(Circular) (Six-base) MAPSORT of: pFN21AE0760.seq Check: 3299 from: 1 to: 6001 pFN21AE0760 6001 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2408 3344 1937 Size: 471 936 4594 Fragments arranged by size: 4594 936 471 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 2403 4900 5877 1203 Size: 1200 2497 977 1327 Fragments arranged by size: 2497 1327 1200 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 2709 2793 3213 1166 Size: 1543 84 420 3954 Fragments arranged by size: 3954 1543 420 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 3744 5316 3744 Size: 1572 4429 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2176 2203 2532 2784 2868 3036 3204 3288 Size: 683 27 329 252 84 168 168 84 Cuts at: 3288 3310 3536 3688 5797 1493 Size: 22 226 152 2109 1697 Fragments arranged by size: 2109 1697 683 329 252 226 168 168 152 84 84 27 22 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 4777 618 Size: 325 217 627 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 3098 3320 1391 Size: 892 815 222 4072 Fragments arranged by size: 4072 892 815 222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2406 928 Size: 971 507 4523 Fragments arranged by size: 4523 971 507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4192 4226 1903 Size: 34 50 34 2171 34 3678 Fragments arranged by size: 3678 2171 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2104 3893 5103 2104 Size: 1789 1210 3002 Fragments arranged by size: 3002 1789 1210 BclI T'GATC_A Cuts at: 1548 2167 2773 1548 Size: 619 606 4776 Fragments arranged by size: 4776 619 606 BfrBI ATG'CAT Cuts at: 2071 2956 2071 Size: 885 5116 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2323 5996 2323 Size: 3673 2328 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 4062 5218 1790 Size: 2272 1156 2573 Fragments arranged by size: 2573 2272 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3732 4690 442 Size: 416 53 83 399 24 2315 958 1753 Fragments arranged by size: 2315 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4661 1239 Size: 3422 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2124 2914 4126 4991 5289 2124 Size: 790 1212 865 298 2836 Fragments arranged by size: 2836 1212 865 790 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 4664 882 Size: 617 837 2328 2219 Fragments arranged by size: 2328 2219 837 617 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3808 4190 4825 275 Size: 53 83 186 936 2275 382 635 1451 Fragments arranged by size: 2275 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2007 2072 1186 Size: 821 65 5115 Fragments arranged by size: 5115 821 65 BseYI C'CCAG_C Cuts at: 5204 5700 5204 Size: 496 5505 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3098 4062 4147 5218 3098 Size: 964 85 1071 3881 Fragments arranged by size: 3881 1071 964 85 BsmI GAATG_Cn' Cuts at: 2073 2159 2958 3512 3605 2073 Size: 86 799 554 93 4469 Fragments arranged by size: 4469 799 554 93 86 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 3098 3320 4062 4147 5218 1391 Size: 399 493 815 222 742 85 1071 2174 Fragments arranged by size: 2174 1071 815 742 493 399 222 85 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3356 844 Size: 2512 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 4645 4832 1700 Size: 110 294 2541 187 2869 Fragments arranged by size: 2869 2541 294 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2323 3331 3698 4083 4100 5552 5638 5650 Size: 351 1008 367 385 17 1452 86 12 Cuts at: 5650 5996 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1008 385 367 351 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2468 513 Size: 552 1403 4046 Fragments arranged by size: 4046 1403 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2993 3517 4311 4331 2993 Size: 524 794 20 4663 Fragments arranged by size: 4663 794 524 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2376 5008 809 Size: 507 1060 2632 1802 Fragments arranged by size: 2632 1802 1060 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3448 4337 5966 Size: 54 1425 50 275 18 1618 889 1629 Cuts at: 5966 8 Size: 43 Fragments arranged by size: 1629 1618 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2360 2454 3931 1353 Size: 696 311 94 1477 3423 Fragments arranged by size: 3423 1477 696 311 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3096 3096 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4064 4661 5448 5716 1239 Size: 393 2432 597 787 268 1524 Fragments arranged by size: 2432 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2532 2784 2868 3036 3204 3288 3310 3688 2532 Size: 252 84 168 168 84 22 378 4845 Fragments arranged by size: 4845 378 252 168 168 84 84 22 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2485 2517 4642 Size: 32 135 32 743 32 138 32 2125 Cuts at: 4642 4674 4716 4748 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2125 743 138 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 2545 5115 5299 1305 Size: 873 367 2570 184 2007 Fragments arranged by size: 2570 2007 873 367 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4179 Fragments arranged by size: 4179 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2473 2541 2709 2793 2877 2961 3045 Size: 648 1307 68 168 84 84 84 84 Cuts at: 3045 3129 3145 3213 3297 3959 4318 4477 518 Size: 84 16 68 84 662 359 159 2042 Fragments arranged by size: 2042 1307 662 648 359 168 159 84 84 84 84 84 84 68 68 16 MspA1I CmG'CkG Cuts at: 1606 2249 3417 4094 5242 5487 1606 Size: 643 1168 677 1148 245 2120 Fragments arranged by size: 2120 1168 1148 677 643 245 NcoI C'CATG_G Cuts at: 513 1065 2468 513 Size: 552 1403 4046 Fragments arranged by size: 4046 1403 552 NdeI CA'TA_TG Cuts at: 387 3142 387 Size: 2755 3246 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NsiI A_TGCA'T Cuts at: 2073 2958 2073 Size: 885 5116 NspI r_CATG'y Cuts at: 1132 1229 2407 3359 4904 1132 Size: 97 1178 952 1545 2229 Fragments arranged by size: 2229 1545 1178 952 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 2403 4900 2403 Size: 2497 3504 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6001 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 3353 830 Size: 2523 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2249 2249 Size: 6001 SacI G_AGCT'C Cuts at: 3098 3098 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SexAI A'CCwGG_T Cuts at: 2214 2214 Size: 6001 SfcI C'TryA_G Cuts at: 826 1045 3349 4484 5165 5356 826 Size: 219 2304 1135 681 191 1471 Fragments arranged by size: 2304 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2139 2929 4105 5006 5268 820 Size: 197 926 196 790 1176 901 262 1553 Fragments arranged by size: 1553 1176 926 901 790 262 197 196 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 2352 3925 5 Size: 47 2300 1573 2081 Fragments arranged by size: 2300 2081 1573 47 StuI AGG'CCT Cuts at: 1517 2313 2999 3083 3167 1517 Size: 796 686 84 84 4351 Fragments arranged by size: 4351 796 686 84 84 StyI C'CwwG_G Cuts at: 513 1065 2097 2468 3440 513 Size: 552 1032 371 972 3074 Fragments arranged by size: 3074 1032 972 552 371 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 4028 4045 4198 4383 4828 325 Size: 1255 484 1964 17 153 185 445 1498 Fragments arranged by size: 1964 1498 1255 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4247 5959 96 Size: 274 80 33 51 1443 2270 1712 138 Fragments arranged by size: 2270 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 2502 2534 5961 5993 2321 Size: 32 149 32 3427 32 2329 Fragments arranged by size: 3427 2329 149 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 3105 4130 3105 Size: 1025 4976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI