(Linear) (Six-base) MAPSORT of: KIEE4142.seq Check: 454 from: 1 to: 1704 KIEE4142.orf With 182 enzymes: SgfI * March 22, 2012 17:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 755 1569 1684 1704 Size: 755 814 115 20 Fragments arranged by size: 814 755 115 20 AclI AA'CG_TT Cuts at: 0 941 1704 Size: 941 763 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 831 1116 1503 1704 Size: 85 746 285 387 201 Fragments arranged by size: 746 387 285 201 85 AfeI AGC'GCT Cuts at: 0 301 1704 Size: 301 1403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 512 1704 Size: 512 1192 AlwNI CAG_nnn'CTG Cuts at: 0 747 1704 Size: 747 957 ApaI G_GGCC'C Cuts at: 0 171 1057 1642 1704 Size: 171 886 585 62 Fragments arranged by size: 886 585 171 62 AvaI C'yCGr_G Cuts at: 0 902 1284 1603 1704 Size: 902 382 319 101 Fragments arranged by size: 902 382 319 101 BanI G'GyrC_C Cuts at: 0 581 1597 1704 Size: 581 1016 107 Fragments arranged by size: 1016 581 107 BanII G_rGCy'C Cuts at: 0 171 383 679 909 1057 1642 1704 Size: 171 212 296 230 148 585 62 Fragments arranged by size: 585 296 230 212 171 148 62 BbsI GAAGACnn'nnnn_ Cuts at: 0 875 1122 1704 Size: 875 247 582 Fragments arranged by size: 875 582 247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 985 1019 1704 Size: 985 34 685 Fragments arranged by size: 985 685 34 BfrBI ATG'CAT Cuts at: 0 448 1704 Size: 448 1256 BglI GCCn_nnn'nGGC Cuts at: 0 175 763 970 1606 1646 1704 Size: 175 588 207 636 40 58 Fragments arranged by size: 636 588 207 175 58 40 BlpI GC'TnA_GC Cuts at: 0 521 1704 Size: 521 1183 Bme1580I G_kGCm'C Cuts at: 0 171 830 1057 1602 1642 1704 Size: 171 659 227 545 40 62 Fragments arranged by size: 659 545 227 171 62 40 BmgBI CAC'GTC Cuts at: 0 1128 1367 1704 Size: 1128 239 337 Fragments arranged by size: 1128 337 239 BmrI ACTGGGnnnn_n' Cuts at: 0 1213 1704 Size: 1213 491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 244 277 1164 1276 1615 1704 Size: 115 129 33 887 112 339 89 Fragments arranged by size: 887 339 129 115 112 89 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1704 Size: 673 1031 BsaI GGTCTCn'nnnn_ Cuts at: 0 556 1704 Size: 556 1148 BsaBI GATnn'nnATC Cuts at: 0 79 1704 Size: 79 1625 BsaHI Gr'CG_yC Cuts at: 0 311 1704 Size: 311 1393 BsaWI w'CCGG_w Cuts at: 0 188 394 600 1038 1565 1704 Size: 188 206 206 438 527 139 Fragments arranged by size: 527 438 206 206 188 139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1195 1225 1578 1608 1704 Size: 1195 30 353 30 96 Fragments arranged by size: 1195 353 96 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1173 1176 1207 1210 1266 1442 1462 1599 Size: 1173 3 31 3 56 176 20 137 Cuts at: 1599 1704 Size: 105 Fragments arranged by size: 1173 176 137 105 56 31 20 3 3 BseYI C'CCAG_C Cuts at: 0 1316 1704 Size: 1316 388 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 226 732 1003 1704 Size: 69 157 506 271 701 Fragments arranged by size: 701 506 271 157 69 BsiEI CG_ry'CG Cuts at: 0 1472 1704 Size: 1472 232 BsiHKAI G_wGCw'C Cuts at: 0 263 527 845 1704 Size: 263 264 318 859 Fragments arranged by size: 859 318 264 263 BsmI GAATG_Cn' Cuts at: 0 780 1001 1704 Size: 780 221 703 Fragments arranged by size: 780 703 221 Bsp1286I G_dGCh'C Cuts at: 0 171 263 383 527 679 830 845 909 Size: 171 92 120 144 152 151 15 64 Cuts at: 909 1057 1602 1642 1704 Size: 148 545 40 62 Fragments arranged by size: 545 171 152 151 148 144 120 92 64 62 40 15 BspEI T'CCGG_A Cuts at: 0 394 600 1565 1704 Size: 394 206 965 139 Fragments arranged by size: 965 394 206 139 BspHI T'CATG_A Cuts at: 0 1016 1704 Size: 1016 688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 534 755 1569 1684 1704 Size: 534 221 814 115 20 Fragments arranged by size: 814 534 221 115 20 BsrBI CCG'CTC Cuts at: 0 1506 1704 Size: 1506 198 BsrFI r'CCGG_y Cuts at: 0 12 565 641 1704 Size: 12 553 76 1063 Fragments arranged by size: 1063 553 76 12 BstYI r'GATC_y Cuts at: 0 1431 1704 Size: 1431 273 BtgI C'CryG_G Cuts at: 0 482 671 989 1472 1704 Size: 482 189 318 483 232 Fragments arranged by size: 483 482 318 232 189 EaeI y'GGCC_r Cuts at: 0 698 1156 1469 1704 Size: 698 458 313 235 Fragments arranged by size: 698 458 313 235 EagI C'GGCC_G Cuts at: 0 1469 1704 Size: 1469 235 EarI CTCTTCn'nnn_ Cuts at: 0 19 40 1704 Size: 19 21 1664 Fragments arranged by size: 1664 21 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 525 1439 1704 Size: 525 914 265 Fragments arranged by size: 914 525 265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 115 244 277 831 1116 1164 1276 Size: 85 30 129 33 554 285 48 112 Cuts at: 1276 1503 1615 1704 Size: 227 112 89 Fragments arranged by size: 554 285 227 129 112 112 89 85 48 33 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 97 1633 1704 Size: 97 1536 71 Fragments arranged by size: 1536 97 71 EcoO109I rG'GnC_Cy Cuts at: 0 167 325 1053 1054 1638 1639 1704 Size: 167 158 728 1 584 1 65 Fragments arranged by size: 728 584 167 158 65 1 1 FspI TGC'GCA Cuts at: 0 1526 1704 Size: 1526 178 HaeII r_GCGC'y Cuts at: 0 223 303 414 1305 1552 1704 Size: 223 80 111 891 247 152 Fragments arranged by size: 891 247 223 152 111 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 916 948 1704 Size: 916 32 756 Fragments arranged by size: 916 756 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 236 1181 1704 Size: 236 945 523 Fragments arranged by size: 945 523 236 MscI TGG'CCA Cuts at: 0 700 1704 Size: 700 1004 MspA1I CmG'CkG Cuts at: 0 183 231 484 716 1316 1542 1704 Size: 183 48 253 232 600 226 162 Fragments arranged by size: 600 253 232 226 183 162 48 NaeI GCC'GGC Cuts at: 0 14 1704 Size: 14 1690 NcoI C'CATG_G Cuts at: 0 989 1704 Size: 989 715 NgoMIV G'CCGG_C Cuts at: 0 12 1704 Size: 12 1692 NsiI A_TGCA'T Cuts at: 0 450 1704 Size: 450 1254 NspI r_CATG'y Cuts at: 0 147 1704 Size: 147 1557 PasI CC'CwG_GG Cuts at: 0 722 768 1704 Size: 722 46 936 Fragments arranged by size: 936 722 46 PfoI T'CCnGG_A Cuts at: 0 1253 1631 1704 Size: 1253 378 73 Fragments arranged by size: 1253 378 73 PspOMI G'GGCC_C Cuts at: 0 167 1053 1638 1704 Size: 167 886 585 66 Fragments arranged by size: 886 585 167 66 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 271 303 1704 Size: 271 32 1401 Fragments arranged by size: 1401 271 32 PstI C_TGCA'G Cuts at: 0 1052 1704 Size: 1052 652 PvuII CAG'CTG Cuts at: 0 183 1316 1542 1704 Size: 183 1133 226 162 Fragments arranged by size: 1133 226 183 162 SacII CC_GC'GG Cuts at: 0 485 1704 Size: 485 1219 SapI GCTCTTCn'nnn_ Cuts at: 0 19 1704 Size: 19 1685 SbfI CC_TGCA'GG Cuts at: 0 1052 1704 Size: 1052 652 SfcI C'TryA_G Cuts at: 0 1048 1704 Size: 1048 656 SmaI CCC'GGG Cuts at: 0 904 1286 1704 Size: 904 382 418 Fragments arranged by size: 904 418 382 SmlI C'TyrA_G Cuts at: 0 688 1704 Size: 688 1016 SrfI GCCC'GGGC Cuts at: 0 1286 1704 Size: 1286 418 StyI C'CwwG_G Cuts at: 0 347 695 989 1704 Size: 347 348 294 715 Fragments arranged by size: 715 348 347 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 771 1704 Size: 771 933 TatI w'GTAC_w Cuts at: 0 131 1704 Size: 131 1573 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 517 549 1195 1222 1227 1254 1578 1610 Size: 517 32 646 27 5 27 324 32 Cuts at: 1610 1704 Size: 94 Fragments arranged by size: 646 517 324 94 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 0 271 1486 1704 Size: 271 1215 218 Fragments arranged by size: 1215 271 218 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 219 1092 1639 1704 Size: 219 873 547 65 Fragments arranged by size: 873 547 219 65 XmaI C'CCGG_G Cuts at: 0 902 1284 1704 Size: 902 382 420 Fragments arranged by size: 902 420 382 Enzymes that do cut: AarI AclI AcuI AfeI AlfI AlwNI ApaI AvaI BanI BanII BbsI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstYI BtgI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PfoI PspOMI PsrI PstI PvuII SacII SapI SbfI SfcI SmaI SmlI SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BglII BmtI BplI Bpu10I BsaAI BsiWI BsmBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NarI NdeI NheI NotI NruI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI XmnI ZraI