Restriction map for
pFN21AE0653
(Linear) (Six-base) MAPSORT of: KIEE0653.seq Check: 8370 from: 1 to: 663
KIEE0653.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 663
Size: 400 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 663
Size: 82 32 549
Fragments arranged by size:
549 82 32
AlwNI CAG_nnn'CTG
Cuts at: 0 378 663
Size: 378 285
ApoI r'AATT_y
Cuts at: 0 505 663
Size: 505 158
AvaI C'yCGr_G
Cuts at: 0 185 663
Size: 185 478
BanII G_rGCy'C
Cuts at: 0 134 663
Size: 134 529
BbsI GAAGACnn'nnnn_
Cuts at: 0 473 663
Size: 473 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 88 663
Size: 54 34 575
Fragments arranged by size:
575 54 34
BglII A'GATC_T
Cuts at: 0 322 663
Size: 322 341
Bme1580I G_kGCm'C
Cuts at: 0 158 663
Size: 158 505
BmgBI CAC'GTC
Cuts at: 0 72 663
Size: 72 591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 663
Size: 118 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 587 663
Size: 587 76
BsaAI yAC'GTr
Cuts at: 0 51 663
Size: 51 612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 663
Size: 82 30 551
Fragments arranged by size:
551 82 30
BseYI C'CCAG_C
Cuts at: 0 8 400 663
Size: 8 392 263
Fragments arranged by size:
392 263 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 663
Size: 445 218
BsiEI CG_ry'CG
Cuts at: 0 68 114 663
Size: 68 46 549
Fragments arranged by size:
549 68 46
BsiHKAI G_wGCw'C
Cuts at: 0 134 663
Size: 134 529
Bsp1286I G_dGCh'C
Cuts at: 0 134 158 663
Size: 134 24 505
Fragments arranged by size:
505 134 24
BspHI T'CATG_A
Cuts at: 0 516 663
Size: 516 147
BsrBI CCG'CTC
Cuts at: 0 512 663
Size: 512 151
BsrFI r'CCGG_y
Cuts at: 0 140 663
Size: 140 523
BssSI C'ACGA_G
Cuts at: 0 151 663
Size: 151 512
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 663
Size: 199 464
BstYI r'GATC_y
Cuts at: 0 322 663
Size: 322 341
BtgI C'CryG_G
Cuts at: 0 291 663
Size: 291 372
EaeI y'GGCC_r
Cuts at: 0 111 231 663
Size: 111 120 432
Fragments arranged by size:
432 120 111
EagI C'GGCC_G
Cuts at: 0 111 663
Size: 111 552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 663
Size: 28 635
EcoICRI GAG'CTC
Cuts at: 0 132 663
Size: 132 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 663
Size: 400 263
EcoNI CCTnn'n_nnAGG
Cuts at: 0 359 663
Size: 359 304
EcoO109I rG'GnC_Cy
Cuts at: 0 93 180 375 656 663
Size: 93 87 195 281 7
Fragments arranged by size:
281 195 93 87 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 663
Size: 88 575
MscI TGG'CCA
Cuts at: 0 233 663
Size: 233 430
MslI CAynn'nnrTG
Cuts at: 0 49 521 663
Size: 49 472 142
Fragments arranged by size:
472 142 49
MspA1I CmG'CkG
Cuts at: 0 368 663
Size: 368 295
NspI r_CATG'y
Cuts at: 0 393 663
Size: 393 270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 663
Size: 82 32 549
Fragments arranged by size:
549 82 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 180 375 663
Size: 93 87 195 288
Fragments arranged by size:
288 195 93 87
PvuII CAG'CTG
Cuts at: 0 368 663
Size: 368 295
SacI G_AGCT'C
Cuts at: 0 134 663
Size: 134 529
SmlI C'TyrA_G
Cuts at: 0 133 663
Size: 133 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 272 575 663
Size: 82 190 303 88
Fragments arranged by size:
303 190 88 82
Enzymes that do cut:
AcuI AloI AlwNI ApoI AvaI BanII BbsI BcgI
BglII Bme1580I BmgBI BpuEI BsaI BsaAI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssSI
BstAPI BstYI BtgI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I MmeI MscI MslI MspA1I NspI PpiI
PpuMI PvuII SacI SmlI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BplI BpmI
Bpu10I BsaBI BsaHI BsaWI BseRI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI