(Linear) (Six-base) MAPSORT of: KIEE4108.seq Check: 8962 from: 1 to: 1434 KIEE4108.orf With 182 enzymes: SgfI * March 22, 2012 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 702 1434 Size: 702 732 AatII G_ACGT'C Cuts at: 0 986 1434 Size: 986 448 Acc65I G'GTAC_C Cuts at: 0 56 1434 Size: 56 1378 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1434 Size: 1111 323 AfeI AGC'GCT Cuts at: 0 211 1434 Size: 211 1223 AflIII A'CryG_T Cuts at: 0 661 1007 1070 1434 Size: 661 346 63 364 Fragments arranged by size: 661 364 346 63 AleI CACnn'nnGTG Cuts at: 0 664 994 1141 1434 Size: 664 330 147 293 Fragments arranged by size: 664 330 293 147 AlwNI CAG_nnn'CTG Cuts at: 0 516 1279 1389 1434 Size: 516 763 110 45 Fragments arranged by size: 763 516 110 45 ApaI G_GGCC'C Cuts at: 0 504 949 1434 Size: 504 445 485 Fragments arranged by size: 504 485 445 ApaLI G'TGCA_C Cuts at: 0 401 1197 1434 Size: 401 796 237 Fragments arranged by size: 796 401 237 AvaI C'yCGr_G Cuts at: 0 751 1036 1399 1434 Size: 751 285 363 35 Fragments arranged by size: 751 363 285 35 BanI G'GyrC_C Cuts at: 0 56 470 1434 Size: 56 414 964 Fragments arranged by size: 964 414 56 BanII G_rGCy'C Cuts at: 0 180 252 504 689 758 897 949 1434 Size: 180 72 252 185 69 139 52 485 Fragments arranged by size: 485 252 185 180 139 72 69 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 978 1434 Size: 978 456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 438 472 1434 Size: 438 34 962 Fragments arranged by size: 962 438 34 BclI T'GATC_A Cuts at: 0 479 1434 Size: 479 955 BglI GCCn_nnn'nGGC Cuts at: 0 499 1434 Size: 499 935 BlpI GC'TnA_GC Cuts at: 0 601 1057 1434 Size: 601 456 377 Fragments arranged by size: 601 456 377 Bme1580I G_kGCm'C Cuts at: 0 143 228 405 504 744 949 1201 1398 Size: 143 85 177 99 240 205 252 197 Cuts at: 1398 1434 Size: 36 Fragments arranged by size: 252 240 205 197 177 143 99 85 36 BmgBI CAC'GTC Cuts at: 0 78 348 1434 Size: 78 270 1086 Fragments arranged by size: 1086 270 78 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1320 1434 Size: 1320 114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 631 1434 Size: 85 546 803 Fragments arranged by size: 803 546 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1207 1434 Size: 1207 227 BsaAI yAC'GTr Cuts at: 0 406 662 996 1338 1434 Size: 406 256 334 342 96 Fragments arranged by size: 406 342 334 256 96 BsaHI Gr'CG_yC Cuts at: 0 646 983 1434 Size: 646 337 451 Fragments arranged by size: 646 451 337 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 604 634 1434 Size: 604 30 800 Fragments arranged by size: 800 604 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 940 1282 1387 1434 Size: 160 780 342 105 47 Fragments arranged by size: 780 342 160 105 47 BseYI C'CCAG_C Cuts at: 0 951 1434 Size: 951 483 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1372 1434 Size: 1372 62 BsiEI CG_ry'CG Cuts at: 0 1107 1434 Size: 1107 327 BsiHKAI G_wGCw'C Cuts at: 0 405 758 1001 1201 1434 Size: 405 353 243 200 233 Fragments arranged by size: 405 353 243 233 200 BsmBI CGTCTCn'nnnn_ Cuts at: 0 68 1291 1434 Size: 68 1223 143 Fragments arranged by size: 1223 143 68 Bsp1286I G_dGCh'C Cuts at: 0 143 180 228 252 405 504 689 744 Size: 143 37 48 24 153 99 185 55 Cuts at: 744 758 897 949 1001 1201 1398 1434 Size: 14 139 52 52 200 197 36 Fragments arranged by size: 200 197 185 153 143 139 99 55 52 52 48 37 36 24 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 68 258 605 702 1085 1415 1434 Size: 68 190 347 97 383 330 19 Fragments arranged by size: 383 347 330 190 97 68 19 BsrBI CCG'CTC Cuts at: 0 1239 1434 Size: 1239 195 BsrDI GCAATG_nn' Cuts at: 0 586 1434 Size: 586 848 BsrGI T'GTAC_A Cuts at: 0 518 1010 1434 Size: 518 492 424 Fragments arranged by size: 518 492 424 BssSI C'ACGA_G Cuts at: 0 142 1411 1434 Size: 142 1269 23 Fragments arranged by size: 1269 142 23 BstAPI GCAn_nnn'nTGC Cuts at: 0 409 1389 1434 Size: 409 980 45 Fragments arranged by size: 980 409 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 429 1425 1434 Size: 429 996 9 Fragments arranged by size: 996 429 9 Bsu36I CC'TnA_GG Cuts at: 0 939 1434 Size: 939 495 BtgI C'CryG_G Cuts at: 0 488 1434 Size: 488 946 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 370 1434 Size: 370 1064 BtsI GCAGTG_nn' Cuts at: 0 885 1434 Size: 885 549 DraIII CAC_nnn'GTG Cuts at: 0 232 409 1434 Size: 232 177 1025 Fragments arranged by size: 1025 232 177 EaeI y'GGCC_r Cuts at: 0 167 572 731 873 1104 1160 1211 1340 Size: 167 405 159 142 231 56 51 129 Cuts at: 1340 1434 Size: 94 Fragments arranged by size: 405 231 167 159 142 129 94 56 51 EagI C'GGCC_G Cuts at: 0 1104 1434 Size: 1104 330 EarI CTCTTCn'nnn_ Cuts at: 0 1087 1174 1434 Size: 1087 87 260 Fragments arranged by size: 1087 260 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 232 1162 1434 Size: 232 930 272 Fragments arranged by size: 930 272 232 EcoICRI GAG'CTC Cuts at: 0 756 1434 Size: 756 678 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 631 1111 1434 Size: 85 546 480 323 Fragments arranged by size: 546 480 323 85 EcoNI CCTnn'n_nnAGG Cuts at: 0 285 712 1434 Size: 285 427 722 Fragments arranged by size: 722 427 285 EcoO109I rG'GnC_Cy Cuts at: 0 500 501 594 945 946 1404 1434 Size: 500 1 93 351 1 458 30 Fragments arranged by size: 500 458 351 93 30 1 1 HaeII r_GCGC'y Cuts at: 0 213 570 727 1367 1434 Size: 213 357 157 640 67 Fragments arranged by size: 640 357 213 157 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 616 648 1434 Size: 616 32 786 Fragments arranged by size: 786 616 32 KpnI G_GTAC'C Cuts at: 0 60 1434 Size: 60 1374 MscI TGG'CCA Cuts at: 0 169 574 1162 1213 1342 1434 Size: 169 405 588 51 129 92 Fragments arranged by size: 588 405 169 129 92 51 MslI CAynn'nnrTG Cuts at: 0 554 664 994 1141 1434 Size: 554 110 330 147 293 Fragments arranged by size: 554 330 293 147 110 MspA1I CmG'CkG Cuts at: 0 26 182 318 588 809 1276 1314 1434 Size: 26 156 136 270 221 467 38 120 Fragments arranged by size: 467 270 221 156 136 120 38 26 NotI GC'GGCC_GC Cuts at: 0 1104 1434 Size: 1104 330 NspI r_CATG'y Cuts at: 0 429 553 1011 1074 1434 Size: 429 124 458 63 360 Fragments arranged by size: 458 429 360 124 63 PciI A'CATG_T Cuts at: 0 1007 1070 1434 Size: 1007 63 364 Fragments arranged by size: 1007 364 63 PfoI T'CCnGG_A Cuts at: 0 710 1434 Size: 710 724 PmlI CAC'GTG Cuts at: 0 662 996 1338 1434 Size: 662 334 342 96 Fragments arranged by size: 662 342 334 96 PpuMI rG'GwC_Cy Cuts at: 0 594 1404 1434 Size: 594 810 30 Fragments arranged by size: 810 594 30 PspOMI G'GGCC_C Cuts at: 0 500 945 1434 Size: 500 445 489 Fragments arranged by size: 500 489 445 PspXI vC'TCGA_Gb Cuts at: 0 1399 1434 Size: 1399 35 PstI C_TGCA'G Cuts at: 0 31 286 512 699 844 1434 Size: 31 255 226 187 145 590 Fragments arranged by size: 590 255 226 187 145 31 PvuII CAG'CTG Cuts at: 0 26 588 809 1434 Size: 26 562 221 625 Fragments arranged by size: 625 562 221 26 SacI G_AGCT'C Cuts at: 0 758 1434 Size: 758 676 SapI GCTCTTCn'nnn_ Cuts at: 0 1174 1434 Size: 1174 260 SbfI CC_TGCA'GG Cuts at: 0 286 1434 Size: 286 1148 SfcI C'TryA_G Cuts at: 0 27 282 508 695 840 1359 1434 Size: 27 255 226 187 145 519 75 Fragments arranged by size: 519 255 226 187 145 75 27 SfiI GGCCn_nnn'nGGCC Cuts at: 0 499 1434 Size: 499 935 SmlI C'TyrA_G Cuts at: 0 1186 1399 1434 Size: 1186 213 35 Fragments arranged by size: 1186 213 35 StuI AGG'CCT Cuts at: 0 862 1088 1434 Size: 862 226 346 Fragments arranged by size: 862 346 226 StyI C'CwwG_G Cuts at: 0 1214 1434 Size: 1214 220 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 388 488 1135 1411 1434 Size: 388 100 647 276 23 Fragments arranged by size: 647 388 276 100 23 TatI w'GTAC_w Cuts at: 0 119 360 518 1010 1205 1434 Size: 119 241 158 492 195 229 Fragments arranged by size: 492 241 229 195 158 119 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 604 636 1434 Size: 604 32 798 Fragments arranged by size: 798 604 32 Tth111I GACn'n_nGTC Cuts at: 0 349 1434 Size: 349 1085 XbaI T'CTAG_A Cuts at: 0 324 1434 Size: 324 1110 XhoI C'TCGA_G Cuts at: 0 1399 1434 Size: 1399 35 XmnI GAAnn'nnTTC Cuts at: 0 373 1434 Size: 373 1061 ZraI GAC'GTC Cuts at: 0 984 1434 Size: 984 450 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MscI MslI MspA1I NotI NspI PciI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI SfcI SfiI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI Enzymes that do not cut: AccI AclI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmrI BmtI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrFI BssHII BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XcmI XmaI