(Circular) (Six-base) MAPSORT of: pFN21AE0717.seq Check: 2303 from: 1 to: 5620 pFN21AE0717 5620 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 3683 5067 5335 1632 Size: 629 1422 1384 268 1917 Fragments arranged by size: 1917 1422 1384 629 268 AfeI AGC'GCT Cuts at: 2141 2141 Size: 5620 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 2404 4349 2404 Size: 1945 3675 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2615 2647 2615 Size: 32 5588 AlwNI CAG_nnn'CTG Cuts at: 2844 3363 4935 2844 Size: 519 1572 3529 Fragments arranged by size: 3529 1572 519 ApaI G_GGCC'C Cuts at: 2805 2805 Size: 5620 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2011 2929 3155 3307 5416 1493 Size: 518 918 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 518 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2256 2577 2945 1870 Size: 73 313 321 368 4545 Fragments arranged by size: 4545 368 321 313 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 2369 2941 4396 618 Size: 325 217 627 492 90 572 1455 1842 Fragments arranged by size: 1842 1455 627 572 492 325 217 90 BanII G_rGCy'C Cuts at: 1391 2805 2939 1391 Size: 1414 134 4072 Fragments arranged by size: 4072 1414 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BbvCI CC'TCA_GC Cuts at: 2334 2334 Size: 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2375 2409 2899 2933 3811 3845 1903 Size: 34 438 34 490 34 878 34 3678 Fragments arranged by size: 3678 878 490 438 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2076 2198 2592 Size: 107 122 71 631 605 404 122 394 Cuts at: 2592 4231 136 Size: 1639 1525 Fragments arranged by size: 1639 1525 631 605 404 394 122 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 2284 2805 3681 4837 1790 Size: 494 521 876 1156 2573 Fragments arranged by size: 2573 1156 876 521 494 BmgBI CAC'GTC Cuts at: 1318 2329 1318 Size: 1011 4609 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4280 1239 Size: 3041 2579 Bpu10I CC'TnA_GC Cuts at: 2334 2334 Size: 5620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3745 4610 4908 3745 Size: 865 298 4457 Fragments arranged by size: 4457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2610 4283 882 Size: 617 1111 1673 2219 Fragments arranged by size: 2219 1673 1111 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 3427 3809 4444 Size: 53 83 186 936 657 1237 382 635 Cuts at: 4444 275 Size: 1451 Fragments arranged by size: 1451 1237 936 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2546 4053 4451 4725 4872 5283 2546 Size: 1507 398 274 147 411 2883 Fragments arranged by size: 2883 1507 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 1186 Size: 1009 4611 BseYI C'CCAG_C Cuts at: 2844 4823 5319 2844 Size: 1979 496 3145 Fragments arranged by size: 3145 1979 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 1163 Size: 573 336 4711 Fragments arranged by size: 4711 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 2596 2854 3070 3831 4859 Size: 275 186 586 9 258 216 761 1028 Cuts at: 4859 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 586 275 258 216 186 9 BsiHKAI G_wGCw'C Cuts at: 2355 3681 3766 4837 2355 Size: 1326 85 1071 3138 Fragments arranged by size: 3138 1326 1071 85 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 BsmBI CGTCTCn'nnnn_ Cuts at: 2319 2319 Size: 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2355 2805 2939 3681 3766 4837 Size: 399 494 71 450 134 742 85 1071 Cuts at: 4837 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 494 450 399 134 85 71 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2814 3507 1394 Size: 429 991 693 3507 Fragments arranged by size: 3507 991 693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2950 3317 3702 3719 5171 5257 5269 5615 Size: 978 367 385 17 1452 86 12 346 Cuts at: 5615 1972 Size: 1977 Fragments arranged by size: 1977 1452 978 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2196 2217 2373 2469 513 Size: 552 939 192 21 156 96 3664 Fragments arranged by size: 3664 939 552 192 156 96 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2486 487 Size: 683 56 1260 3621 Fragments arranged by size: 3621 1260 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2401 2436 529 Size: 35 1837 35 3713 Fragments arranged by size: 3713 1837 35 35 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2062 4627 809 Size: 507 746 2565 1802 Fragments arranged by size: 2565 1802 746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2199 2584 Size: 54 1425 50 275 18 247 122 385 Cuts at: 2584 2593 3067 3956 5585 8 Size: 9 474 889 1629 43 Fragments arranged by size: 1629 1425 889 474 385 275 247 122 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2584 2593 3067 1537 Size: 275 772 9 474 4090 Fragments arranged by size: 4090 772 474 275 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 3550 1353 Size: 1451 746 3423 Fragments arranged by size: 3423 1451 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2201 2697 4207 4581 4727 387 Size: 1064 138 612 496 1510 374 146 1280 Fragments arranged by size: 1510 1280 1064 612 496 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 3683 4280 5067 5335 1239 Size: 393 629 1422 597 787 268 1524 Fragments arranged by size: 1524 1422 787 629 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2874 2633 Size: 241 5379 EcoRI G'AATT_C Cuts at: 2011 2929 3307 2011 Size: 918 378 4324 Fragments arranged by size: 4324 918 378 EcoRV GAT'ATC Cuts at: 2539 2539 Size: 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 5620 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 2143 4767 1678 Size: 465 2624 2531 Fragments arranged by size: 2624 2531 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 2528 2560 4261 Size: 32 135 32 818 32 106 32 1701 Cuts at: 4261 4293 4335 4367 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1701 818 135 106 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2290 4734 4918 1305 Size: 985 2444 184 2007 Fragments arranged by size: 2444 2007 985 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3798 Fragments arranged by size: 3798 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3578 3937 4096 518 Size: 648 2412 359 159 2042 Fragments arranged by size: 2412 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2471 3036 3713 4861 5106 1606 Size: 865 565 677 1148 245 2120 Fragments arranged by size: 2120 1148 865 677 565 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2196 513 Size: 552 939 192 3937 Fragments arranged by size: 3937 939 552 192 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2218 2506 1108 Size: 1110 288 4222 Fragments arranged by size: 4222 1110 288 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PspOMI G'GGCC_C Cuts at: 2801 2801 Size: 5620 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SacII CC_GC'GG Cuts at: 2472 2472 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2776 2968 4103 4784 4975 826 Size: 219 1731 192 1135 681 191 1471 Fragments arranged by size: 1731 1471 1135 681 219 192 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2076 2592 2076 Size: 516 5104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2258 2579 2947 1872 Size: 386 321 368 4545 Fragments arranged by size: 4545 386 368 321 SmlI C'TyrA_G Cuts at: 820 1017 1943 3724 4625 4887 820 Size: 197 926 1781 901 262 1553 Fragments arranged by size: 1781 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2090 2316 1517 Size: 573 226 4821 Fragments arranged by size: 4821 573 226 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 2289 2529 2634 3059 513 Size: 552 939 192 93 240 105 425 3074 Fragments arranged by size: 3074 939 552 425 240 192 105 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3647 3664 3817 4002 4447 325 Size: 1255 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2256 2577 2945 1870 Size: 386 321 368 4545 Fragments arranged by size: 4545 386 368 321 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI