(Circular) (Six-base) MAPSORT of: pFN21AE0683.seq Check: 4516 from: 1 to: 5527 pFN21AE0683 5527 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2789 3590 4974 5242 1632 Size: 713 444 801 1384 268 1917 Fragments arranged by size: 1917 1384 801 713 444 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 2060 1166 Size: 894 4633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 2323 3270 4842 2323 Size: 947 1572 3008 Fragments arranged by size: 3008 1572 947 ApaI G_GGCC'C Cuts at: 2110 2110 Size: 5527 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2109 2852 1870 Size: 73 115 51 743 4545 Fragments arranged by size: 4545 743 115 73 51 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 2416 2538 2848 4303 Size: 325 217 627 397 232 122 310 1455 Cuts at: 4303 618 Size: 1842 Fragments arranged by size: 1842 1455 627 397 325 310 232 217 122 BanII G_rGCy'C Cuts at: 1391 2110 2779 2846 1391 Size: 719 669 67 4072 Fragments arranged by size: 4072 719 669 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3718 3752 1903 Size: 34 1781 34 3678 Fragments arranged by size: 3678 1781 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2437 3419 4629 2437 Size: 982 1210 3335 Fragments arranged by size: 3335 1210 982 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BfrBI ATG'CAT Cuts at: 2683 2683 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2251 4138 136 Size: 107 122 71 631 605 579 1887 1525 Fragments arranged by size: 1887 1525 631 605 579 122 107 71 BglII A'GATC_T Cuts at: 2155 2572 5522 2155 Size: 417 2950 2160 Fragments arranged by size: 2950 2160 417 BlpI GC'TnA_GC Cuts at: 2944 2944 Size: 5527 Bme1580I G_kGCm'C Cuts at: 1790 2110 2187 2421 2769 3588 4744 1790 Size: 320 77 234 348 819 1156 2573 Fragments arranged by size: 2573 1156 819 348 320 234 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2216 3258 4216 Size: 416 53 83 399 24 799 1042 958 Cuts at: 4216 442 Size: 1753 Fragments arranged by size: 1753 1042 958 799 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4187 1239 Size: 2948 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 2763 3652 4517 4815 2354 Size: 409 889 865 298 3066 Fragments arranged by size: 3066 889 865 409 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 3318 493 Size: 711 1560 554 2702 Fragments arranged by size: 2702 1560 711 554 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3334 3716 4351 275 Size: 53 83 186 936 1801 382 635 1451 Fragments arranged by size: 1801 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5302 Fragments arranged by size: 5302 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 1186 Size: 1294 4233 BseYI C'CCAG_C Cuts at: 2040 4730 5226 2040 Size: 2690 496 2341 Fragments arranged by size: 2690 2341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 1163 Size: 573 444 4510 Fragments arranged by size: 4510 573 444 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2779 3588 3673 4744 2779 Size: 809 85 1071 3562 Fragments arranged by size: 3562 1071 809 85 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 BsmBI CGTCTCn'nnnn_ Cuts at: 2630 2630 Size: 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2187 2421 2769 2779 2846 3588 Size: 399 320 77 234 348 10 67 742 Cuts at: 3588 3673 4744 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 320 234 85 77 67 10 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2882 844 Size: 2038 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3414 1394 Size: 429 1591 3507 Fragments arranged by size: 3507 1591 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4022 4196 65 Size: 1183 2774 174 1396 Fragments arranged by size: 2774 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4171 4358 1700 Size: 110 2361 187 2869 Fragments arranged by size: 2869 2361 187 110 BsrGI T'GTAC_A Cuts at: 96 2503 96 Size: 2407 3120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 2339 3587 4599 2339 Size: 1248 1012 3267 Fragments arranged by size: 3267 1248 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5527 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2155 2572 2857 3224 3609 3626 5078 5164 Size: 183 417 285 367 385 17 1452 86 Cuts at: 5164 5176 5522 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 417 385 367 346 285 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2610 2610 Size: 5527 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4036 Fragments arranged by size: 4036 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 2412 2462 3043 3837 3857 2412 Size: 50 581 794 20 4082 Fragments arranged by size: 4082 794 581 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2065 2100 529 Size: 35 1501 35 3956 Fragments arranged by size: 3956 1501 35 35 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2252 2974 3863 Size: 54 1425 50 275 18 422 722 889 Cuts at: 3863 5492 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 722 422 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2808 3457 1353 Size: 761 694 649 3423 Fragments arranged by size: 3423 761 694 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2777 2777 Size: 5527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2789 3590 4187 4974 5242 1239 Size: 393 713 444 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 713 597 444 393 268 EcoO109I rG'GnC_Cy Cuts at: 2099 2431 2099 Size: 332 5195 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 4674 1678 Size: 2996 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2479 2511 2564 2596 4168 Size: 32 135 32 907 32 53 32 1572 Cuts at: 4168 4200 4242 4274 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1572 907 135 53 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2254 10 Size: 54 1425 343 422 3283 Fragments arranged by size: 3283 1425 422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2688 3485 3844 4003 518 Size: 648 894 628 797 359 159 2042 Fragments arranged by size: 2042 894 797 648 628 359 159 MspA1I CmG'CkG Cuts at: 1606 2323 2523 2943 3620 4768 5013 1606 Size: 717 200 420 677 1148 245 2120 Fragments arranged by size: 2120 1148 717 677 420 245 200 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4036 Fragments arranged by size: 4036 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NsiI A_TGCA'T Cuts at: 2685 2685 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2885 4430 1132 Size: 97 1656 1545 2229 Fragments arranged by size: 2229 1656 1545 97 PasI CC'CwG_GG Cuts at: 1348 2103 2770 1348 Size: 755 667 4105 Fragments arranged by size: 4105 755 667 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 1108 Size: 1563 3964 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PmlI CAC'GTG Cuts at: 2764 2764 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2431 2099 Size: 332 5195 PshAI GACnn'nnGTC Cuts at: 1613 2748 1613 Size: 1135 4392 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PspOMI G'GGCC_C Cuts at: 2106 2106 Size: 5527 PstI C_TGCA'G Cuts at: 830 2567 2879 830 Size: 1737 312 3478 Fragments arranged by size: 3478 1737 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 PvuII CAG'CTG Cuts at: 2323 2523 2323 Size: 200 5327 SacI G_AGCT'C Cuts at: 2779 2779 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SanDI GG'GwC_CC Cuts at: 2099 2099 Size: 5527 SapI GCTCTTCn'nnn_ Cuts at: 2114 2114 Size: 5527 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 2226 3775 5487 2226 Size: 1549 1712 2266 Fragments arranged by size: 2266 1712 1549 SfcI C'TryA_G Cuts at: 826 1045 2435 2563 2875 4010 4691 4882 826 Size: 219 1390 128 312 1135 681 191 1471 Fragments arranged by size: 1471 1390 1135 681 312 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2060 2854 1872 Size: 188 794 4545 Fragments arranged by size: 4545 794 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 2778 3631 4532 4794 820 Size: 197 926 426 409 853 901 262 1553 Fragments arranged by size: 1553 926 901 853 426 409 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 2707 3451 5 Size: 47 2655 744 2081 Fragments arranged by size: 2655 2081 744 47 StuI AGG'CCT Cuts at: 1517 2337 1517 Size: 820 4707 StyI C'CwwG_G Cuts at: 513 1065 2004 2421 2966 513 Size: 552 939 417 545 3074 Fragments arranged by size: 3074 939 552 545 417 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2402 2804 3554 3571 3724 3909 Size: 1255 474 348 402 750 17 153 185 Cuts at: 3909 4354 325 Size: 445 1498 Fragments arranged by size: 1498 1255 750 474 445 402 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 2503 2577 Size: 274 80 33 51 1443 247 279 74 Cuts at: 2577 2808 3773 5485 96 Size: 231 965 1712 138 Fragments arranged by size: 1712 1443 965 279 274 247 231 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 5487 5519 2189 Size: 32 3266 32 2197 Fragments arranged by size: 3266 2197 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5527 XbaI T'CTAG_A Cuts at: 2713 2863 2713 Size: 150 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2058 2852 1870 Size: 188 794 4545 Fragments arranged by size: 4545 794 188 XmnI GAAnn'nnTTC Cuts at: 3656 3656 Size: 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI