(Circular) (Six-base) MAPSORT of: pFN21AE0620.seq Check: 951 from: 1 to: 5785 pFN21AE0620 5785 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3848 5232 5500 1632 Size: 2216 1384 268 1917 Fragments arranged by size: 2216 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 2200 4514 2200 Size: 2314 3471 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2020 2644 3528 5100 2020 Size: 624 884 1572 2705 Fragments arranged by size: 2705 1572 884 624 ApaI G_GGCC'C Cuts at: 2054 2054 Size: 5785 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 3094 3320 3472 5581 1493 Size: 1601 226 152 2109 1697 Fragments arranged by size: 2109 1697 1601 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2499 3110 1870 Size: 73 556 611 4545 Fragments arranged by size: 4545 611 556 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2825 3106 4561 618 Size: 325 217 627 1038 281 1455 1842 Fragments arranged by size: 1842 1455 1038 627 325 281 217 BanII G_rGCy'C Cuts at: 1391 2054 2400 2595 3104 1391 Size: 663 346 195 509 4072 Fragments arranged by size: 4072 663 509 346 195 BbeI G_GCGC'C Cuts at: 2829 2829 Size: 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 2636 928 Size: 971 434 303 4077 Fragments arranged by size: 4077 971 434 303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2944 2978 3976 4010 1903 Size: 34 1007 34 998 34 3678 Fragments arranged by size: 3678 1007 998 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 2063 2077 Size: 107 122 71 631 605 386 5 14 Cuts at: 2077 2546 2796 3021 4396 136 Size: 469 250 225 1375 1525 Fragments arranged by size: 1525 1375 631 605 469 386 250 225 122 107 71 14 5 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 2589 3202 2589 Size: 613 5172 Bme1580I G_kGCm'C Cuts at: 1790 2054 3846 5002 1790 Size: 264 1792 1156 2573 Fragments arranged by size: 2573 1792 1156 264 BmgBI CAC'GTC Cuts at: 1318 2463 1318 Size: 1145 4640 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 2349 2437 Size: 416 53 83 399 24 613 319 88 Cuts at: 2437 3516 4474 442 Size: 1079 958 1753 Fragments arranged by size: 1753 1079 958 613 416 399 319 88 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4445 1239 Size: 3206 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3058 4448 882 Size: 617 1559 1390 2219 Fragments arranged by size: 2219 1559 1390 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 2771 5779 2771 Size: 3008 2777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2748 2826 3592 3974 Size: 53 83 186 936 1215 78 766 382 Cuts at: 3974 4609 275 Size: 635 1451 Fragments arranged by size: 1451 1215 936 766 635 382 186 83 78 53 BsaWI w'CCGG_w Cuts at: 2860 4218 4616 4890 5037 5448 2860 Size: 1358 398 274 147 411 3197 Fragments arranged by size: 3197 1358 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5785 BseYI C'CCAG_C Cuts at: 2578 2706 4988 5484 2578 Size: 128 2282 496 2879 Fragments arranged by size: 2879 2282 496 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2452 2769 3235 3996 5024 Size: 275 186 57 394 317 466 761 1028 Cuts at: 5024 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 466 394 317 275 186 57 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2400 2595 3104 3846 3931 5002 Size: 399 264 346 195 509 742 85 1071 Cuts at: 5002 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 509 399 346 264 195 85 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 3040 3140 844 Size: 1304 892 100 3489 Fragments arranged by size: 3489 1304 892 100 BsrBI CCG'CTC Cuts at: 1394 1823 2787 3672 1394 Size: 429 964 885 3507 Fragments arranged by size: 3507 964 885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 2828 4429 4616 1700 Size: 110 247 771 1601 187 2869 Fragments arranged by size: 2869 1601 771 247 187 110 BsrGI T'GTAC_A Cuts at: 96 2847 96 Size: 2751 3034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2644 794 Size: 1850 3935 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstYI r'GATC_y Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780 Size: 1143 367 385 17 1452 86 12 346 Cuts at: 5780 1972 Size: 1977 Fragments arranged by size: 1977 1452 1143 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 3000 513 Size: 552 939 24 972 3298 Fragments arranged by size: 3298 972 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2992 487 Size: 683 56 1766 3280 Fragments arranged by size: 3280 1766 683 56 BtsI GCAGTG_nn' Cuts at: 3301 4095 4115 3301 Size: 794 20 4971 Fragments arranged by size: 4971 794 20 ClaI AT'CG_AT Cuts at: 1554 2775 1554 Size: 1221 4564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2449 2997 Size: 54 1425 50 275 18 225 394 548 Cuts at: 2997 3003 3232 4121 5750 8 Size: 6 229 889 1629 43 Fragments arranged by size: 1629 1425 889 548 394 275 229 225 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2055 2449 3232 1537 Size: 275 243 394 783 4090 Fragments arranged by size: 4090 783 394 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 2441 2538 3715 1353 Size: 830 258 97 1177 3423 Fragments arranged by size: 3423 1177 830 258 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2659 4372 4746 4892 387 Size: 1064 138 1070 1713 374 146 1280 Fragments arranged by size: 1713 1280 1070 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3848 4445 5232 5500 1239 Size: 393 2216 597 787 268 1524 Fragments arranged by size: 2216 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2050 2687 2050 Size: 637 5148 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 5785 FseI GG_CCGG'CC Cuts at: 2061 2061 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2645 2680 2829 4932 1678 Size: 967 35 149 2103 2531 Fragments arranged by size: 2531 2103 967 149 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 4426 4458 4500 Size: 32 135 32 614 32 2208 32 42 Cuts at: 4500 4532 1373 Size: 32 2626 Fragments arranged by size: 2626 2208 614 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3129 3375 669 Size: 988 281 196 995 246 3079 Fragments arranged by size: 3079 995 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KasI G'GCGC_C Cuts at: 2825 2825 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3005 10 Size: 54 1425 343 1173 2790 Fragments arranged by size: 2790 1425 1173 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2474 2843 3743 4102 4261 518 Size: 648 1170 138 369 900 359 159 2042 Fragments arranged by size: 2042 1170 900 648 369 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2394 2578 2588 2890 3201 3878 5026 5271 Size: 788 184 10 302 311 677 1148 245 Cuts at: 5271 1606 Size: 2120 Fragments arranged by size: 2120 1148 788 677 311 302 245 184 10 NaeI GCC'GGC Cuts at: 2059 2830 2059 Size: 771 5014 NarI GG'CG_CC Cuts at: 2826 2826 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 2028 3000 513 Size: 552 939 24 972 3298 Fragments arranged by size: 3298 972 939 552 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NgoMIV G'CCGG_C Cuts at: 2057 2828 2057 Size: 771 5014 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NruI TCG'CGA Cuts at: 2612 2612 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2087 1108 Size: 979 4806 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2050 2050 Size: 5785 PstI C_TGCA'G Cuts at: 830 3051 3137 830 Size: 2221 86 3478 Fragments arranged by size: 3478 2221 86 PvuI CG_AT'CG Cuts at: 2001 2769 2001 Size: 768 5017 PvuII CAG'CTG Cuts at: 2588 2890 2588 Size: 302 5483 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SapI GCTCTTCn'nnn_ Cuts at: 2441 2441 Size: 5785 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2481 2481 Size: 5785 SfcI C'TryA_G Cuts at: 826 1045 3047 3133 4268 4949 5140 826 Size: 219 2002 86 1135 681 191 1471 Fragments arranged by size: 2002 1471 1135 681 219 191 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2058 2063 2058 Size: 5 5780 SfoI GGC'GCC Cuts at: 2827 2827 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2086 2242 3000 3224 513 Size: 552 939 24 58 156 758 224 3074 Fragments arranged by size: 3074 939 758 552 224 156 58 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 3812 3829 3982 4167 4612 325 Size: 1255 1086 1146 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1146 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2847 4031 5743 Size: 274 80 33 51 1443 870 1184 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1712 1443 1184 870 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 2483 1316 Size: 1167 4618 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SrfI SwaI XcmI