Restriction map for
pFN21AE0795
(Linear) (Six-base) MAPSORT of: KIEE0795.seq Check: 9884 from: 1 to: 1074
KIEE0795.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 89 210 1074
Size: 89 121 864
Fragments arranged by size:
864 121 89
AccI GT'mk_AC
Cuts at: 0 272 937 1074
Size: 272 665 137
Fragments arranged by size:
665 272 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1047 1074
Size: 554 493 27
Fragments arranged by size:
554 493 27
AhdI GACnn_n'nnGTC
Cuts at: 0 645 1074
Size: 645 429
ApaI G_GGCC'C
Cuts at: 0 481 585 1002 1029 1074
Size: 481 104 417 27 45
Fragments arranged by size:
481 417 104 45 27
ApoI r'AATT_y
Cuts at: 0 891 1074
Size: 891 183
AvaI C'yCGr_G
Cuts at: 0 277 1074
Size: 277 797
BanI G'GyrC_C
Cuts at: 0 24 1074
Size: 24 1050
BanII G_rGCy'C
Cuts at: 0 131 481 578 585 841 1002 1029 1074
Size: 131 350 97 7 256 161 27 45
Fragments arranged by size:
350 256 161 131 97 45 27 7
BbeI G_GCGC'C
Cuts at: 0 28 1074
Size: 28 1046
BbvCI CC'TCA_GC
Cuts at: 0 563 1074
Size: 563 511
BglI GCCn_nnn'nGGC
Cuts at: 0 148 485 658 1074
Size: 148 337 173 416
Fragments arranged by size:
416 337 173 148
Bme1580I G_kGCm'C
Cuts at: 0 481 585 773 1002 1029 1074
Size: 481 104 188 229 27 45
Fragments arranged by size:
481 229 188 104 45 27
BmgBI CAC'GTC
Cuts at: 0 642 1074
Size: 642 432
BmrI ACTGGGnnnn_n'
Cuts at: 0 476 1074
Size: 476 598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 1074
Size: 150 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 852 1062 1074
Size: 70 782 210 12
Fragments arranged by size:
782 210 70 12
Bpu10I CC'TnA_GC
Cuts at: 0 563 1074
Size: 563 511
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 667 1074
Size: 202 465 407
Fragments arranged by size:
465 407 202
BsaHI Gr'CG_yC
Cuts at: 0 25 308 1074
Size: 25 283 766
Fragments arranged by size:
766 283 25
BsaWI w'CCGG_w
Cuts at: 0 536 1074
Size: 536 538
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1074
Size: 538 30 506
Fragments arranged by size:
538 506 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 898 933 936 1074
Size: 337 561 35 3 138
Fragments arranged by size:
561 337 138 35 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 403 487 1074
Size: 67 336 84 587
Fragments arranged by size:
587 336 84 67
BsiEI CG_ry'CG
Cuts at: 0 20 143 371 1074
Size: 20 123 228 703
Fragments arranged by size:
703 228 123 20
BsiHKAI G_wGCw'C
Cuts at: 0 749 1074
Size: 749 325
BsmBI CGTCTCn'nnnn_
Cuts at: 0 274 1074
Size: 274 800
Bsp1286I G_dGCh'C
Cuts at: 0 131 481 578 585 749 773 841 1002
Size: 131 350 97 7 164 24 68 161
Cuts at: 1002 1029 1074
Size: 27 45
Fragments arranged by size:
350 164 161 131 97 68 45 27
24 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 210 509 1074
Size: 89 121 299 565
Fragments arranged by size:
565 299 121 89
BsrFI r'CCGG_y
Cuts at: 0 138 170 366 1074
Size: 138 32 196 708
Fragments arranged by size:
708 196 138 32
BssHII G'CGCG_C
Cuts at: 0 333 1074
Size: 333 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1074
Size: 684 390
BstYI r'GATC_y
Cuts at: 0 859 1074
Size: 859 215
BtgI C'CryG_G
Cuts at: 0 983 1074
Size: 983 91
EaeI y'GGCC_r
Cuts at: 0 17 140 368 980 986 1074
Size: 17 123 228 612 6 88
Fragments arranged by size:
612 228 123 88 17 6
EagI C'GGCC_G
Cuts at: 0 17 140 368 1074
Size: 17 123 228 706
Fragments arranged by size:
706 228 123 17
EarI CTCTTCn'nnn_
Cuts at: 0 1066 1074
Size: 1066 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 639 852 1074
Size: 639 213 222
Fragments arranged by size:
639 222 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 554 852 1047 1062 1074
Size: 70 484 298 195 15 12
Fragments arranged by size:
484 298 195 70 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 820 1074
Size: 52 768 254
Fragments arranged by size:
768 254 52
EcoO109I rG'GnC_Cy
Cuts at: 0 477 478 998 1026 1074
Size: 477 1 520 28 48
Fragments arranged by size:
520 477 48 28 1
EcoRI G'AATT_C
Cuts at: 0 891 1074
Size: 891 183
HaeII r_GCGC'y
Cuts at: 0 28 349 931 1074
Size: 28 321 582 143
Fragments arranged by size:
582 321 143 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 631 663 723 755 1074
Size: 538 32 61 32 60 32 319
Fragments arranged by size:
538 319 61 60 32 32 32
HincII GTy'rAC
Cuts at: 0 273 1074
Size: 273 801
KasI G'GCGC_C
Cuts at: 0 24 1074
Size: 24 1050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1074
Size: 974 100
MscI TGG'CCA
Cuts at: 0 982 988 1074
Size: 982 6 86
Fragments arranged by size:
982 86 6
MslI CAynn'nnrTG
Cuts at: 0 752 1074
Size: 752 322
MspA1I CmG'CkG
Cuts at: 0 16 294 1074
Size: 16 278 780
Fragments arranged by size:
780 278 16
NaeI GCC'GGC
Cuts at: 0 140 368 1074
Size: 140 228 706
Fragments arranged by size:
706 228 140
NarI GG'CG_CC
Cuts at: 0 25 1074
Size: 25 1049
NcoI C'CATG_G
Cuts at: 0 983 1074
Size: 983 91
NgoMIV G'CCGG_C
Cuts at: 0 138 366 1074
Size: 138 228 708
Fragments arranged by size:
708 228 138
NruI TCG'CGA
Cuts at: 0 413 1074
Size: 413 661
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 1074
Size: 222 852
PspOMI G'GGCC_C
Cuts at: 0 477 581 998 1025 1074
Size: 477 104 417 27 49
Fragments arranged by size:
477 417 104 49 27
PstI C_TGCA'G
Cuts at: 0 299 1074
Size: 299 775
PvuII CAG'CTG
Cuts at: 0 294 1074
Size: 294 780
SalI G'TCGA_C
Cuts at: 0 271 1074
Size: 271 803
SfcI C'TryA_G
Cuts at: 0 295 1074
Size: 295 779
SfoI GGC'GCC
Cuts at: 0 26 1074
Size: 26 1048
StyI C'CwwG_G
Cuts at: 0 830 983 1074
Size: 830 153 91
Fragments arranged by size:
830 153 91
TatI w'GTAC_w
Cuts at: 0 110 1074
Size: 110 964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 849 1074
Size: 684 165 225
Fragments arranged by size:
684 225 165
Enzymes that do cut:
AarI AccI AcuI AhdI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BstXI
BstYI BtgI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI PflMI
PspOMI PstI PvuII SalI SfcI SfoI StyI TatI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BpuEI BsaAI BsaBI BseYI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI ZraI