(Circular) (Six-base) MAPSORT of: pFN21AE0707.seq Check: 8184 from: 1 to: 5983 pFN21AE0707 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2858 2858 Size: 5983 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2287 4046 5430 5698 1632 Size: 655 1759 1384 268 1917 Fragments arranged by size: 1917 1759 1384 655 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AlwNI CAG_nnn'CTG Cuts at: 3726 5298 3726 Size: 1572 4411 ApaI G_GGCC'C Cuts at: 2166 2533 2639 2683 2938 2166 Size: 367 106 44 255 5211 Fragments arranged by size: 5211 367 255 106 44 ApaLI G'TGCA_C Cuts at: 2495 3014 4040 5196 2495 Size: 519 1026 1156 3282 Fragments arranged by size: 3282 1156 1026 519 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3122 3308 1870 Size: 73 1179 186 4545 Fragments arranged by size: 4545 1179 186 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2138 2171 2138 Size: 33 5950 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3304 4759 618 Size: 325 217 627 1517 1455 1842 Fragments arranged by size: 1842 1517 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2166 2358 2533 2639 2683 2825 2938 3050 Size: 775 192 175 106 44 142 113 112 Cuts at: 3050 3212 3302 1391 Size: 162 90 4072 Fragments arranged by size: 4072 775 192 175 162 142 113 112 106 90 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5012 BbvCI CC'TCA_GC Cuts at: 3170 3170 Size: 5983 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2852 2886 4174 4208 1903 Size: 34 915 34 1288 34 3678 Fragments arranged by size: 3678 1288 915 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2604 4594 136 Size: 107 122 71 631 605 932 1990 1525 Fragments arranged by size: 1990 1525 932 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2106 2166 2499 2533 2628 2639 2683 2938 Size: 316 60 333 34 95 11 44 255 Cuts at: 2938 3018 4044 5200 1790 Size: 80 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 333 316 255 95 80 60 44 34 11 BmgBI CAC'GTC Cuts at: 1318 3019 1318 Size: 1701 4282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2633 3714 4672 Size: 416 53 83 399 24 1216 1081 958 Cuts at: 4672 442 Size: 1753 Fragments arranged by size: 1753 1216 1081 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2372 2489 2843 4643 1239 Size: 822 311 117 354 1800 2579 Fragments arranged by size: 2579 1800 822 354 311 117 Bpu10I CC'TnA_GC Cuts at: 2468 3170 2468 Size: 702 5281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 4108 4973 5271 2135 Size: 1973 865 298 2847 Fragments arranged by size: 2847 1973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2037 3092 3263 4646 882 Size: 617 538 1055 171 1383 2219 Fragments arranged by size: 2219 1383 1055 617 538 171 BsaAI yAC'GTr Cuts at: 493 1204 2623 3013 3774 493 Size: 711 1419 390 761 2702 Fragments arranged by size: 2702 1419 761 711 390 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3008 3038 3101 3131 1178 Size: 30 165 30 1605 30 63 30 4030 Fragments arranged by size: 4030 1605 165 63 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 1186 Size: 815 5168 BseYI C'CCAG_C Cuts at: 2166 2389 2931 3222 3239 5186 5682 2166 Size: 223 542 291 17 1947 496 2467 Fragments arranged by size: 2467 1947 542 496 291 223 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2585 2603 2720 2978 1163 Size: 573 849 18 117 258 4168 Fragments arranged by size: 4168 849 573 258 117 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2872 2953 3433 4194 5222 1540 Size: 275 186 871 81 480 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 480 275 186 81 BsiHKAI G_wGCw'C Cuts at: 2358 2454 2499 2754 3018 3069 4044 4129 5200 Size: 96 45 255 264 51 975 85 1071 Cuts at: 5200 2358 Size: 3141 Fragments arranged by size: 3141 1071 975 264 255 96 85 51 45 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2166 2358 2454 2499 2533 2628 Size: 399 316 60 192 96 45 34 95 Cuts at: 2628 2639 2683 2754 2825 2938 3018 3050 3069 Size: 11 44 71 71 113 80 32 19 Cuts at: 3069 3212 3302 4044 4129 5200 1391 Size: 143 90 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 192 143 113 96 95 90 85 80 71 71 60 45 44 34 32 19 11 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 2742 3872 5602 2742 Size: 1130 1730 3123 Fragments arranged by size: 3123 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2331 2766 2858 3338 844 Size: 1370 117 435 92 480 3489 Fragments arranged by size: 3489 1370 480 435 117 92 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2089 2242 4478 4652 65 Size: 1183 841 153 2236 174 1396 Fragments arranged by size: 2236 1396 1183 841 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2518 2518 Size: 5983 BstYI r'GATC_y Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978 Size: 1341 367 385 17 1452 86 12 346 Cuts at: 5978 1972 Size: 1977 Fragments arranged by size: 1977 1452 1341 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2115 2115 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 2073 2490 2517 2675 2733 513 Size: 552 1008 417 27 158 58 3763 Fragments arranged by size: 3763 