(Circular) (Six-base) MAPSORT of: pFN21AE0688.seq Check: 6446 from: 1 to: 5908 pFN21AE0688 5908 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2195 2261 3971 5355 5623 1632 Size: 446 117 66 1710 1384 268 1917 Fragments arranged by size: 1917 1710 1384 446 268 117 66 AflII C'TTAA_G Cuts at: 820 1017 2903 820 Size: 197 1886 3825 Fragments arranged by size: 3825 1886 197 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AlwNI CAG_nnn'CTG Cuts at: 2468 2764 2785 3651 5223 2468 Size: 296 21 866 1572 3153 Fragments arranged by size: 3153 1572 866 296 21 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3033 3077 3176 3217 3443 3595 5704 1493 Size: 1540 44 99 41 226 152 2109 1697 Fragments arranged by size: 2109 1697 1540 226 152 99 44 41 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2571 3233 1870 Size: 73 628 662 4545 Fragments arranged by size: 4545 662 628 73 AvrII C'CTAG_G Cuts at: 2023 2023 Size: 5908 BamHI G'GATC_C Cuts at: 2245 3238 3605 2245 Size: 993 367 4548 Fragments arranged by size: 4548 993 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3041 3229 4684 618 Size: 325 217 627 1254 188 1455 1842 Fragments arranged by size: 1842 1455 1254 627 325 217 188 BanII G_rGCy'C Cuts at: 1391 3227 1391 Size: 1836 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2607 2816 2970 928 Size: 971 708 209 154 3866 Fragments arranged by size: 3866 971 708 209 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2818 2852 4099 4133 1903 Size: 34 881 34 1247 34 3678 Fragments arranged by size: 3678 1247 881 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BfrBI ATG'CAT Cuts at: 2864 2864 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2666 5903 2666 Size: 3237 2671 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 2714 3046 3969 5125 1790 Size: 924 332 923 1156 2573 Fragments arranged by size: 2573 1156 924 923 332 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 2250 2633 Size: 416 53 83 399 24 739 94 383 Cuts at: 2633 3639 4597 442 Size: 1006 958 1753 Fragments arranged by size: 1753 1006 958 739 416 399 383 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4568 1239 Size: 3329 2579 Bpu10I CC'TnA_GC Cuts at: 2290 2786 2290 Size: 496 5412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 4033 4898 5196 2581 Size: 1452 865 298 3293 Fragments arranged by size: 3293 1452 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2027 2446 4571 882 Size: 617 528 419 2125 2219 Fragments arranged by size: 2219 2125 617 528 419 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 2883 5902 2883 Size: 3019 2889 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2847 3715 4097 4732 Size: 53 83 186 936 1314 868 382 635 Cuts at: 4732 275 Size: 1451 Fragments arranged by size: 1451 1314 936 868 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2675 4341 4739 5013 5160 5571 2675 Size: 1666 398 274 147 411 3012 Fragments arranged by size: 3012 1666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5683 Fragments arranged by size: 5683 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 2512 1186 Size: 993 333 4582 Fragments arranged by size: 4582 993 333 BseYI C'CCAG_C Cuts at: 2234 5111 5607 2234 Size: 2877 496 2535 Fragments arranged by size: 2877 2535 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 4119 5147 1540 Size: 275 186 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2792 3969 4054 5125 2792 Size: 1177 85 1071 3575 Fragments arranged by size: 3575 1177 1071 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2714 2792 3046 3227 3969 4054 5125 Size: 399 924 78 254 181 742 85 1071 Cuts at: 5125 1391 Size: 2174 Fragments arranged by size: 2174 1071 924 742 399 254 181 85 78 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2526 4403 4577 65 Size: 1183 1278 1877 174 1396 Fragments arranged by size: 1877 1396 1278 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 4552 4739 1700 Size: 110 389 2353 187 2869 Fragments arranged by size: 2869 2353 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5908 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2245 2458 2666 3238 3605 3990 4007 5459 Size: 273 213 208 572 367 385 17 1452 Cuts at: 5459 5545 5557 5903 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 572 385 367 346 273 213 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5356 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3424 4218 4238 2790 Size: 634 794 20 4460 Fragments arranged by size: 4460 794 634 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873 Size: 54 1425 50 275 18 1525 889 1629 Cuts at: 5873 8 Size: 43 Fragments arranged by size: 1629 1525 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2583 3838 1353 Size: 1230 1255 3423 Fragments arranged by size: 3423 1255 1230 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2532 4495 4869 5015 387 Size: 1064 138 943 1963 374 146 1280 Fragments arranged by size: 1963 1280 1064 943 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2195 2261 3971 4568 5355 5623 Size: 393 446 117 66 1710 597 787 268 Cuts at: 5623 1239 Size: 1524 Fragments arranged by size: 1710 1524 787 597 446 393 268 117 66 EcoO109I rG'GnC_Cy Cuts at: 2782 2782 Size: 5908 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 5908 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2347 5055 1678 Size: 669 2708 2531 Fragments arranged by size: 2708 2531 669 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 4549 4581 4623 Size: 32 135 32 871 32 2074 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 2074 871 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3115 3252 3498 669 Size: 988 281 1177 137 246 3079 Fragments arranged by size: 3079 1177 988 281 246 137 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2696 2992 5022 5206 1305 Size: 1391 296 2030 184 2007 Fragments arranged by size: 2030 2007 1391 296 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4225 4384 518 Size: 648 2700 359 159 2042 Fragments arranged by size: 2700 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 2371 2890 3324 4001 5149 5394 1606 Size: 628 137 519 434 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 628 519 434 245 137 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5356 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NsiI A_TGCA'T Cuts at: 2866 2866 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2228 3159 3266 4811 1132 Size: 97 999 931 107 1545 2229 Fragments arranged by size: 2229 1545 999 931 107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5908 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 2764 1108 Size: 1656 4252 PfoI T'CCnGG_A Cuts at: 2748 2748 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PstI C_TGCA'G Cuts at: 830 2085 2272 3260 830 Size: 1255 187 988 3478 Fragments arranged by size: 3478 1255 988 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2234 2234 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 2081 2268 3256 4391 5072 5263 826 Size: 219 1036 187 988 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 988 681 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2596 2903 4012 4913 5175 820 Size: 197 926 653 307 1109 901 262 1553 Fragments arranged by size: 1553 1109 926 901 653 307 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2023 2982 3347 513 Size: 552 958 959 365 3074 Fragments arranged by size: 3074 959 958 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 2677 3935 3952 4105 4290 4735 Size: 1255 971 126 1258 17 153 185 445 Cuts at: 4735 325 Size: 1498 Fragments arranged by size: 1498 1258 1255 971 445 185 153 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2147 4154 5866 Size: 274 80 33 51 1443 170 2007 1712 Cuts at: 5866 96 Size: 138 Fragments arranged by size: 2007 1712 1443 274 170 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 5868 5900 3006 Size: 32 2830 32 3014 Fragments arranged by size: 3014 2830 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI