(Circular) (Six-base) MAPSORT of: pFN21AE0589.seq Check: 8512 from: 1 to: 6091 pFN21AE0589 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4154 5538 5806 1632 Size: 2522 1384 268 1917 Fragments arranged by size: 2522 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2898 3144 4990 5967 1203 Size: 1695 246 1846 977 1327 Fragments arranged by size: 1846 1695 1327 977 246 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 2257 3834 5406 2257 Size: 1577 1572 2942 Fragments arranged by size: 2942 1577 1572 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AscI GG'CGCG_CC Cuts at: 2549 2549 Size: 6091 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3054 3120 3416 1870 Size: 73 1111 66 296 4545 Fragments arranged by size: 4545 1111 296 73 66 BamHI G'GATC_C Cuts at: 2266 3421 3788 2266 Size: 1155 367 4569 Fragments arranged by size: 4569 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 3115 3412 4867 618 Size: 325 217 627 893 435 297 1455 1842 Fragments arranged by size: 1842 1455 893 627 435 325 297 217 BanII G_rGCy'C Cuts at: 1391 2953 3410 1391 Size: 1562 457 4072 Fragments arranged by size: 4072 1562 457 BbeI G_GCGC'C Cuts at: 3119 3119 Size: 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 3118 928 Size: 971 213 1006 3901 Fragments arranged by size: 3901 1006 971 213 BbvCI CC'TCA_GC Cuts at: 2171 2171 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2411 3508 2411 Size: 1097 4994 Bme1580I G_kGCm'C Cuts at: 1790 2685 4152 5308 1790 Size: 895 1467 1156 2573 Fragments arranged by size: 2573 1467 1156 895 BmgBI CAC'GTC Cuts at: 1318 2401 1318 Size: 1083 5008 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2215 2735 3193 4751 1239 Size: 976 520 458 1558 2579 Fragments arranged by size: 2579 1558 976 520 458 Bpu10I CC'TnA_GC Cuts at: 2171 2171 Size: 6091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 4216 5081 5379 2927 Size: 1289 865 298 3639 Fragments arranged by size: 3639 1289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2181 4754 882 Size: 617 682 2573 2219 Fragments arranged by size: 2573 2219 682 617 BsaAI yAC'GTr Cuts at: 493 1204 3151 3882 493 Size: 711 1947 731 2702 Fragments arranged by size: 2702 1947 731 711 BsaBI GATnn'nnATC Cuts at: 2750 6085 2750 Size: 3335 2756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 3116 3898 4280 Size: 53 83 186 936 939 644 782 382 Cuts at: 4280 4915 275 Size: 635 1451 Fragments arranged by size: 1451 939 936 782 644 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3043 1186 Size: 1857 4234 BseYI C'CCAG_C Cuts at: 2199 2503 5294 5790 2199 Size: 304 2791 496 2500 Fragments arranged by size: 2791 2500 496 304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 3295 1163 Size: 573 1044 515 3959 Fragments arranged by size: 3959 1044 573 515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3071 4152 4237 5308 3071 Size: 1081 85 1071 3854 Fragments arranged by size: 3854 1081 1071 85 BsmI GAATG_Cn' Cuts at: 2143 3602 3695 2143 Size: 1459 93 4539 Fragments arranged by size: 4539 1459 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2685 2953 3071 3410 4152 4237 5308 Size: 399 895 268 118 339 742 85 1071 Cuts at: 5308 1391 Size: 2174 Fragments arranged by size: 2174 1071 895 742 399 339 268 118 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3312 3446 844 Size: 2468 134 3489 Fragments arranged by size: 3489 2468 134 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2838 4586 4760 65 Size: 1183 1590 1748 174 1396 Fragments arranged by size: 1748 1590 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2870 3018 4735 4922 1700 Size: 110 1060 148 1717 187 2869 Fragments arranged by size: 2869 1717 1060 187 148 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 2549 1925 Size: 624 5467 BssSI C'ACGA_G Cuts at: 2373 4151 5163 2373 Size: 1778 1012 3301 Fragments arranged by size: 3301 1778 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2995 1184 Size: 1811 4280 BstXI CCAn_nnnn'nTGG Cuts at: 2713 2929 3082 3208 2713 Size: 216 153 126 5596 Fragments arranged by size: 5596 216 153 126 BstYI r'GATC_y Cuts at: 1972 2037 2266 3421 3788 4173 4190 5642 5728 Size: 65 229 1155 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1155 385 367 346 229 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 513 Size: 552 960 4579 Fragments arranged by size: 4579 960 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2334 487 Size: 683 56 1108 4244 Fragments arranged by size: 4244 1108 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2544 2670 2775 3607 4401 4421 2070 Size: 474 126 105 832 794 20 3740 Fragments arranged by size: 3740 832 794 474 126 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3176 5098 809 Size: 507 1860 1922 1802 Fragments arranged by size: 1922 1860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2365 2435 2703 Size: 54 1425 50 275 18 535 70 268 Cuts at: 2703 3006 3198 3210 3538 4427 6056 8 Size: 303 192 12 328 889 1629 43 Fragments arranged by size: 1629 1425 889 535 328 303 275 268 192 70 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4021 1353 Size: 2668 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2231 4678 5052 5198 387 Size: 1064 138 642 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 642 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2735 3193 4154 4751 5538 5806 Size: 393 583 520 458 961 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 961 787 597 583 520 458 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2654 2654 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 2047 2203 2342 2454 2574 2938 2047 Size: 156 139 112 120 364 5200 Fragments arranged by size: 5200 364 156 139 120 112 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 EcoRV GAT'ATC Cuts at: 2614 2614 Size: 6091 FspI TGC'GCA Cuts at: 2699 4597 2699 Size: 1898 4193 FspAI rTGC'GCAy Cuts at: 2699 2699 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2600 3119 5238 1678 Size: 922 519 2119 2531 Fragments arranged by size: 2531 2119 922 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2900 2932 3273 3305 3346 Size: 32 135 32 1328 32 341 32 41 Cuts at: 3346 3378 4732 4764 4806 4838 1373 Size: 32 1354 32 42 32 2626 Fragments arranged by size: 2626 1354 1328 341 135 42 41 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2767 2868 3435 3681 669 Size: 988 281 829 101 567 246 3079 Fragments arranged by size: 3079 988 829 567 281 246 101 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 3115 3115 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3304 5205 5389 1305 Size: 1999 1901 184 2007 Fragments arranged by size: 2007 1999 1901 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2367 2437 3200 3212 10 Size: 54 1425 343 535 70 763 12 2889 Fragments arranged by size: 2889 1425 763 535 343 70 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2624 3149 4049 4408 4567 518 Size: 648 1458 525 900 359 159 2042 Fragments arranged by size: 2042 1458 900 648 525 359 159 MspA1I CmG'CkG Cuts at: 1606 2315 2363 2406 3507 4184 5332 5577 1606 Size: 709 48 43 1101 677 1148 245 2120 Fragments arranged by size: 2120 1148 1101 709 677 245 48 43 NarI GG'CG_CC Cuts at: 3116 3116 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2025 513 Size: 552 960 4579 Fragments arranged by size: 4579 960 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2902 3148 3449 4994 1132 Size: 97 1673 246 301 1545 2229 Fragments arranged by size: 2229 1673 1545 301 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 2898 3144 4990 2898 Size: 246 1846 3999 Fragments arranged by size: 3999 1846 246 PflMI CCAn_nnn'nTGG Cuts at: 1108 2692 1108 Size: 1584 4507 PfoI T'CCnGG_A Cuts at: 2712 3364 2712 Size: 652 5439 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2203 2342 2454 2938 2047 Size: 156 139 112 484 5200 Fragments arranged by size: 5200 484 156 139 112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 2064 2658 3051 3443 830 Size: 1234 594 393 392 3478 Fragments arranged by size: 3478 1234 594 393 392 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2315 2363 2315 Size: 48 6043 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SanDI GG'GwC_CC Cuts at: 2047 2342 2047 Size: 295 5796 SbfI CC_TGCA'GG Cuts at: 2064 2658 3443 2064 Size: 594 785 4712 Fragments arranged by size: 4712 785 594 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2060 2491 2654 3047 3439 4574 5255 Size: 219 1015 431 163 393 392 1135 681 Cuts at: 5255 5446 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1015 681 431 393 392 219 191 163 SfoI GGC'GCC Cuts at: 3117 3117 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3056 3122 3418 1872 Size: 1184 66 296 4545 Fragments arranged by size: 4545 1184 296 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 4195 5096 5358 820 Size: 197 926 999 1253 901 262 1553 Fragments arranged by size: 1553 1253 999 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2025 2223 2296 3189 3530 513 Size: 552 960 198 73 893 341 3074 Fragments arranged by size: 3074 960 893 552 341 198 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2935 4118 4135 4288 4473 4918 325 Size: 1255 1355 1183 17 153 185 445 1498 Fragments arranged by size: 1498 1355 1255 1183 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 2936 1316 Size: 1620 4471 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3054 3120 3416 1870 Size: 1184 66 296 4545 Fragments arranged by size: 4545 1184 296 66 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI