(Circular) (Six-base) MAPSORT of: pFN21AE0581.seq Check: 8016 from: 1 to: 5554 pFN21AE0581 5554 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3617 5001 5269 1632 Size: 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 268 AfeI AGC'GCT Cuts at: 2052 2052 Size: 5554 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 2435 1166 Size: 1269 4285 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2447 2479 2447 Size: 32 5522 AlwNI CAG_nnn'CTG Cuts at: 2629 2827 3297 4869 2629 Size: 198 470 1572 3314 Fragments arranged by size: 3314 1572 470 198 ApaI G_GGCC'C Cuts at: 2444 2444 Size: 5554 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2879 1870 Size: 73 936 4545 Fragments arranged by size: 4545 936 73 BamHI G'GATC_C Cuts at: 2274 2884 3251 2274 Size: 610 367 4577 Fragments arranged by size: 4577 610 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2875 4330 618 Size: 325 217 627 1088 1455 1842 Fragments arranged by size: 1842 1455 1088 627 325 217 BanII G_rGCy'C Cuts at: 1391 2343 2444 2706 2748 2873 1391 Size: 952 101 262 42 125 4072 Fragments arranged by size: 4072 952 262 125 101 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 928 Size: 971 196 4387 Fragments arranged by size: 4387 971 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3745 3779 1903 Size: 34 50 34 1724 34 3678 Fragments arranged by size: 3678 1724 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 2613 1548 Size: 1065 4489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 136 Size: 107 122 71 631 605 2493 1525 Fragments arranged by size: 2493 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2648 2971 2648 Size: 323 5231 Bme1580I G_kGCm'C Cuts at: 1790 2444 2517 3615 4771 1790 Size: 654 73 1098 1156 2573 Fragments arranged by size: 2573 1156 1098 654 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 4243 442 Size: 416 53 83 399 24 1868 958 1753 Fragments arranged by size: 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2161 2336 4214 1239 Size: 821 101 175 1878 2579 Fragments arranged by size: 2579 1878 821 175 101 Bpu10I CC'TnA_GC Cuts at: 2732 2732 Size: 5554 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3679 4544 4842 3679 Size: 865 298 4391 Fragments arranged by size: 4391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2417 4217 882 Size: 617 918 1800 2219 Fragments arranged by size: 2219 1800 918 617 BsaAI yAC'GTr Cuts at: 493 1204 3345 493 Size: 711 2141 2702 Fragments arranged by size: 2702 2141 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3361 3743 4378 275 Size: 53 83 186 936 1828 382 635 1451 Fragments arranged by size: 1828 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2196 3987 4385 4659 4806 5217 2196 Size: 1791 398 274 147 411 2533 Fragments arranged by size: 2533 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5329 Fragments arranged by size: 5329 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5554 BseYI C'CCAG_C Cuts at: 2334 2495 2756 4757 5253 2334 Size: 161 261 2001 496 2635 Fragments arranged by size: 2635 2001 496 261 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 2413 2615 1163 Size: 573 569 108 202 4102 Fragments arranged by size: 4102 573 569 202 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2343 3615 3700 4771 2190 Size: 153 1272 85 1071 2973 Fragments arranged by size: 2973 1272 1071 153 85 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2343 2444 2517 2706 2748 2873 Size: 399 400 153 101 73 189 42 125 Cuts at: 2873 3615 3700 4771 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 189 153 125 101 85 73 42 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 2909 844 Size: 1591 474 3489 Fragments arranged by size: 3489 1591 474 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2670 4198 4385 1700 Size: 110 860 1528 187 2869 Fragments arranged by size: 2869 1528 860 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 2686 1925 Size: 761 4793 BssSI C'ACGA_G Cuts at: 3614 4626 3614 Size: 1012 4542 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 794 Size: 1835 3719 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2764 2581 Size: 183 5371 BstYI r'GATC_y Cuts at: 1972 2274 2364 2884 3251 3636 3653 5105 5191 Size: 302 90 520 367 385 17 1452 86 Cuts at: 5191 5203 5549 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 520 385 367 346 302 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 513 Size: 552 999 4003 Fragments arranged by size: 4003 999 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2692 487 Size: 683 56 1466 3349 Fragments arranged by size: 3349 1466 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 2166 1554 Size: 612 4942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 2586 3001 Size: 54 1425 50 275 18 705 51 415 Cuts at: 3001 3890 5519 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 705 415 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3484 1353 Size: 2131 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 4515 4661 387 Size: 1064 138 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2341 2341 Size: 5554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2161 2336 3617 4214 5001 5269 Size: 393 428 101 175 1281 597 787 268 Cuts at: 5269 1239 Size: 1524 Fragments arranged by size: 1524 1281 787 597 428 393 268 175 101 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 5554 EcoO109I rG'GnC_Cy Cuts at: 2441 2794 2441 Size: 353 5201 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 2054 2763 4701 1678 Size: 376 709 1938 2531 Fragments arranged by size: 2531 1938 709 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 4195 4227 4269 Size: 32 135 32 587 32 2004 32 42 Cuts at: 4269 4301 1373 Size: 32 2626 Fragments arranged by size: 2626 2004 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 2588 10 Size: 54 1425 343 705 51 2976 Fragments arranged by size: 2976 1425 705 343 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2435 2474 2657 3512 3871 4030 518 Size: 648 1269 39 183 855 359 159 2042 Fragments arranged by size: 2042 1269 855 648 359 183 159 39 MspA1I CmG'CkG Cuts at: 1606 2334 2827 2970 3647 4795 5040 1606 Size: 728 493 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 728 677 493 245 143 NcoI C'CATG_G Cuts at: 513 1065 2064 513 Size: 552 999 4003 Fragments arranged by size: 4003 999 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2329 2644 2912 4457 1132 Size: 97 1100 315 268 1545 2229 Fragments arranged by size: 2229 1545 1100 315 268 97 PasI CC'CwG_GG Cuts at: 1348 2677 1348 Size: 1329 4225 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2106 2352 2106 Size: 246 5308 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 2794 Size: 5554 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PspOMI G'GGCC_C Cuts at: 2440 2440 Size: 5554 PstI C_TGCA'G Cuts at: 830 2625 2906 830 Size: 1795 281 3478 Fragments arranged by size: 3478 1795 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2334 2334 Size: 5554 SacI G_AGCT'C Cuts at: 2343 2343 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2621 2902 4037 4718 4909 826 Size: 219 1576 281 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 681 281 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2881 1872 Size: 1009 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3658 4559 4821 820 Size: 197 926 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2644 2912 1132 Size: 1512 268 3774 Fragments arranged by size: 3774 1512 268 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5554 StyI C'CwwG_G Cuts at: 513 1065 2064 2993 513 Size: 552 999 929 3074 Fragments arranged by size: 3074 999 929 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3598 3751 3936 4381 325 Size: 1255 2001 17 153 185 445 1498 Fragments arranged by size: 2001 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3800 5512 96 Size: 274 80 33 51 1443 1823 1712 138 Fragments arranged by size: 1823 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2193 2225 5514 5546 2193 Size: 32 3289 32 2201 Fragments arranged by size: 3289 2201 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2150 1316 Size: 834 4720 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2581 2674 2581 Size: 93 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2879 1870 Size: 1009 4545 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI