################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AccI AccIII AclI AcsI AflII AgeI AhdI AlwI ApoI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbvCI BciVI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Cfr10I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI TfiI Tru9I Tsp509I Van91I XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 982 GACGTC GACGT^C Acc65I 56 GGTACC G^GTACC AocI 938 CCTCAGG CC^T[ACGT]AGG Aor51HI 209 AGCGCT AGC^GCT Asp700I 369 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 56 GGTACC G^GTACC AspI 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 985 GTCTTC GAAGAC(2/6) BcgI 449 GCAAGAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 479 TGATCA T^GATCA BglI 493 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 985 GTCTTC GAAGAC(2/6) BsgI 1351 GTGCAG GTGCAG(16/14) BsiEI 1104 CGGCCG CG[GA][CT]^CG BsrBI 1237 GAGCGG CCGCTC(-3/-3) BsrDI 579 GCAATG GCAATG(2/0) BsrI 1194 CCAGT ACTGG(1/-1) Bsu36I 938 CCTCAGG CC^T[ACGT]AGG DsaI 488 CCGTGG C^C[GA][CT]GG EagI 1104 CGGCCG C^GGCCG EclXI 1104 CGGCCG C^GGCCG Eco47III 209 AGCGCT AGC^GCT Eco52I 1104 CGGCCG C^GGCCG Eco81I 938 CCTCAGG CC^T[ACGT]AGG EcoT14I 1214 CCAAGG C^C[AT][AT]GG FbaI 479 TGATCA T^GATCA HinfI 1303 GACTC G^A[ACGT]TC KpnI 56 GGTACC GGTAC^C NotI 1103 GCGGCCGC GC^GGCCGC PaeR7I 1399 CTCGAG C^TCGAG PflFI 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1303 GACTC GAGTC(4/5) SacI 754 GAGCTC GAGCT^C SapI 1167 GCTCTTC GCTCTTC(1/4) SbfI 281 CCTGCAGG CCTGCA^GG SfiI 492 GGCCTTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 281 CCTGCAGG CCTGCA^GG StyI 1214 CCAAGG C^C[AT][AT]GG Tth111I 346 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 324 TCTAGA T^CTAGA XhoI 1399 CTCGAG C^TCGAG XmnI 369 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 645 GGCGTC G[GA]^CG[CT]C 982 GACGTC Alw44I 401 GTGCAC G^TGCAC 1197 GTGCAC ApaI 500 GGGCCC GGGCC^C 945 GGGCCC ApaLI 401 GTGCAC G^TGCAC 1197 GTGCAC BanI 56 GGTACC G^G[CT][GA]CC 470 GGCACC BfaI 325 CTAG C^TAG 1126 CTAG BlpI 600 GCTCAGC GC^T[ACGT]AGC 1056 GCTGAGC BpmI 64 CTGGAG CTGGAG(16/14) 610 CTGGAG Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC 1056 GCTGAGC BsaHI 645 GGCGTC G[GA]^CG[CT]C 982 GACGTC BsmAI 74 GAGAC GTCTC(1/5) 1297 GAGAC BsmBI 74 GAGACG CGTCTC(1/5) 1297 GAGACG Bsp1407I 518 TGTACA T^GTACA 1010 TGTACA BspLU11I 1007 ACATGT A^CATGT 1070 ACATGT BsrGI 518 TGTACA T^GTACA 1010 TGTACA BssSI 142 CACGAG CACGAG(-5/-1) 1411 CTCGTG BstAPI 403 GCACGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1383 GCAGCGTCTGC BstXI 422 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1418 CCATCGGTCTGG CelII 600 GCTCAGC GC^T[ACGT]AGC 1056 GCTGAGC DpnII 480 GATC ^GATC 766 GATC DraIII 227 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 404 CACGTAGTG EarI 1168 CTCTTC CTCTTC(1/4) 1092 GAAGAG EcoNI 281 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 708 CCTCCGGGAGG Hin1I 645 GGCGTC G[GA]^CG[CT]C 982 GACGTC Ksp632I 1168 CTCTTC CTCTTC(1/4) 1092 GAAGAG MaeI 325 CTAG C^TAG 1126 CTAG MboI 480 GATC ^GATC 766 GATC NdeII 480 GATC ^GATC 766 GATC PpuMI 593 GGGACCT [GA]G^G[AT]CC[CT] 1403 AGGACCT Sau3AI 480 GATC ^GATC 766 GATC SmlI 1186 CTTGAG C^T[CT][GA]AG 1399 CTCGAG SspBI 518 TGTACA T^GTACA 1010 TGTACA StuI 860 AGGCCT AGG^CCT 1086 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwNI AspHI AvaI AvaII BalI BanII BbrPI BbvI BcnI BmyI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI PmaCI PmlI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I