778 2846 Size: 399 346 350 97 7 164 24 68 Cuts at: 2846 3007 3034 3098 3840 3925 4996 1391 Size: 161 27 64 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 346 164 161 97 85 68 64 27 24 7 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2215 2514 3134 844 Size: 1250 121 299 620 3489 Fragments arranged by size: 3489 1250 620 299 121 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2143 2175 2371 4423 4610 1700 Size: 110 333 32 196 2052 187 2869 Fragments arranged by size: 2869 2052 333 196 187 110 32 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 2338 1925 Size: 413 5366 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5779 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2689 Size: 5779 BstYI r'GATC_y Cuts at: 1972 2864 3109 3476 3861 3878 5330 5416 5428 Size: 892 245 367 385 17 1452 86 12 Cuts at: 5428 5774 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 892 385 367 346 245 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2988 513 Size: 552 1923 3304 Fragments arranged by size: 3304 1923 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 3295 4089 4109 3295 Size: 794 20 4965 Fragments arranged by size: 4965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2145 2373 Size: 54 1425 50 275 18 192 123 228 Cuts at: 2373 2985 2991 3226 4115 5744 8 Size: 612 6 235 889 1629 43 Fragments arranged by size: 1629 1425 889 612 275 235 228 192 123 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2022 2145 2373 3226 1537 Size: 275 210 123 228 853 4090 Fragments arranged by size: 4090 853 275 228 210 123 EarI CTCTTCn'nnn_ Cuts at: 1353 3071 3709 1353 Size: 1718 638 3423 Fragments arranged by size: 3423 1718 638 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2644 2857 4366 4740 4886 387 Size: 1064 138 1055 213 1509 374 146 1280 Fragments arranged by size: 1509 1280 1064 1055 374 213 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2559 2857 3052 3067 3842 4439 Size: 393 443 484 298 195 15 775 597 Cuts at: 4439 5226 5494 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 775 597 484 443 393 298 268 195 15 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2825 2057 Size: 768 5011 EcoO109I rG'GnC_Cy Cuts at: 2482 2483 3003 3031 2482 Size: 1 520 28 5230 Fragments arranged by size: 5230 520 28 1 EcoRI G'AATT_C Cuts at: 2896 3088 3466 2896 Size: 192 378 5209 Fragments arranged by size: 5209 378 192 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2033 2354 2936 4926 1678 Size: 355 321 582 1990 2531 Fragments arranged by size: 2531 1990 582 355 321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 2636 2668 2728 Size: 32 135 32 971 32 61 32 60 Cuts at: 2728 2760 4420 4452 4494 4526 1373 Size: 32 1660 32 42 32 2626 Fragments arranged by size: 2626 1660 971 135 61 60 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2278 3123 3369 669 Size: 988 281 340 845 246 3079 Fragments arranged by size: 3079 988 845 340 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KasI G'GCGC_C Cuts at: 2029 2029 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 4893 5077 1305 Size: 1674 1914 184 2007 Fragments arranged by size: 2007 1914 1674 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 2993 10 Size: 54 1425 343 1155 6 2796 Fragments arranged by size: 2796 1425 1155 343 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 2757 3737 4096 4255 518 Size: 648 1591 980 359 159 2042 Fragments arranged by size: 2042 1591 980 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2021 2299 3195 3872 5020 5265 1606 Size: 415 278 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 415 278 245 NaeI GCC'GGC Cuts at: 2145 2373 2145 Size: 228 5551 NarI GG'CG_CC Cuts at: 2030 2030 Size: 5779 NcoI C'CATG_G Cuts at: 513 1065 2988 513 Size: 552 1923 3304 Fragments arranged by size: 3304 1923 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2143 2371 2143 Size: 228 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NruI TCG'CGA Cuts at: 2418 2418 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 3137 4682 1132 Size: 97 1908 1545 2229 Fragments arranged by size: 2229 1908 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 1108 Size: 1119 4660 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2482 2586 3003 3030 2482 Size: 104 417 27 5231 Fragments arranged by size: 5231 417 104 27 PstI C_TGCA'G Cuts at: 830 2304 3131 830 Size: 1474 827 3478 Fragments arranged by size: 3478 1474 827 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5779 SalI G'TCGA_C Cuts at: 1936 2276 3121 1936 Size: 340 845 4594 Fragments arranged by size: 4594 845 340 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2300 3127 4262 4943 5134 826 Size: 219 1255 827 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 827 681 219 191 SfoI GGC'GCC Cuts at: 2031 2031 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2835 2988 3218 513 Size: 552 1770 153 230 3074 Fragments arranged by size: 3074 1770 552 230 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3806 3823 3976 4161 4606 325 Size: 1255 2226 17 153 185 445 1498 Fragments arranged by size: 2226 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 4025 5737 Size: 274 80 33 51 1443 138 1910 1712 Cuts at: 5737 96 Size: 138 Fragments arranged by size: 1910 1712 1443 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5739 5771 5739 Size: 32 5747 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2689 2854 2689 Size: 165 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI