(Circular) (Six-base) MAPSORT of: pFN21AE0724.seq Check: 3758 from: 1 to: 5950 pFN21AE0724 5950 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 3293 1937 Size: 382 974 4594 Fragments arranged by size: 4594 974 382 Acc65I G'GTAC_C Cuts at: 2793 3271 2793 Size: 478 5472 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4013 5397 5665 1632 Size: 2381 1384 268 1917 Fragments arranged by size: 2381 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2724 4849 5826 1203 Size: 1521 2125 977 1327 Fragments arranged by size: 2125 1521 1327 977 AgeI A'CCGG_T Cuts at: 2274 4781 2274 Size: 2507 3443 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 2375 2407 2261 Size: 32 82 32 5804 Fragments arranged by size: 5804 82 32 32 AlwNI CAG_nnn'CTG Cuts at: 3167 3693 5265 3167 Size: 526 1572 3852 Fragments arranged by size: 3852 1572 526 ApaI G_GGCC'C Cuts at: 2135 2254 2376 2135 Size: 119 122 5709 Fragments arranged by size: 5709 122 119 ApaLI G'TGCA_C Cuts at: 2471 4007 5163 2471 Size: 1536 1156 3258 Fragments arranged by size: 3258 1536 1156 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2101 2113 2247 2507 2530 2597 3275 Size: 73 158 12 134 260 23 67 678 Cuts at: 3275 1870 Size: 4545 Fragments arranged by size: 4545 678 260 158 134 73 67 23 12 AvrII C'CTAG_G Cuts at: 2188 2188 Size: 5950 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2793 2889 3020 3271 4726 Size: 325 217 627 1006 96 131 251 1455 Cuts at: 4726 618 Size: 1842 Fragments arranged by size: 1842 1455 1006 627 325 251 217 131 96 BanII G_rGCy'C Cuts at: 1391 2102 2135 2184 2254 2376 2769 3269 1391 Size: 711 33 49 70 122 393 500 4072 Fragments arranged by size: 4072 711 500 393 122 70 49 33 BbeI G_GCGC'C Cuts at: 2893 2893 Size: 5950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4979 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2140 2174 2947 2981 4141 4175 1903 Size: 34 203 34 773 34 1160 34 3678 Fragments arranged by size: 3678 1160 773 203 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 2451 1548 Size: 903 5047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2643 4561 136 Size: 107 122 71 631 605 971 1918 1525 Fragments arranged by size: 1918 1525 971 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2291 3367 2291 Size: 1076 4874 Bme1580I G_kGCm'C Cuts at: 1790 2135 2254 2376 2475 2648 4011 5167 1790 Size: 345 119 122 99 173 1363 1156 2573 Fragments arranged by size: 2573 1363 1156 345 173 122 119 99 BmgBI CAC'GTC Cuts at: 1318 2500 1318 Size: 1182 4768 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2998 3681 4639 Size: 416 53 83 399 24 1581 683 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1581 958 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2381 2402 2729 4610 1239 Size: 1049 93 21 327 1881 2579 Fragments arranged by size: 2579 1881 1049 327 93 21 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 2753 2768 4075 4940 5238 2405 Size: 348 15 1307 865 298 3117 Fragments arranged by size: 3117 1307 865 348 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2594 4613 882 Size: 617 1095 2019 2219 Fragments arranged by size: 2219 2019 1095 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 2871 3741 493 Size: 711 1014 653 870 2702 Fragments arranged by size: 2702 1014 870 711 653 BsaBI GATnn'nnATC Cuts at: 2441 5944 2441 Size: 3503 2447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2307 2890 3757 4139 Size: 53 83 186 936 774 583 867 382 Cuts at: 4139 4774 275 Size: 635 1451 Fragments arranged by size: 1451 936 867 774 635 583 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 2274 2406 4383 4781 5055 5202 5613 2094 Size: 180 132 1977 398 274 147 411 2431 Fragments arranged by size: 2431 1977 411 398 274 180 147 132 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 2375 2405 3026 Size: 30 165 30 858 30 84 30 621 Cuts at: 3026 3056 1178 Size: 30 4072 Fragments arranged by size: 4072 858 621 165 84 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2873 3232 1186 Size: 871 816 359 3904 Fragments arranged by size: 3904 871 816 359 BseYI C'CCAG_C Cuts at: 2368 2375 2716 3045 3069 5153 5649 2368 Size: 7 341 329 24 2084 496 2669 Fragments arranged by size: 2669 2084 496 341 329 24 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 1163 Size: 573 510 4867 Fragments arranged by size: 4867 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2407 2956 3138 3400 4161 5189 Size: 275 186 406 549 182 262 761 1028 Cuts at: 5189 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 549 406 275 262 186 182 BsiHKAI G_wGCw'C Cuts at: 2102 2184 2475 2631 2841 4011 4096 5167 2102 Size: 82 291 156 210 1170 85 1071 2885 Fragments arranged by size: 2885 1170 1071 291 210 156 85 82 BsmI GAATG_Cn' Cuts at: 3124 3461 3554 3124 Size: 337 93 5520 Fragments arranged by size: 5520 337 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2135 2184 2254 2376 2475 2631 Size: 399 312 33 49 70 122 99 156 Cuts at: 2631 2648 2769 2841 3269 4011 4096 5167 1391 Size: 17 121 72 428 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 399 312 156 122 121 99 85 72 70 49 33 17 BspEI T'CCGG_A Cuts at: 2094 5613 2094 Size: 3519 2431 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2883 2934 3045 3305 844 Size: 2039 51 111 260 3489 Fragments arranged by size: 3489 2039 260 111 51 BsrBI CCG'CTC Cuts at: 1394 1823 2791 3837 1394 Size: 429 968 1046 3507 Fragments arranged by size: 3507 1046 968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2274 3137 4594 4781 1700 Size: 110 256 208 863 1457 187 2869 Fragments arranged by size: 2869 1457 863 256 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 2651 1925 Size: 726 5224 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2359 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2313 2313 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2004 2086 2352 3132 513 Size: 552 939 82 266 780 3331 Fragments arranged by size: 3331 939 780 552 266 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3466 4260 4280 2965 Size: 501 794 20 4635 Fragments arranged by size: 4635 794 501 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 2865 3135 Size: 54 1425 50 275 18 747 288 270 Cuts at: 3135 3139 3183 3397 4286 5915 8 Size: 4 44 214 889 1629 43 Fragments arranged by size: 1629 1425 889 747 288 275 270 214 54 50 44 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3135 3397 1537 Size: 275 1323 262 4090 Fragments arranged by size: 4090 1323 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 2954 3880 1353 Size: 1589 12 926 3423 Fragments arranged by size: 3423 1589 926 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4537 4911 5057 387 Size: 1064 138 585 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 585 374 146 138 EcoICRI GAG'CTC Cuts at: 2100 2182 2100 Size: 82 5868 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2381 2402 2729 4013 4610 5397 Size: 393 656 93 21 327 1284 597 787 Cuts at: 5397 5665 1239 Size: 268 1524 Fragments arranged by size: 1524 1284 787 656 597 393 327 268 93 21 EcoNI CCTnn'n_nnAGG Cuts at: 2681 2681 Size: 5950 EcoO109I rG'GnC_Cy Cuts at: 2119 2132 2251 2372 2386 2119 Size: 13 119 121 14 5683 Fragments arranged by size: 5683 121 119 14 13 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FseI GG_CCGG'CC Cuts at: 3141 3141 Size: 5950 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2298 2717 2893 3167 5097 1678 Size: 620 419 176 274 1930 2531 Fragments arranged by size: 2531 1930 620 419 274 176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 4591 4623 4665 Size: 32 135 32 1454 32 1533 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 1454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KasI G'GCGC_C Cuts at: 2889 2889 Size: 5950 KpnI G_GTAC'C Cuts at: 2797 3275 2797 Size: 478 5472 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3171 5064 5248 1305 Size: 1866 1893 184 2007 Fragments arranged by size: 2007 1893 1866 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 2867 10 Size: 54 1425 343 747 288 3093 Fragments arranged by size: 3093 1425 747 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2729 2834 3908 4267 4426 518 Size: 648 1293 270 105 1074 359 159 2042 Fragments arranged by size: 2042 1293 1074 648 359 270 159 105 MspA1I CmG'CkG Cuts at: 1606 2260 2449 3366 4043 5191 5436 1606 Size: 654 189 917 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 654 245 189 NaeI GCC'GGC Cuts at: 2068 3139 2068 Size: 1071 4879 NarI GG'CG_CC Cuts at: 2890 2890 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 2004 2352 513 Size: 552 939 348 4111 Fragments arranged by size: 4111 939 552 348 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 2066 3137 2066 Size: 1071 4879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2552 2728 3308 4853 1132 Size: 97 1323 176 580 1545 2229 Fragments arranged by size: 2229 1545 1323 580 176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 2724 4849 2724 Size: 2125 3825 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 3057 3057 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2261 2293 2375 2407 4019 4051 5532 5564 2261 Size: 32 82 32 1612 32 1481 32 2647 Fragments arranged by size: 2647 1612 1481 82 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2386 2119 Size: 267 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2131 2250 2372 2131 Size: 119 122 5709 Fragments arranged by size: 5709 122 119 PstI C_TGCA'G Cuts at: 830 3172 3240 3302 830 Size: 2342 68 62 3478 Fragments arranged by size: 3478 2342 68 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2260 2449 2260 Size: 189 5761 RsrII CG'GwC_CG Cuts at: 2330 2330 Size: 5950 SacI G_AGCT'C Cuts at: 2102 2184 2102 Size: 82 5868 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SapI GCTCTTCn'nnn_ Cuts at: 2942 2942 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 3168 3236 3298 4433 5114 5305 826 Size: 219 2123 68 62 1135 681 191 1471 Fragments arranged by size: 2123 1471 1135 681 219 191 68 62 SfoI GGC'GCC Cuts at: 2891 2891 Size: 5950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 2249 2532 3277 1872 Size: 377 283 745 4545 Fragments arranged by size: 4545 745 377 283 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2768 2783 4054 4955 5217 Size: 197 926 477 348 15 1271 901 262 Cuts at: 5217 820 Size: 1553 Fragments arranged by size: 1553 1271 926 901 477 348 262 197 15 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 2552 3308 1132 Size: 1420 756 3774 Fragments arranged by size: 3774 1420 756 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2188 2352 2580 3389 513 Size: 552 939 57 127 164 228 809 3074 Fragments arranged by size: 3074 939 809 552 228 164 127 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3977 3994 4147 4332 4777 325 Size: 1255 738 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 2586 2733 Size: 274 80 33 51 1443 366 243 147 Cuts at: 2733 4196 5908 96 Size: 1463 1712 138 Fragments arranged by size: 1712 1463 1443 366 274 243 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 2247 2530 3275 1870 Size: 377 283 745 4545 Fragments arranged by size: 4545 745 377 283 XmnI GAAnn'nnTTC Cuts at: 2936 4079 2936 Size: 1143 4807 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI XcmI