################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 508 GAATTT [GA]^AATT[CT] AflII 253 CTTAAG C^TTAAG AflIII 1134 ACACGT A^C[GA][CT]GT AlwNI 1340 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 508 GAATTT [GA]^AATT[CT] BamHI 970 GGATCC G^GATCC BanI 30 GGTGCC G^G[CT][GA]CC BanII 1250 GGGCTC G[GA]GC[CT]^C BfaI 550 CTAG C^TAG BfrI 253 CTTAAG C^TTAAG BlpI 229 GCTCAGC GC^T[ACGT]AGC BmyI 1250 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 229 GCTCAGC GC^T[ACGT]AGC BsaWI 293 TCCGGT [AT]^CCGG[AT] BsiEI 48 CGGCCG CG[GA][CT]^CG BsmFI 342 GTCCC GGGAC(10/14) Bsp1286I 1250 GGGCTC G[AGT]GC[ACT]^C BspDI 205 ATCGAT AT^CGAT BspHI 1140 TCATGA T^CATGA BsrFI 665 ACCGGC [GA]^CCGG[CT] BsrI 684 CCAGT ACTGG(1/-1) BstYI 970 GGATCC [GA]^GATC[CT] CelII 229 GCTCAGC GC^T[ACGT]AGC CfoI 1260 GCGC GCG^C Cfr10I 665 ACCGGC [GA]^CCGG[CT] ClaI 205 ATCGAT AT^CGAT DraIII 14 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 48 CGGCCG C^GGCCG EclXI 48 CGGCCG C^GGCCG Eco52I 48 CGGCCG C^GGCCG HhaI 1260 GCGC GCG^C HinP1I 1260 GCGC G^CGC MaeI 550 CTAG C^TAG MflI 970 GGATCC [GA]^GATC[CT] PleI 1153 GAGTC GAGTC(4/5) PstI 66 CTGCAG CTGCA^G RcaI 1140 TCATGA T^CATGA SapI 690 GCTCTTC GCTCTTC(1/4) SfcI 66 CTGCAG C^T[GA][CT]AG XhoII 970 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 40 CGCG CG^CG 1259 CGCG AfaI 430 GTAC GT^AC 1240 GTAC AlwI 971 GATCC GGATC(4/5) 970 GGATC BalI 829 TGGCCA TGG^CCA 1349 TGGCCA BcnI 433 CCGGG CC^[GC]GG 1062 CCGGG BsaI 1090 GAGACC GGTCTC(1/5) 296 GGTCTC BseRI 707 CTCCTC GAGGAG(10/8) 1087 GAGGAG BsrDI 363 CATTGC GCAATG(2/0) 83 GCAATG BstUI 40 CGCG CG^CG 1259 CGCG DraII 340 GGGTCCC [GA]G^G[ACGT]CC[CT] 1289 AGGTCCT EarI 691 CTCTTC CTCTTC(1/4) 1108 GAAGAG EcoO109I 340 GGGTCCC [GA]G^G[ACGT]CC[CT] 1289 AGGTCCT FauI 38 CCCGC CCCGC(4/6) 266 GCGGG HphI 1357 GGTGA GGTGA(8/7) 1080 TCACC Ksp632I 691 CTCTTC CTCTTC(1/4) 1108 GAAGAG MaeII 821 ACGT A^CGT 1136 ACGT MaeIII 841 GTCAC ^GT[ACGT]AC 1417 GTCAC MluNI 829 TGGCCA TGG^CCA 1349 TGGCCA MscI 829 TGGCCA TGG^CCA 1349 TGGCCA MvnI 40 CGCG CG^CG 1259 CGCG NciI 433 CCGGG CC^[GC]GG 1062 CCGGG PpuMI 340 GGGTCCC [GA]G^G[AT]CC[CT] 1289 AGGTCCT RsaI 430 GTAC GT^AC 1240 GTAC SmlI 253 CTTAAG C^T[CT][GA]AG 310 CTCAAG TaqI 99 TCGA T^CGA 206 TCGA Tsp45I 841 GTCAC ^GT[GC]AC 1417 GTCAC TthHB8I 99 TCGA T^CGA 206 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AvaII BpmI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FokI HaeIII HapII HinfI HpaII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp509I TspRI