(Circular) (Six-base) MAPSORT of: pFN21AE0676.seq Check: 7603 from: 1 to: 5893 pFN21AE0676 5893 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2356 3236 1937 Size: 419 880 4594 Fragments arranged by size: 4594 880 419 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2339 2402 3956 5340 5608 1632 Size: 531 176 63 1554 1384 268 1917 Fragments arranged by size: 1917 1554 1384 531 268 176 63 AflII C'TTAA_G Cuts at: 820 1017 2479 820 Size: 197 1462 4234 Fragments arranged by size: 4234 1462 197 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 2706 1166 Size: 1540 4353 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 3636 5208 3636 Size: 1572 4321 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2173 2289 2484 2681 2959 3202 3428 3580 Size: 680 116 195 197 278 243 226 152 Cuts at: 3580 5689 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 680 278 243 226 197 195 152 116 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3218 1870 Size: 73 1275 4545 Fragments arranged by size: 4545 1275 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3214 4669 618 Size: 325 217 627 1427 1455 1842 Fragments arranged by size: 1842 1455 1427 627 325 217 BanII G_rGCy'C Cuts at: 1391 2045 3212 1391 Size: 654 1167 4072 Fragments arranged by size: 4072 1167 654 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 2655 928 Size: 971 142 614 4166 Fragments arranged by size: 4166 971 614 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4084 4118 1903 Size: 34 50 34 2063 34 3678 Fragments arranged by size: 3678 2063 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 4504 136 Size: 107 122 71 631 605 470 2362 1525 Fragments arranged by size: 2362 1525 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 2474 3310 2474 Size: 836 5057 Bme1580I G_kGCm'C Cuts at: 1790 3954 5110 1790 Size: 2164 1156 2573 Fragments arranged by size: 2573 2164 1156 BmgBI CAC'GTC Cuts at: 1318 2726 1318 Size: 1408 4485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2220 3624 4582 Size: 416 53 83 399 24 803 1404 958 Cuts at: 4582 442 Size: 1753 Fragments arranged by size: 1753 1404 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4553 1239 Size: 3314 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2010 4018 4883 5181 2010 Size: 2008 865 298 2722 Fragments arranged by size: 2722 2008 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2966 3684 493 Size: 711 1762 718 2702 Fragments arranged by size: 2702 1762 718 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 4082 4717 275 Size: 53 83 186 936 2167 382 635 1451 Fragments arranged by size: 2167 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4326 4724 4998 5145 5556 4326 Size: 398 274 147 411 4663 Fragments arranged by size: 4663 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5668 Fragments arranged by size: 5668 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3161 1186 Size: 1975 3918 BseYI C'CCAG_C Cuts at: 5096 5592 5096 Size: 496 5397 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 3954 4039 5110 2045 Size: 1909 85 1071 2828 Fragments arranged by size: 2828 1909 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 3212 3954 4039 5110 1391 Size: 399 255 1167 742 85 1071 2174 Fragments arranged by size: 2174 1167 1071 742 399 255 85 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4388 4562 65 Size: 1183 3140 174 1396 Fragments arranged by size: 3140 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 2010 2510 2686 3953 4965 2010 Size: 500 176 1267 1012 2938 Fragments arranged by size: 2938 1267 1012 500 176 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2911 2911 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2803 3223 3590 3975 3992 5444 5530 5542 Size: 831 420 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 831 420 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 2200 3409 4203 4223 2200 Size: 1209 794 20 3870 Fragments arranged by size: 3870 1209 794 20 ClaI AT'CG_AT Cuts at: 1554 2694 1554 Size: 1140 4753 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DraIII CAC_nnn'GTG Cuts at: 2207 2207 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 2773 3340 Size: 54 1425 50 275 18 304 639 567 Cuts at: 3340 4229 5858 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 639 567 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3823 1353 Size: 2470 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2043 2043 Size: 5893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2339 2402 3956 4553 5340 5608 Size: 393 531 176 63 1554 597 787 268 Cuts at: 5608 1239 Size: 1524 Fragments arranged by size: 1554 1524 787 597 531 393 268 176 63 EcoRI G'AATT_C Cuts at: 2173 2484 2681 3202 3580 2173 Size: 311 197 521 378 4486 Fragments arranged by size: 4486 521 378 311 197 EcoRV GAT'ATC Cuts at: 2536 2536 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2383 2415 4534 4566 4608 Size: 32 135 32 811 32 2119 32 42 Cuts at: 4608 4640 1373 Size: 32 2626 Fragments arranged by size: 2626 2119 811 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3483 669 Size: 988 281 1299 246 3079 Fragments arranged by size: 3079 1299 988 281 246 HindIII A'AGCT_T Cuts at: 748 2476 748 Size: 1728 4165 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2059 5007 5191 1305 Size: 754 2948 184 2007 Fragments arranged by size: 2948 2007 754 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2136 2775 10 Size: 54 1425 343 304 639 3128 Fragments arranged by size: 3128 1425 639 343 304 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 2520 2706 3851 4210 4369 518 Size: 648 1348 6 186 1145 359 159 2042 Fragments arranged by size: 2042 1348 1145 648 359 186 159 6 MspA1I CmG'CkG Cuts at: 1606 3309 3986 5134 5379 1606 Size: 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3251 4796 1132 Size: 97 2022 1545 2229 Fragments arranged by size: 2229 2022 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 3245 830 Size: 2415 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacI G_AGCT'C Cuts at: 2045 2045 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SexAI A'CCwGG_T Cuts at: 2577 2890 3106 2577 Size: 313 216 5364 Fragments arranged by size: 5364 313 216 SfcI C'TryA_G Cuts at: 826 1045 2994 3241 4376 5057 5248 826 Size: 219 1949 247 1135 681 191 1471 Fragments arranged by size: 1949 1471 1135 681 247 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2025 2479 3997 4898 5160 820 Size: 197 926 82 454 1518 901 262 1553 Fragments arranged by size: 1553 1518 926 901 454 262 197 82 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 2848 3817 5 Size: 47 2796 969 2081 Fragments arranged by size: 2796 2081 969 47 StuI AGG'CCT Cuts at: 1517 2543 1517 Size: 1026 4867 StyI C'CwwG_G Cuts at: 513 1065 2137 2783 3332 513 Size: 552 1072 646 549 3074 Fragments arranged by size: 3074 1072 646 552 549 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3920 3937 4090 4275 4720 325 Size: 1255 738 1602 17 153 185 445 1498 Fragments arranged by size: 1602 1498 1255 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2583 4139 5851 Size: 274 80 33 51 1443 606 1556 1712 Cuts at: 5851 96 Size: 138 Fragments arranged by size: 1712 1556 1443 606 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 2768 2144 Size: 624 5269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 2505 2628 4022 2505 Size: 123 1394 4376 Fragments arranged by size: 4376 1394 123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI