Restriction map for
pFN21AE0724
(Linear) (Six-base) MAPSORT of: KIEE0724.seq Check: 904 from: 1 to: 1245
KIEE0724.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 314 1245
Size: 314 931
Acc65I G'GTAC_C
Cuts at: 0 788 1245
Size: 788 457
AflIII A'CryG_T
Cuts at: 0 719 1245
Size: 719 526
AgeI A'CCGG_T
Cuts at: 0 269 1245
Size: 269 976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 370 402 1245
Size: 256 32 82 32 843
Fragments arranged by size:
843 256 82 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1162 1245
Size: 1162 83
ApaI G_GGCC'C
Cuts at: 0 130 249 371 1245
Size: 130 119 122 874
Fragments arranged by size:
874 130 122 119
ApaLI G'TGCA_C
Cuts at: 0 466 1245
Size: 466 779
AvaI C'yCGr_G
Cuts at: 0 96 108 242 502 525 592 1245
Size: 96 12 134 260 23 67 653
Fragments arranged by size:
653 260 134 96 67 23 12
AvrII C'CTAG_G
Cuts at: 0 183 1245
Size: 183 1062
BanI G'GyrC_C
Cuts at: 0 788 884 1015 1245
Size: 788 96 131 230
Fragments arranged by size:
788 230 131 96
BanII G_rGCy'C
Cuts at: 0 97 130 179 249 371 764 1245
Size: 97 33 49 70 122 393 481
Fragments arranged by size:
481 393 122 97 70 49 33
BbeI G_GCGC'C
Cuts at: 0 888 1245
Size: 888 357
BbvCI CC'TCA_GC
Cuts at: 0 1046 1245
Size: 1046 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 135 169 942 976 1245
Size: 135 34 773 34 269
Fragments arranged by size:
773 269 135 34 34
BclI T'GATC_A
Cuts at: 0 446 1245
Size: 446 799
BglI GCCn_nnn'nGGC
Cuts at: 0 638 1245
Size: 638 607
BlpI GC'TnA_GC
Cuts at: 0 286 1245
Size: 286 959
Bme1580I G_kGCm'C
Cuts at: 0 130 249 371 470 643 1245
Size: 130 119 122 99 173 602
Fragments arranged by size:
602 173 130 122 119 99
BmgBI CAC'GTC
Cuts at: 0 495 1245
Size: 495 750
BmrI ACTGGGnnnn_n'
Cuts at: 0 993 1245
Size: 993 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 376 397 724 1245
Size: 283 93 21 327 521
Fragments arranged by size:
521 327 283 93 21
Bpu10I CC'TnA_GC
Cuts at: 0 1046 1245
Size: 1046 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 748 763 1245
Size: 400 348 15 482
Fragments arranged by size:
482 400 348 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 589 1245
Size: 589 656
BsaAI yAC'GTr
Cuts at: 0 213 866 1245
Size: 213 653 379
Fragments arranged by size:
653 379 213
BsaBI GATnn'nnATC
Cuts at: 0 436 1245
Size: 436 809
BsaHI Gr'CG_yC
Cuts at: 0 302 885 1245
Size: 302 583 360
Fragments arranged by size:
583 360 302
BsaWI w'CCGG_w
Cuts at: 0 89 269 401 1245
Size: 89 180 132 844
Fragments arranged by size:
844 180 132 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 370 400 1021 1051 1245
Size: 256 30 84 30 621 30 194
Fragments arranged by size:
621 256 194 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 868 1227 1245
Size: 52 816 359 18
Fragments arranged by size:
816 359 52 18
BseYI C'CCAG_C
Cuts at: 0 363 370 711 1040 1064 1245
Size: 363 7 341 329 24 181
Fragments arranged by size:
363 341 329 181 24 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1245
Size: 241 1004
BsiEI CG_ry'CG
Cuts at: 0 402 951 1133 1245
Size: 402 549 182 112
Fragments arranged by size:
549 402 182 112
BsiHKAI G_wGCw'C
Cuts at: 0 97 179 470 626 836 1245
Size: 97 82 291 156 210 409
Fragments arranged by size:
409 291 210 156 97 82
BsmI GAATG_Cn'
Cuts at: 0 1119 1245
Size: 1119 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 1245
Size: 25 1220
Bsp1286I G_dGCh'C
Cuts at: 0 97 130 179 249 371 470 626 643
Size: 97 33 49 70 122 99 156 17
Cuts at: 643 764 836 1245
Size: 121 72 409
Fragments arranged by size:
409 156 122 121 99 97 72 70
49 33 17
BspEI T'CCGG_A
Cuts at: 0 89 1245
Size: 89 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 878 929 1040 1245
Size: 878 51 111 205
Fragments arranged by size:
878 205 111 51
BsrBI CCG'CTC
Cuts at: 0 786 1245
Size: 786 459
BsrFI r'CCGG_y
Cuts at: 0 61 269 1132 1245
Size: 61 208 863 113
Fragments arranged by size:
863 208 113 61
BssHII G'CGCG_C
Cuts at: 0 646 1245
Size: 646 599
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 1245
Size: 354 891
Bsu36I CC'TnA_GG
Cuts at: 0 308 1245
Size: 308 937
BtgI C'CryG_G
Cuts at: 0 81 347 1127 1245
Size: 81 266 780 118
Fragments arranged by size:
780 266 118 81
BtsI GCAGTG_nn'
Cuts at: 0 960 1245
Size: 960 285
EaeI y'GGCC_r
Cuts at: 0 572 860 1130 1134 1178 1245
Size: 572 288 270 4 44 67
Fragments arranged by size:
572 288 270 67 44 4
EagI C'GGCC_G
Cuts at: 0 1130 1245
Size: 1130 115
EarI CTCTTCn'nnn_
Cuts at: 0 937 949 1245
Size: 937 12 296
Fragments arranged by size:
937 296 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 1245
Size: 169 1076
EcoICRI GAG'CTC
Cuts at: 0 95 177 1245
Size: 95 82 1068
Fragments arranged by size:
1068 95 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 376 397 724 1245
Size: 283 93 21 327 521
Fragments arranged by size:
521 327 283 93 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 676 1245
Size: 676 569
EcoO109I rG'GnC_Cy
Cuts at: 0 114 127 246 367 381 1245
Size: 114 13 119 121 14 864
Fragments arranged by size:
864 121 119 114 14 13
FseI GG_CCGG'CC
Cuts at: 0 1136 1245
Size: 1136 109
HaeII r_GCGC'y
Cuts at: 0 293 712 888 1162 1245
Size: 293 419 176 274 83
Fragments arranged by size:
419 293 274 176 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1245
Size: 1021 32 192
Fragments arranged by size:
1021 192 32
KasI G'GCGC_C
Cuts at: 0 884 1245
Size: 884 361
KpnI G_GTAC'C
Cuts at: 0 792 1245
Size: 792 453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1245
Size: 1166 79
MscI TGG'CCA
Cuts at: 0 574 862 1245
Size: 574 288 383
Fragments arranged by size:
574 383 288
MslI CAynn'nnrTG
Cuts at: 0 454 724 829 1245
Size: 454 270 105 416
Fragments arranged by size:
454 416 270 105
MspA1I CmG'CkG
Cuts at: 0 255 444 1245
Size: 255 189 801
Fragments arranged by size:
801 255 189
NaeI GCC'GGC
Cuts at: 0 63 1134 1245
Size: 63 1071 111
Fragments arranged by size:
1071 111 63
NarI GG'CG_CC
Cuts at: 0 885 1245
Size: 885 360
NcoI C'CATG_G
Cuts at: 0 347 1245
Size: 347 898
NgoMIV G'CCGG_C
Cuts at: 0 61 1132 1245
Size: 61 1071 113
Fragments arranged by size:
1071 113 61
NspI r_CATG'y
Cuts at: 0 547 723 1245
Size: 547 176 522
Fragments arranged by size:
547 522 176
PciI A'CATG_T
Cuts at: 0 719 1245
Size: 719 526
PfoI T'CCnGG_A
Cuts at: 0 1052 1245
Size: 1052 193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 370 402 1245
Size: 256 32 82 32 843
Fragments arranged by size:
843 256 82 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 114 381 1245
Size: 114 267 864
Fragments arranged by size:
864 267 114
PspOMI G'GGCC_C
Cuts at: 0 126 245 367 1245
Size: 126 119 122 878
Fragments arranged by size:
878 126 122 119
PstI C_TGCA'G
Cuts at: 0 1167 1235 1245
Size: 1167 68 10
Fragments arranged by size:
1167 68 10
PvuII CAG'CTG
Cuts at: 0 255 444 1245
Size: 255 189 801
Fragments arranged by size:
801 255 189
RsrII CG'GwC_CG
Cuts at: 0 325 1245
Size: 325 920
SacI G_AGCT'C
Cuts at: 0 97 179 1245
Size: 97 82 1066
Fragments arranged by size:
1066 97 82
SapI GCTCTTCn'nnn_
Cuts at: 0 937 1245
Size: 937 308
SfcI C'TryA_G
Cuts at: 0 1163 1231 1245
Size: 1163 68 14
Fragments arranged by size:
1163 68 14
SfoI GGC'GCC
Cuts at: 0 886 1245
Size: 886 359
SmaI CCC'GGG
Cuts at: 0 244 527 1245
Size: 244 283 718
Fragments arranged by size:
718 283 244
SmlI C'TyrA_G
Cuts at: 0 415 763 778 1245
Size: 415 348 15 467
Fragments arranged by size:
467 415 348 15
SphI G_CATG'C
Cuts at: 0 547 1245
Size: 547 698
StyI C'CwwG_G
Cuts at: 0 56 183 347 575 1245
Size: 56 127 164 228 670
Fragments arranged by size:
670 228 164 127 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 1245
Size: 313 932
TatI w'GTAC_w
Cuts at: 0 338 581 728 1245
Size: 338 243 147 517
Fragments arranged by size:
517 338 243 147
XmaI C'CCGG_G
Cuts at: 0 242 525 1245
Size: 242 283 720
Fragments arranged by size:
720 283 242
XmnI GAAnn'nnTTC
Cuts at: 0 931 1245
Size: 931 314
Enzymes that do cut:
AccI Acc65I AflIII AgeI AloI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbvCI BcgI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BstXI Bsu36I BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PpiI
PpuMI PspOMI PstI PvuII RsrII SacI SapI SfcI
SfoI SmaI SmlI SphI StyI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AhdI AleI
AlfI ApoI AscI AseI AsiSI BaeI BamHI BbsI
BciVI BfrBI BglII BmtI BplI BsiWI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI