(Circular) (Six-base) MAPSORT of: pFN21AE0695.seq Check: 4964 from: 1 to: 5557 pFN21AE0695 5557 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2017 2878 2017 Size: 861 4696 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2495 3620 5004 5272 1632 Size: 758 105 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 758 268 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 4456 5433 1203 Size: 3253 977 1327 Fragments arranged by size: 3253 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2819 2851 2819 Size: 32 5525 AlwNI CAG_nnn'CTG Cuts at: 2653 3300 4872 2653 Size: 647 1572 3338 Fragments arranged by size: 3338 1572 647 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2882 1870 Size: 73 939 4545 Fragments arranged by size: 4545 939 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2291 2452 2878 4333 Size: 325 217 627 230 274 161 426 1455 Cuts at: 4333 618 Size: 1842 Fragments arranged by size: 1842 1455 627 426 325 274 230 217 161 BanII G_rGCy'C Cuts at: 1391 2075 2233 2876 1391 Size: 684 158 643 4072 Fragments arranged by size: 4072 684 643 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 2606 2715 928 Size: 971 247 460 109 3770 Fragments arranged by size: 3770 971 460 247 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BfrBI ATG'CAT Cuts at: 2642 2642 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 4168 136 Size: 107 122 71 631 605 565 1931 1525 Fragments arranged by size: 1931 1525 631 605 565 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2069 2974 2069 Size: 905 4652 Bme1580I G_kGCm'C Cuts at: 1790 3618 4774 1790 Size: 1828 1156 2573 Fragments arranged by size: 2573 1828 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2697 3288 4246 Size: 416 53 83 399 24 1280 591 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1280 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 4217 1239 Size: 1017 1961 2579 Fragments arranged by size: 2579 1961 1017 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2075 2247 3990 4388 4662 4809 5220 2075 Size: 172 1743 398 274 147 411 2412 Fragments arranged by size: 2412 1743 411 398 274 172 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2786 2816 2819 2849 1178 Size: 30 165 30 1383 30 3 30 3886 Fragments arranged by size: 3886 1383 165 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2561 2807 2840 1186 Size: 1375 246 33 3903 Fragments arranged by size: 3903 1375 246 33 BseYI C'CCAG_C Cuts at: 4760 5256 4760 Size: 496 5061 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4984 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2248 3007 3768 4796 1540 Size: 275 186 87 160 759 761 1028 2301 Fragments arranged by size: 2301 1028 761 759 275 186 160 87 BsiHKAI G_wGCw'C Cuts at: 2075 3618 3703 4774 2075 Size: 1543 85 1071 2858 Fragments arranged by size: 2858 1543 1071 85 BsmI GAATG_Cn' Cuts at: 2642 3068 3161 2642 Size: 426 93 5038 Fragments arranged by size: 5038 426 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2233 2876 3618 3703 4774 1391 Size: 399 285 158 643 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 399 285 158 85 BspEI T'CCGG_A Cuts at: 2075 5220 2075 Size: 3145 2412 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2912 844 Size: 2068 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3444 1394 Size: 429 1621 3507 Fragments arranged by size: 3507 1621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4201 4388 1700 Size: 110 2391 187 2869 Fragments arranged by size: 2869 2391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 2219 794 Size: 1425 4132 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552 Size: 915 367 385 17 1452 86 12 346 Cuts at: 5552 1972 Size: 1977 Fragments arranged by size: 1977 1452 915 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 513 Size: 552 939 78 3988 Fragments arranged by size: 3988 939 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2175 3073 3867 3887 2175 Size: 898 794 20 3845 Fragments arranged by size: 3845 898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2785 3004 Size: 54 1425 50 275 18 255 700 219 Cuts at: 3004 3893 5522 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 700 275 255 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 3004 1537 Size: 275 273 919 4090 Fragments arranged by size: 4090 919 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 3487 1353 Size: 1473 661 3423 Fragments arranged by size: 3423 1473 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4144 4518 4664 387 Size: 1064 138 435 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2073 2073 Size: 5557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2390 2495 3620 4217 5004 5272 Size: 393 624 134 105 1125 597 787 268 Cuts at: 5272 1239 Size: 1524 Fragments arranged by size: 1524 1125 787 624 597 393 268 134 105 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 4704 1678 Size: 3026 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 2402 2612 748 Size: 1654 210 3693 Fragments arranged by size: 3693 1654 210 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2021 2882 2021 Size: 861 4696 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2787 10 Size: 54 1425 343 955 2780 Fragments arranged by size: 2780 1425 955 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2973 3650 4798 5043 1606 Size: 1367 677 1148 245 2120 Fragments arranged by size: 2120 1367 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NsiI A_TGCA'T Cuts at: 2644 2644 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2244 2915 4460 1132 Size: 97 1015 671 1545 2229 Fragments arranged by size: 2229 1545 1015 671 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 4312 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2819 2851 3626 3658 5139 5171 2819 Size: 32 775 32 1481 32 3205 Fragments arranged by size: 3205 1481 775 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2170 2909 830 Size: 1340 739 3478 Fragments arranged by size: 3478 1340 739 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SacI G_AGCT'C Cuts at: 2075 2075 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2166 2905 4040 4721 4912 826 Size: 219 1121 739 1135 681 191 1471 Fragments arranged by size: 1471 1135 1121 739 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3661 4562 4824 820 Size: 197 926 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2244 2915 1132 Size: 1112 671 3774 Fragments arranged by size: 3774 1112 671 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2996 513 Size: 552 939 992 3074 Fragments arranged by size: 3074 992 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2370 3584 3601 3754 3939 4384 325 Size: 1255 790 1214 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1214 790 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 3803 5515 Size: 274 80 33 51 1443 717 1109 1712 Cuts at: 5515 96 Size: 138 Fragments arranged by size: 1712 1443 1109 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2668 2893 2668 Size: 225 5332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 2480 3686 2480 Size: 1206 4351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI