(Circular) (Six-base) MAPSORT of: pFN21AE0624.seq Check: 8177 from: 1 to: 5815 pFN21AE0624 5815 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 3878 5262 5530 1632 Size: 954 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 954 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 4045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 2137 2380 3029 3558 5130 2137 Size: 243 649 529 1572 2822 Fragments arranged by size: 2822 1572 649 529 243 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 3124 3350 3502 5611 1493 Size: 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 226 152 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2515 3140 1870 Size: 73 572 625 4545 Fragments arranged by size: 4545 625 572 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2969 3136 4591 618 Size: 325 217 627 1182 167 1455 1842 Fragments arranged by size: 1842 1455 1182 627 325 217 167 BanII G_rGCy'C Cuts at: 1391 2313 3134 1391 Size: 922 821 4072 Fragments arranged by size: 4072 922 821 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 928 Size: 971 573 4271 Fragments arranged by size: 4271 971 573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2654 2688 3048 3082 4006 Size: 34 50 34 633 34 360 34 924 Cuts at: 4006 4040 1903 Size: 34 3678 Fragments arranged by size: 3678 924 633 360 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2546 3707 4917 2546 Size: 1161 1210 3444 Fragments arranged by size: 3444 1210 1161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BfrBI ATG'CAT Cuts at: 2154 2154 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 4426 136 Size: 107 122 71 631 605 493 2261 1525 Fragments arranged by size: 2261 1525 631 605 493 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2087 2972 3083 3876 5032 1790 Size: 297 885 111 793 1156 2573 Fragments arranged by size: 2573 1156 885 793 297 111 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2737 3546 4504 Size: 416 53 83 399 24 1320 809 958 Cuts at: 4504 442 Size: 1753 Fragments arranged by size: 1753 1320 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 4475 1239 Size: 953 2283 2579 Fragments arranged by size: 2579 2283 953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3940 4805 5103 2414 Size: 1526 865 298 3126 Fragments arranged by size: 3126 1526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2557 3606 493 Size: 711 1353 1049 2702 Fragments arranged by size: 2702 1353 1049 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2355 3622 4004 4639 Size: 53 83 186 936 822 1267 382 635 Cuts at: 4639 275 Size: 1451 Fragments arranged by size: 1451 1267 936 822 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5815 BseYI C'CCAG_C Cuts at: 2008 2965 3029 5018 5514 2008 Size: 957 64 1989 496 2309 Fragments arranged by size: 2309 1989 957 496 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 4780 Fragments arranged by size: 4780 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 2529 3876 3961 5032 2313 Size: 216 1347 85 1071 3096 Fragments arranged by size: 3096 1347 1071 216 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2313 2529 2972 3083 3134 3876 Size: 399 297 226 216 443 111 51 742 Cuts at: 3876 3961 5032 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 297 226 216 111 85 51 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 2478 3704 5434 2478 Size: 1226 1730 2859 Fragments arranged by size: 2859 1730 1226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 3170 844 Size: 1562 764 3489 Fragments arranged by size: 3489 1562 764 BsrBI CCG'CTC Cuts at: 1394 1823 2350 3702 1394 Size: 429 527 1352 3507 Fragments arranged by size: 3507 1352 527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 4310 4484 65 Size: 1183 906 2156 174 1396 Fragments arranged by size: 2156 1396 1183 906 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3037 4459 4646 1700 Size: 110 1227 1422 187 2869 Fragments arranged by size: 2869 1422 1227 187 110 BsrGI T'GTAC_A Cuts at: 96 2416 96 Size: 2320 3495 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 794 Size: 2021 3794 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2645 1184 Size: 1461 4354 BstXI CCAn_nnnn'nTGG Cuts at: 2938 2938 Size: 5815 BstYI r'GATC_y Cuts at: 1972 2425 3145 3512 3897 3914 5366 5452 5464 Size: 453 720 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 720 453 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2825 2931 3004 513 Size: 552 1760 106 73 3324 Fragments arranged by size: 3324 1760 552 106 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2608 487 Size: 683 56 1382 3694 Fragments arranged by size: 3694 1382 683 56 BtsI GCAGTG_nn' Cuts at: 2022 2527 3098 3331 4125 4145 2022 Size: 505 571 233 794 20 3692 Fragments arranged by size: 3692 794 571 505 233 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3262 4151 Size: 54 1425 50 275 18 312 1120 889 Cuts at: 4151 5780 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1120 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 4402 4776 4922 387 Size: 1064 138 468 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 468 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2311 Size: 5815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2586 3878 4475 5262 5530 1239 Size: 393 560 394 1292 597 787 268 1524 Fragments arranged by size: 1524 1292 787 597 560 394 393 268 EcoO109I rG'GnC_Cy Cuts at: 2024 2091 2167 2512 2782 2024 Size: 67 76 345 270 5057 Fragments arranged by size: 5057 345 270 76 67 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2911 2938 2911 Size: 27 5788 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 4962 1678 Size: 3284 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2594 2626 2723 Size: 32 135 32 881 32 109 32 97 Cuts at: 2723 2755 4456 4488 4530 4562 1373 Size: 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 881 135 109 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 4929 5113 1305 Size: 1206 2418 184 2007 Fragments arranged by size: 2418 2007 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 3681 Fragments arranged by size: 3681 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 3773 4132 4291 518 Size: 648 1770 837 359 159 2042 Fragments arranged by size: 2042 1770 837 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2293 2965 3044 3231 3908 5056 5301 1606 Size: 687 672 79 187 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 672 245 187 79 NaeI GCC'GGC Cuts at: 3039 3039 Size: 5815 NcoI C'CATG_G Cuts at: 513 1065 2825 3004 513 Size: 552 1760 179 3324 Fragments arranged by size: 3324 1760 552 179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NgoMIV G'CCGG_C Cuts at: 3037 3037 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NsiI A_TGCA'T Cuts at: 2156 2156 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3173 4718 1132 Size: 97 1944 1545 2229 Fragments arranged by size: 2229 1944 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 3029 1108 Size: 1272 649 3894 Fragments arranged by size: 3894 1272 649 PfoI T'CCnGG_A Cuts at: 2548 2548 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2782 2512 Size: 270 5545 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PstI C_TGCA'G Cuts at: 830 2964 3167 830 Size: 2134 203 3478 Fragments arranged by size: 3478 2134 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2293 2965 3044 2293 Size: 672 79 5064 Fragments arranged by size: 5064 672 79 SacI G_AGCT'C Cuts at: 2313 2313 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2452 2587 2960 3163 4298 4979 5170 Size: 219 1407 135 373 203 1135 681 191 Cuts at: 5170 826 Size: 1471 Fragments arranged by size: 1471 1407 1135 681 373 219 203 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 2517 3142 1872 Size: 645 625 4545 Fragments arranged by size: 4545 645 625 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3919 4820 5082 820 Size: 197 926 486 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 486 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 2813 1517 Size: 1296 4519 StyI C'CwwG_G Cuts at: 513 1065 2160 2825 3004 3254 513 Size: 552 1095 665 179 250 3074 Fragments arranged by size: 3074 1095 665 552 250 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2838 3842 3859 4012 4197 4642 325 Size: 1255 1258 1004 17 153 185 445 1498 Fragments arranged by size: 1498 1258 1255 1004 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2416 2448 4061 Size: 274 80 33 51 1443 439 32 1613 Cuts at: 4061 5773 96 Size: 1712 138 Fragments arranged by size: 1712 1613 1443 439 274 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 2848 3151 2848 Size: 303 5512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2167 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 2515 3140 1870 Size: 645 625 4545 Fragments arranged by size: 4545 645 625 XmnI GAAnn'nnTTC Cuts at: 2795 3944 2795 Size: 1149 4666 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI