(Circular) (Six-base) MAPSORT of: pFN21AE0654.seq Check: 813 from: 1 to: 5863 pFN21AE0654 5863 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2652 3206 1937 Size: 715 554 4594 Fragments arranged by size: 4594 715 554 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 3926 5310 5578 1632 Size: 750 1544 1384 268 1917 Fragments arranged by size: 1917 1544 1384 750 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2193 3009 3131 1184 Size: 1009 816 122 3916 Fragments arranged by size: 3916 1009 816 122 AlwNI CAG_nnn'CTG Cuts at: 3606 5178 3606 Size: 1572 4291 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 2730 3172 3398 3550 5659 1493 Size: 1237 442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1237 442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2409 3188 1870 Size: 73 466 779 4545 Fragments arranged by size: 4545 779 466 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 3182 1391 Size: 1791 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2533 3042 928 Size: 971 634 509 3749 Fragments arranged by size: 3749 971 634 509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2119 3755 4965 2119 Size: 1636 1210 3017 Fragments arranged by size: 3017 1636 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2931 3097 4474 Size: 107 122 71 631 605 1259 166 1377 Cuts at: 4474 136 Size: 1525 Fragments arranged by size: 1525 1377 1259 631 605 166 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 3924 5080 1790 Size: 2134 1156 2573 Fragments arranged by size: 2573 2134 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3594 4552 Size: 416 53 83 399 24 1137 1040 958 Cuts at: 4552 442 Size: 1753 Fragments arranged by size: 1753 1137 1040 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4523 1239 Size: 3284 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3078 3988 4853 5151 3078 Size: 910 865 298 3790 Fragments arranged by size: 3790 910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5863 BseYI C'CCAG_C Cuts at: 5066 5562 5066 Size: 496 5367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2808 3039 1163 Size: 573 1072 231 3987 Fragments arranged by size: 3987 1072 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3924 4009 5080 3924 Size: 85 1071 4707 Fragments arranged by size: 4707 1071 85 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3182 3924 4009 5080 1391 Size: 399 1392 742 85 1071 2174 Fragments arranged by size: 2174 1392 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 2164 3752 5482 2164 Size: 1588 1730 2545 Fragments arranged by size: 2545 1730 1588 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 844 Size: 2374 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2567 3750 1394 Size: 429 744 1183 3507 Fragments arranged by size: 3507 1183 744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4507 4694 1700 Size: 110 2697 187 2869 Fragments arranged by size: 2869 2697 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2195 2795 794 Size: 1401 600 3862 Fragments arranged by size: 3862 1401 600 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 2039 1184 Size: 855 5008 BstXI CCAn_nnnn'nTGG Cuts at: 2519 2921 2942 2519 Size: 402 21 5440 Fragments arranged by size: 5440 402 21 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2653 2653 Size: 5863 BtgI C'CryG_G Cuts at: 513 1065 2004 3059 513 Size: 552 939 1055 3317 Fragments arranged by size: 3317 1055 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2509 2923 2962 Size: 54 1425 50 275 18 679 414 39 Cuts at: 2962 3010 3310 4199 5828 8 Size: 48 300 889 1629 43 Fragments arranged by size: 1629 1425 889 679 414 300 275 54 50 48 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 1353 Size: 2440 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 3926 4523 5310 5578 1239 Size: 393 750 1544 597 787 268 1524 Fragments arranged by size: 1544 1524 787 750 597 393 268 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 EcoRV GAT'ATC Cuts at: 2909 2909 Size: 5863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2882 2882 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2050 2082 4504 4536 4578 Size: 32 135 32 478 32 2422 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 2422 478 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 2799 3462 2799 Size: 663 5200 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2511 2925 2964 3012 10 Size: 54 1425 343 679 414 39 48 2861 Fragments arranged by size: 2861 1425 679 414 343 54 48 39 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2322 2784 3821 4180 4339 518 Size: 648 1009 147 462 1037 359 159 2042 Fragments arranged by size: 2042 1037 1009 648 462 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2132 2183 3279 3956 5104 5349 1606 Size: 526 51 1096 677 1148 245 2120 Fragments arranged by size: 2120 1148 1096 677 526 245 51 NcoI C'CATG_G Cuts at: 513 1065 2004 3059 513 Size: 552 939 1055 3317 Fragments arranged by size: 3317 1055 939 552 NdeI CA'TA_TG Cuts at: 387 2581 2608 2998 387 Size: 2194 27 390 3252 Fragments arranged by size: 3252 2194 390 27 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2798 3115 3221 4766 1132 Size: 97 1569 317 106 1545 2229 Fragments arranged by size: 2229 1569 1545 317 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 3077 1108 Size: 1969 3894 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PstI C_TGCA'G Cuts at: 830 2188 3215 830 Size: 1358 1027 3478 Fragments arranged by size: 3478 1358 1027 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2132 2183 2132 Size: 51 5812 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 2184 3211 4346 5027 5218 826 Size: 219 1139 1027 1135 681 191 1471 Fragments arranged by size: 1471 1139 1135 1027 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2931 2931 Size: 5863 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3093 3967 4868 5130 820 Size: 197 926 1150 874 901 262 1553 Fragments arranged by size: 1553 1150 926 901 874 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2004 3059 3302 513 Size: 552 939 1055 243 3074 Fragments arranged by size: 3074 1055 939 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2495 3890 3907 4060 4245 4690 325 Size: 1255 915 1395 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2576 2645 4109 Size: 274 80 33 51 1443 599 69 1464 Cuts at: 4109 5821 96 Size: 1712 138 Fragments arranged by size: 1712 1464 1443 599 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 3046 1316 Size: 1730 4133 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 2500 2519 2330 Size: 170 19 5674 Fragments arranged by size: 5674 170 19 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI