Restriction map for
pFN21AE0653
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcsI 505 AAATTC [GA]^AATT[CT]
AfaI 108 GTAC GT^AC
AlwI 384 GGATC GGATC(4/5)
AlwNI 373 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 505 AAATTC [GA]^AATT[CT]
AspHI 130 GAGCTC G[AT]GC[AT]^C
AvaI 185 CCCGAG C^[CT]CG[GA]G
BalI 231 TGGCCA TGG^CCA
BanII 130 GAGCTC G[GA]GC[CT]^C
BbsI 466 GAAGAC GAAGAC(2/6)
BbvI 365 GCAGC GCAGC(8/12)
BcgI 65 CGACCGACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 322 AGATCT A^GATCT
BpuAI 466 GAAGAC GAAGAC(2/6)
BsaAI 49 TACGTG [CT]AC^GT[GA]
BsaI 581 GGTCTC GGTCTC(1/5)
BsgI 424 GTGCAG GTGCAG(16/14)
BsiHKAI 130 GAGCTC G[AT]GC[AT]^C
BsmAI 582 GTCTC GTCTC(1/5)
BsoBI 185 CCCGAG C^[CT]CG[GA]G
BspHI 516 TCATGA T^CATGA
BsrBI 510 CCGCTC CCGCTC(-3/-3)
BsrFI 140 ACCGGC [GA]^CCGG[CT]
BssKI 260 CCAGG ^CC[ACGT]GG
BssSI 151 CTCGTG CACGAG(-5/-1)
BstAPI 193 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 260 CCAGG CC^[AT]GG
BstYI 322 AGATCT [GA]^GATC[CT]
Cac8I 368 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 140 ACCGGC [GA]^CCGG[CT]
DsaI 291 CCGTGG C^C[GA][CT]GG
EagI 111 CGGCCG C^GGCCG
EclXI 111 CGGCCG C^GGCCG
Eco52I 111 CGGCCG C^GGCCG
EcoNI 355 CCTCCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 260 CCAGG ^CC[AT]GG
HapII 141 CCGG C^CGG
HinfI 607 GACTC G^A[ACGT]TC
HpaII 141 CCGG C^CGG
HphI 523 GGTGA GGTGA(8/7)
MaeIII 605 GTGAC ^GT[ACGT]AC
MflI 322 AGATCT [GA]^GATC[CT]
MluNI 231 TGGCCA TGG^CCA
MscI 231 TGGCCA TGG^CCA
MspA1I 366 CAGCTG C[AC]G^C[GT]G
MspI 141 CCGG C^CGG
MvaI 260 CCAGG CC^[AT]GG
NspI 389 ACATGC [GA]CATG^[CT]
PleI 607 GACTC GAGTC(4/5)
PvuII 366 CAGCTG CAG^CTG
RcaI 516 TCATGA T^CATGA
RsaI 108 GTAC GT^AC
SacI 130 GAGCTC GAGCT^C
ScrFI 260 CCAGG CC^[ACGT]GG
SmlI 133 CTCAAG C^T[CT][GA]AG
TseI 365 GCAGC G^C[AT]GC
Tsp45I 605 GTGAC ^GT[GC]AC
Tsp509I 506 AATT ^AATT
TspRI 235 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 322 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BmyI 130 GAGCTC G[AGT]GC[ACT]^C
154 GTGCCC
BsaJI 259 CCCAGG C^C[ACGT][ACGT]GG
291 CCGTGG
BsiEI 65 CGACCG CG[GA][CT]^CG
111 CGGCCG
Bsp1286I 130 GAGCTC G[AGT]GC[ACT]^C
154 GTGCCC
BsrI 492 ACTGG ACTGG(1/-1)
234 CCAGT
DdeI 457 CTCAG C^T[ACGT]AG
551 CTGAG
EaeI 111 CGGCCG [CT]^GGCC[GA]
231 TGGCCA
MaeII 50 ACGT A^CGT
71 ACGT
MslI 45 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
517 CATGAGGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AluI AvaII BsiYI
BslI BstF5I BstUI CfoI Cfr13I
DpnII DraII EcoO109I Fnu4HI FokI
HaeIII HhaI HinP1I ItaI MboI
MboII MnlI MvnI MwoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
SfaNI TaqI TthHB8I