Restriction map for
pFN21AE0788
(Linear) (Six-base) MAPSORT of: KIEE0788.seq Check: 4647 from: 1 to: 1257
KIEE0788.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 397 610 1250 1257
Size: 397 213 640 7
Fragments arranged by size:
640 397 213 7
AatII G_ACGT'C
Cuts at: 0 575 731 1257
Size: 575 156 526
Fragments arranged by size:
575 526 156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 532 718 1236 1257
Size: 70 462 186 518 21
Fragments arranged by size:
518 462 186 70 21
AfeI AGC'GCT
Cuts at: 0 334 1144 1257
Size: 334 810 113
Fragments arranged by size:
810 334 113
AhdI GACnn_n'nnGTC
Cuts at: 0 1059 1170 1257
Size: 1059 111 87
Fragments arranged by size:
1059 111 87
AlwNI CAG_nnn'CTG
Cuts at: 0 27 285 1257
Size: 27 258 972
Fragments arranged by size:
972 258 27
ApaI G_GGCC'C
Cuts at: 0 637 1257
Size: 637 620
AvaI C'yCGr_G
Cuts at: 0 643 856 1047 1257
Size: 643 213 191 210
Fragments arranged by size:
643 213 210 191
BanI G'GyrC_C
Cuts at: 0 385 598 852 1257
Size: 385 213 254 405
Fragments arranged by size:
405 385 254 213
BanII G_rGCy'C
Cuts at: 0 181 230 593 637 1019 1257
Size: 181 49 363 44 382 238
Fragments arranged by size:
382 363 238 181 49 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 427 567 1204 1257
Size: 427 140 637 53
Fragments arranged by size:
637 427 140 53
BglI GCCn_nnn'nGGC
Cuts at: 0 850 1257
Size: 850 407
Bme1580I G_kGCm'C
Cuts at: 0 601 637 1257
Size: 601 36 620
Fragments arranged by size:
620 601 36
BmgBI CAC'GTC
Cuts at: 0 432 1167 1257
Size: 432 735 90
Fragments arranged by size:
735 432 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1177 1257
Size: 379 798 80
Fragments arranged by size:
798 379 80
Bpu10I CC'TnA_GC
Cuts at: 0 1228 1257
Size: 1228 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1257
Size: 601 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 1095 1257
Size: 1095 162
BsaHI Gr'CG_yC
Cuts at: 0 7 572 728 1171 1223 1257
Size: 7 565 156 443 52 34
Fragments arranged by size:
565 443 156 52 34 7
BsaWI w'CCGG_w
Cuts at: 0 416 1257
Size: 416 841
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 1257
Size: 487 770
BseYI C'CCAG_C
Cuts at: 0 107 222 1257
Size: 107 115 1035
Fragments arranged by size:
1035 115 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1257
Size: 559 698
BsiEI CG_ry'CG
Cuts at: 0 798 1257
Size: 798 459
BsiHKAI G_wGCw'C
Cuts at: 0 181 593 1257
Size: 181 412 664
Fragments arranged by size:
664 412 181
BsmI GAATG_Cn'
Cuts at: 0 650 1257
Size: 650 607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1144 1257
Size: 1144 113
Bsp1286I G_dGCh'C
Cuts at: 0 181 230 593 601 637 1019 1257
Size: 181 49 363 8 36 382 238
Fragments arranged by size:
382 363 238 181 49 36 8
BspEI T'CCGG_A
Cuts at: 0 416 1257
Size: 416 841
BspHI T'CATG_A
Cuts at: 0 296 1257
Size: 296 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 610 1250 1257
Size: 397 213 640 7
Fragments arranged by size:
640 397 213 7
BsrBI CCG'CTC
Cuts at: 0 161 759 1257
Size: 161 598 498
Fragments arranged by size:
598 498 161
BsrDI GCAATG_nn'
Cuts at: 0 434 1257
Size: 434 823
BsrFI r'CCGG_y
Cuts at: 0 424 832 1257
Size: 424 408 425
Fragments arranged by size:
425 424 408
BtgI C'CryG_G
Cuts at: 0 584 1210 1257
Size: 584 626 47
Fragments arranged by size:
626 584 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 564 1257
Size: 564 693
BtsI GCAGTG_nn'
Cuts at: 0 547 1257
Size: 547 710
DrdI GACnn_nn'nnGTC
Cuts at: 0 988 1257
Size: 988 269
EaeI y'GGCC_r
Cuts at: 0 140 393 426 795 1257
Size: 140 253 33 369 462
Fragments arranged by size:
462 369 253 140 33
EagI C'GGCC_G
Cuts at: 0 795 1257
Size: 795 462
EarI CTCTTCn'nnn_
Cuts at: 0 508 1007 1093 1257
Size: 508 499 86 164
Fragments arranged by size:
508 499 164 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 1257
Size: 90 1167
EcoICRI GAG'CTC
Cuts at: 0 179 591 1257
Size: 179 412 666
Fragments arranged by size:
666 412 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 379 532 718 1177 1236 1257
Size: 70 309 153 186 459 59 21
Fragments arranged by size:
459 309 186 153 70 59 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1251 1257
Size: 1251 6
EcoO109I rG'GnC_Cy
Cuts at: 0 633 634 990 1257
Size: 633 1 356 267
Fragments arranged by size:
633 356 267 1
EcoRV GAT'ATC
Cuts at: 0 99 1257
Size: 99 1158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 999 1096 1257
Size: 429 570 97 161
Fragments arranged by size:
570 429 161 97
HaeII r_GCGC'y
Cuts at: 0 336 459 688 833 1146 1257
Size: 336 123 229 145 313 111
Fragments arranged by size:
336 313 229 145 123 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1156 1188 1257
Size: 1045 32 79 32 69
Fragments arranged by size:
1045 79 69 32 32
HincII GTy'rAC
Cuts at: 0 236 1257
Size: 236 1021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1257
Size: 764 493
MscI TGG'CCA
Cuts at: 0 142 1257
Size: 142 1115
MslI CAynn'nnrTG
Cuts at: 0 490 583 1257
Size: 490 93 674
Fragments arranged by size:
674 490 93
MspA1I CmG'CkG
Cuts at: 0 111 138 383 794 1212 1257
Size: 111 27 245 411 418 45
Fragments arranged by size:
418 411 245 111 45 27
NaeI GCC'GGC
Cuts at: 0 834 1257
Size: 834 423
NcoI C'CATG_G
Cuts at: 0 584 1257
Size: 584 673
NgoMIV G'CCGG_C
Cuts at: 0 832 1257
Size: 832 425
NotI GC'GGCC_GC
Cuts at: 0 795 1257
Size: 795 462
NspI r_CATG'y
Cuts at: 0 222 1257
Size: 222 1035
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 1257
Size: 222 1035
PfoI T'CCnGG_A
Cuts at: 0 460 1257
Size: 460 797
PpuMI rG'GwC_Cy
Cuts at: 0 990 1257
Size: 990 267
PspOMI G'GGCC_C
Cuts at: 0 633 1257
Size: 633 624
PstI C_TGCA'G
Cuts at: 0 131 290 1257
Size: 131 159 967
Fragments arranged by size:
967 159 131
SacI G_AGCT'C
Cuts at: 0 181 593 1257
Size: 181 412 664
Fragments arranged by size:
664 412 181
SacII CC_GC'GG
Cuts at: 0 1213 1257
Size: 1213 44
SapI GCTCTTCn'nnn_
Cuts at: 0 508 1007 1257
Size: 508 499 250
Fragments arranged by size:
508 499 250
SfcI C'TryA_G
Cuts at: 0 127 286 1006 1257
Size: 127 159 720 251
Fragments arranged by size:
720 251 159 127
SmlI C'TyrA_G
Cuts at: 0 616 1257
Size: 616 641
StyI C'CwwG_G
Cuts at: 0 584 1257
Size: 584 673
TatI w'GTAC_w
Cuts at: 0 689 1257
Size: 689 568
Tth111I GACn'n_nGTC
Cuts at: 0 1203 1257
Size: 1203 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 747 1257
Size: 591 156 510
Fragments arranged by size:
591 510 156
ZraI GAC'GTC
Cuts at: 0 573 729 1257
Size: 573 156 528
Fragments arranged by size:
573 528 156
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AlwNI ApaI AvaI
BanI BanII BbsI BglI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NotI NspI PflMI PfoI PpuMI PspOMI
PstI SacI SacII SapI SfcI SmlI StyI TatI
Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BsaAI BsaBI BsaXI BsiWI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI XbaI XhoI XmaI
XmnI