1008 552 417 158 58 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3122 487 Size: 683 56 1896 3348 Fragments arranged by size: 3348 1896 683 56 BtsI GCAGTG_nn' Cuts at: 2095 2460 3499 4293 4313 2095 Size: 365 1039 794 20 3765 Fragments arranged by size: 3765 1039 794 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DraIII CAC_nnn'GTG Cuts at: 3198 3198 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2310 2487 Size: 54 1425 50 275 18 264 216 177 Cuts at: 2487 2736 3054 3430 4319 5948 8 Size: 249 318 376 889 1629 43 Fragments arranged by size: 1629 1425 889 376 318 275 264 249 216 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 2723 3913 1353 Size: 953 417 1190 3423 Fragments arranged by size: 3423 1190 953 417 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2660 4570 4944 5090 387 Size: 1064 138 1071 1910 374 146 1280 Fragments arranged by size: 1910 1280 1071 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2356 2356 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2287 2372 2489 2843 4046 4643 Size: 393 429 226 85 117 354 1203 597 Cuts at: 4643 5430 5698 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1203 787 597 429 393 354 268 226 117 85 EcoO109I rG'GnC_Cy Cuts at: 2162 2163 2635 2680 2935 3112 3126 2162 Size: 1 472 45 255 177 14 5019 Fragments arranged by size: 5019 472 255 177 45 14 1 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FspI TGC'GCA Cuts at: 2279 4489 2279 Size: 2210 3773 FspAI rTGC'GCAy Cuts at: 2279 2279 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2229 5130 1678 Size: 551 2901 2531 Fragments arranged by size: 2901 2531 551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3194 3226 4624 4656 4698 Size: 32 135 32 1622 32 1398 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 1622 1398 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 2023 3582 2023 Size: 1559 4424 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 5097 5281 1305 Size: 993 2799 184 2007 Fragments arranged by size: 2799 2007 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 10 Size: 54 1425 343 906 3255 Fragments arranged by size: 3255 1425 906 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 2747 3941 4300 4459 518 Size: 648 1530 51 1194 359 159 2042 Fragments arranged by size: 2042 1530 1194 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2617 2654 3222 3399 4076 5224 5469 1606 Size: 1011 37 568 177 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 677 568 245 177 37 NcoI C'CATG_G Cuts at: 513 1065 2073 2517 2733 513 Size: 552 1008 444 216 3763 Fragments arranged by size: 3763 1008 552 444 216 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2665 3341 4886 1132 Size: 97 1436 676 1545 2229 Fragments arranged by size: 2229 1545 1436 676 97 PasI CC'CwG_GG Cuts at: 1348 2054 3214 1348 Size: 706 1160 4117 Fragments arranged by size: 4117 1160 706 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 3235 1108 Size: 2127 3856 PfoI T'CCnGG_A Cuts at: 3105 3105 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2623 3013 2623 Size: 390 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2162 2529 2635 2679 2934 2162 Size: 367 106 44 255 5211 Fragments arranged by size: 5211 367 255 106 44 PstI C_TGCA'G Cuts at: 830 2830 2855 3335 830 Size: 2000 25 480 3478 Fragments arranged by size: 3478 2000 480 25 PvuI CG_AT'CG Cuts at: 2001 2872 2001 Size: 871 5112 PvuII CAG'CTG Cuts at: 2617 2654 3222 2617 Size: 37 568 5378 Fragments arranged by size: 5378 568 37 SacI G_AGCT'C Cuts at: 2358 2358 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2723 2723 Size: 5983 SbfI CC_TGCA'GG Cuts at: 2855 3335 2855 Size: 480 5503 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2826 2851 3070 3331 4466 5147 5338 Size: 219 1781 25 219 261 1135 681 191 Cuts at: 5338 826 Size: 1471 Fragments arranged by size: 1781 1471 1135 681 261 219 219 191 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 4087 4988 5250 820 Size: 197 926 207 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 207 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 2665 3341 1132 Size: 1533 676 3774 Fragments arranged by size: 3774 1533 676 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 2073 2517 2733 2811 3422 513 Size: 552 1008 444 216 78 611 3074 Fragments arranged by size: 3074 1008 611 552 444 216 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 3287 4010 4027 4180 4365 4810 Size: 1255 934 773 723 17 153 185 445 Cuts at: 4810 325 Size: 1498 Fragments arranged by size: 1498 1255 934 773 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4229 5941 96 Size: 274 80 33 51 1443 2252 1712 138 Fragments arranged by size: 2252 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3008 3040 3101 3133 5943 5975 3008 Size: 32 61 32 2810 32 3016 Fragments arranged by size: 3016 2810 61 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2954 1316 Size: 1638 4345 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3236 3236 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI