Restriction map for
pFN21AE0661
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AvrII BanII BbeI BbrPI BclI
BfaI BfrI BlnI BlpI BmyI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmAI BsmBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI HaeII HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 70 GACGCC G[GA]^CG[CT]C
AflIII 405 ACACGT A^C[GA][CT]GT
AvaI 152 CCCGGG C^[CT]CG[GA]G
AviII 80 TGCGCA TGC^GCA
BalI 666 TGGCCA TGG^CCA
BamHI 210 GGATCC G^GATCC
BanI 612 GGCACC G^G[CT][GA]CC
BbsI 97 GTCTTC GAAGAC(2/6)
BbvCI 679 CCTCAGC CCTCAGC(-5/-2)
BcgI 83 GCAAGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 347 GGATAC GTATCC(6/5)
BglI 193 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 35 AGATCT A^GATCT
BpuAI 97 GTCTTC GAAGAC(2/6)
BsaBI 211 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 70 GACGCC G[GA]^CG[CT]C
BseRI 112 GAGGAG GAGGAG(10/8)
BsgI 382 GTGCAG GTGCAG(16/14)
BsiEI 132 CGACCG CG[GA][CT]^CG
BsiYI 135 CCGGCAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 135 CCGGCAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 701 GAATGC GAATGC(1/-1)
BsoBI 152 CCCGGG C^[CT]CG[GA]G
BspMI 351 ACCTGC ACCTGC(4/8)
BsrFI 134 ACCGGC [GA]^CCGG[CT]
BstF5I 319 CATCC GGATG(2/0)
CfoI 81 GCGC GCG^C
Cfr10I 134 ACCGGC [GA]^CCGG[CT]
Cfr13I 200 GGGCC G^G[ACGT]CC
DraII 199 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 141 GACGGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 173 CCATGG C^C[GA][CT]GG
EaeI 666 TGGCCA [CT]^GGCC[GA]
EcoO109I 199 AGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 319 CATCC GGATG(9/13)
FspI 80 TGCGCA TGC^GCA
HgaI 70 GACGC GACGC(5/10)
HhaI 81 GCGC GCG^C
Hin1I 70 GACGCC G[GA]^CG[CT]C
HinP1I 81 GCGC G^CGC
MamI 211 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 666 TGGCCA TGG^CCA
MscI 666 TGGCCA TGG^CCA
NcoI 173 CCATGG C^CATGG
Sau96I 200 GGGCC G^G[ACGT]CC
ScaI 86 AGTACT AGT^ACT
SexAI 288 ACCAGGT A^CC[AT]GGT
SmaI 152 CCCGGG CCC^GGG
Tsp45I 169 GTCAC ^GT[GC]AC
TspRI 456 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 242 CCAACGTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BpmI 542 CTCCAG CTGGAG(16/14)
458 CTGGAG
BsmFI 619 GGGAC GGGAC(10/14)
150 GTCCC
BstYI 35 AGATCT [GA]^GATC[CT]
210 GGATCC
EcoT14I 173 CCATGG C^C[AT][AT]GG
274 CCAAGG
MaeIII 169 GTCAC ^GT[ACGT]AC
562 GTTAC
MboII 38 TCTTC GAAGA(8/7)
98 TCTTC
MflI 35 AGATCT [GA]^GATC[CT]
210 GGATCC
NlaIII 174 CATG CATG^
282 CATG
NlaIV 210 GGATCC GG[ACGT]^[ACGT]CC
612 GGCACC
StyI 173 CCATGG C^C[AT][AT]GG
274 CCAAGG
TaqI 92 TCGA T^CGA
110 TCGA
TthHB8I 92 TCGA T^CGA
110 TCGA
XhoII 35 AGATCT [GA]^GATC[CT]
210 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BcnI BsaJI BsrI BssKI
BstAPI BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII PstI PvuII RsaI Sau3AI
ScrFI SfcI TseI