Restriction map for
pFN21AE0689
(Circular) (Six-base) MAPSORT of: pFN21AE0689.seq Check: 865 from: 1 to: 5545
pFN21AE0689 5545 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2160 3608 4992 5260 1632
Size: 528 1448 1384 268 1917
Fragments arranged by size:
1917 1448 1384 528 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 2602 4274 2602
Size: 1672 3873
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5545
AlwNI CAG_nnn'CTG
Cuts at: 3288 4860 3288
Size: 1572 3973
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2282 2297 2854 3080 3232 5341 1493
Size: 789 15 557 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 557 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 1870
Size: 73 927 4545
Fragments arranged by size:
4545 927 73
BamHI G'GATC_C
Cuts at: 2047 2622 2875 3242 2047
Size: 575 253 367 4350
Fragments arranged by size:
4350 575 367 253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 2866 4321 618
Size: 325 217 627 711 368 1455 1842
Fragments arranged by size:
1842 1455 711 627 368 325 217
BanII G_rGCy'C
Cuts at: 1391 2864 1391
Size: 1473 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 1903
Size: 34 1799 34 3678
Fragments arranged by size:
3678 1799 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4156 136
Size: 107 122 71 631 605 2484 1525
Fragments arranged by size:
2484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2056 5540 2056
Size: 3484 2061
BlpI GC'TnA_GC
Cuts at: 2085 2962 2085
Size: 877 4668
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2501 3606 4762 1790
Size: 691 20 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 691 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 4205 1239
Size: 839 2127 2579
Fragments arranged by size:
2579 2127 839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 4208 882
Size: 617 693 2016 2219
Fragments arranged by size:
2219 2016 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3734 4369 275
Size: 53 83 186 936 1819 382 635 1451
Fragments arranged by size:
1819 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3978 4376 4650 4797 5208 3978
Size: 398 274 147 411 4315
Fragments arranged by size:
4315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2520 2550 1178
Size: 30 165 30 1117 30 4173
Fragments arranged by size:
4173 1117 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4590
BseYI C'CCAG_C
Cuts at: 4748 5244 4748
Size: 496 5049
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4156
Fragments arranged by size:
4156 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2831 2995 3756 4784 1540
Size: 275 186 830 164 761 1028 2301
Fragments arranged by size:
2301 1028 830 761 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 2042 3606 3691 4762 2042
Size: 1564 85 1071 2825
Fragments arranged by size:
2825 1564 1071 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2481 2501 2864 3606 3691 4762
Size: 399 252 439 20 363 742 85 1071
Cuts at: 4762 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 439 399 363 252 85
20
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 844
Size: 2056 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 2451 4040 4214 65
Size: 1183 952 251 1589 174 1396
Fragments arranged by size:
1589 1396 1183 952 251 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 2650 96
Size: 2554 2991
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 2510 1184
Size: 1326 4219
BstYI r'GATC_y
Cuts at: 1972 2047 2056 2069 2622 2875 3242 3627 3644
Size: 75 9 13 553 253 367 385 17
Cuts at: 3644 5096 5182 5194 5540 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 553 385 367 346 253 86
75 17 13 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4054
Fragments arranged by size:
4054 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 3061 3855 3875 3061
Size: 794 20 4731
Fragments arranged by size:
4731 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2382 2417 529
Size: 35 1818 35 3657
Fragments arranged by size:
3657 1818 35 35
DraI TTT'AAA
Cuts at: 2281 2842 2849 2281
Size: 561 7 4977
Fragments arranged by size:
4977 561 7
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510
Size: 54 1425 50 275 18 1162 889 1629
Cuts at: 5510 8
Size: 43
Fragments arranged by size:
1629 1425 1162 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 3475 1353
Size: 798 1324 3423
Fragments arranged by size:
3423 1324 798
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4132 4506 4652 387
Size: 1064 138 2543 374 146 1280
Fragments arranged by size:
2543 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2160 3608 4205 4992 5260 1239
Size: 393 446 82 1448 597 787 268 1524
Fragments arranged by size:
1524 1448 787 597 446 393 268 82
EcoRI G'AATT_C
Cuts at: 2297 2854 3232 2297
Size: 557 378 4610
Fragments arranged by size:
4610 557 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2489 2489
Size: 5545
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 4692 1678
Size: 3014 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2350 2382 2520 2552 2588
Size: 32 135 32 778 32 138 32 36
Cuts at: 2588 2620 2701 2733 2809 2841 4186 4218 4260
Size: 32 81 32 76 32 1345 32 42
Cuts at: 4260 4292 1373
Size: 32 2626
Fragments arranged by size:
2626 1345 778 138 135 81 76 42
36 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3723
Fragments arranged by size:
3723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2369 2444 2574 3503 3862 4021
Size: 648 1087 116 75 130 929 359 159
Cuts at: 4021 518
Size: 2042
Fragments arranged by size:
2042 1087 929 648 359 159 130 116
75
MspA1I CmG'CkG
Cuts at: 1606 2648 2961 3638 4786 5031 1606
Size: 1042 313 677 1148 245 2120
Fragments arranged by size:
2120 1148 1042 677 313 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4054
Fragments arranged by size:
4054 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5545
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 1108
Size: 993 4552
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 5545
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2897 830
Size: 2067 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2648 2648
Size: 5545
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SapI GCTCTTCn'nnn_
Cuts at: 2151 2151
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2893 4028 4709 4900 826
Size: 219 1848 1135 681 191 1471
Fragments arranged by size:
1848 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2872 1872
Size: 1000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3649 4550 4812 820
Size: 197 926 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 2532 1517
Size: 1015 4530
StyI C'CwwG_G
Cuts at: 513 1065 2004 2803 2984 513
Size: 552 939 799 181 3074
Fragments arranged by size:
3074 939 799 552 181
SwaI ATTT'AAAT
Cuts at: 2281 2281
Size: 5545
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 2817 3572 3589 3742 3927 4372
Size: 1255 477 760 755 17 153 185 445
Cuts at: 4372 325
Size: 1498
Fragments arranged by size:
1498 1255 760 755 477 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 2650 3791
Size: 274 80 33 51 1443 234 439 1141
Cuts at: 3791 5503 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1141 439 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 2090 2122 5505 5537 2032
Size: 32 26 32 3383 32 2040
Fragments arranged by size:
3383 2040 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2023 2881 2023
Size: 858 4687
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5545
XmaI C'CCGG_G
Cuts at: 1870 2870 1870
Size: 1000 4545
XmnI GAAnn'nnTTC
Cuts at: 2145 2588 2616 3674 2145
Size: 443 28 1058 4016
Fragments arranged by size:
4016 1058 443 28
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI XcmI