################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfaI BfrI BlnI BsaBI BsaHI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MaeI MamI MfeI MluI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1215 GGTACC G^GTACC AccIII 97 TCCGGA T^CCGGA AhdI 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 701 GGGCCC GGGCC^C Asp718I 1215 GGTACC G^GTACC AspEI 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 986 GAAGAC GAAGAC(2/6) BbvCI 207 CCTCAGC CCTCAGC(-5/-2) BcgI 1477 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 558 AGATCT A^GATCT BpuAI 986 GAAGAC GAAGAC(2/6) BsaAI 1114 TACGTG [CT]AC^GT[GA] BsaWI 97 TCCGGA [AT]^CCGG[AT] BseAI 97 TCCGGA T^CCGGA BspEI 97 TCCGGA T^CCGGA BstXI 857 CCAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 558 AGATCT [GA]^GATC[CT] DrdI 447 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 239 CCATGG C^C[GA][CT]GG Eam1105I 74 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 165 CTCTTC CTCTTC(1/4) HgaI 779 GACGC GACGC(5/10) HindIII 1299 AAGCTT A^AGCTT KpnI 1215 GGTACC GGTAC^C Ksp632I 165 CTCTTC CTCTTC(1/4) MflI 558 AGATCT [GA]^GATC[CT] MroI 97 TCCGGA T^CCGGA MslI 991 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 239 CCATGG C^CATGG NspI 497 GCATGT [GA]CATG^[CT] SapI 164 GCTCTTC GCTCTTC(1/4) SmaI 704 CCCGGG CCC^GGG SmlI 1180 CTTGAG C^T[CT][GA]AG TaqI 933 TCGA T^CGA TfiI 22 GAATC G^A[AT]TC TthHB8I 933 TCGA T^CGA XhoII 558 AGATCT [GA]^GATC[CT] XmaCI 704 CCCGGG C^CCGGG XmaI 704 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 64 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1052 CAGCACCTG AvaII 66 GGACC G^G[AT]CC 1290 GGACC BanI 1215 GGTACC G^G[CT][GA]CC 1344 GGCACC BglI 349 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 406 GCCTTTGTGGC BlpI 358 GCTGAGC GC^T[ACGT]AGC 1296 GCTAAGC BpmI 270 CTGGAG CTGGAG(16/14) 1044 CTGGAG Bpu1102I 358 GCTGAGC GC^T[ACGT]AGC 1296 GCTAAGC BsaI 445 GAGACC GGTCTC(1/5) 1018 GAGACC BsmBI 81 CGTCTC CGTCTC(1/5) 606 GAGACG BsrBI 9 GAGCGG CCGCTC(-3/-3) 1119 GAGCGG CelII 358 GCTGAGC GC^T[ACGT]AGC 1296 GCTAAGC DpnII 54 GATC ^GATC 559 GATC MaeII 1115 ACGT A^CGT 1195 ACGT MboI 54 GATC ^GATC 559 GATC MboII 986 GAAGA GAAGA(8/7) 166 TCTTC NdeII 54 GATC ^GATC 559 GATC PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT] 1289 AGGACCT PstI 151 CTGCAG CTGCA^G 1387 CTGCAG Sau3AI 54 GATC ^GATC 559 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI BalI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflMI PleI PvuII RsaI SacI Sau96I ScrFI SfaNI SfcI StuI StyI TseI Tsp45I TspRI Van91I