(Circular) (Six-base) MAPSORT of: pFN21AE0578.seq Check: 6641 from: 1 to: 5326 pFN21AE0578 5326 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3136 278 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 1937 Size: 732 4594 Acc65I G'GTAC_C Cuts at: 2647 2647 Size: 5326 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 3389 4773 5041 1632 Size: 560 1197 1384 268 1917 Fragments arranged by size: 1917 1384 1197 560 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 2393 4225 5202 1203 Size: 1190 1832 977 1327 Fragments arranged by size: 1832 1327 1190 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 4055 4055 Size: 5326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5326 AlwNI CAG_nnn'CTG Cuts at: 3069 4641 3069 Size: 1572 3754 ApaLI G'TGCA_C Cuts at: 2246 3383 4539 2246 Size: 1137 1156 3033 Fragments arranged by size: 3033 1156 1137 ApoI r'AATT_y Cuts at: 1493 2549 2635 2861 3013 5122 1493 Size: 1056 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 3880 160 Size: 872 2848 1606 Fragments arranged by size: 2848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2517 2651 1870 Size: 73 574 134 4545 Fragments arranged by size: 4545 574 134 73 AvrII C'CTAG_G Cuts at: 2347 2347 Size: 5326 BamHI G'GATC_C Cuts at: 2178 2656 3023 2178 Size: 478 367 4481 Fragments arranged by size: 4481 478 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2187 2647 4102 618 Size: 325 217 627 400 460 1455 1842 Fragments arranged by size: 1842 1455 627 460 400 325 217 BanII G_rGCy'C Cuts at: 1391 2645 1391 Size: 1254 4072 BbeI G_GCGC'C Cuts at: 2191 2191 Size: 5326 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 928 Size: 971 118 4237 Fragments arranged by size: 4237 971 118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 1903 Size: 34 1580 34 3678 Fragments arranged by size: 3678 1580 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2519 3218 4428 2519 Size: 699 1210 3417 Fragments arranged by size: 3417 1210 699 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 4561 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2211 3937 136 Size: 107 122 71 631 605 539 1726 1525 Fragments arranged by size: 1726 1525 631 605 539 122 107 71 BglII A'GATC_T Cuts at: 2226 5321 2226 Size: 3095 2231 BlpI GC'TnA_GC Cuts at: 2743 2743 Size: 5326 Bme1580I G_kGCm'C Cuts at: 1790 2082 2250 3387 4543 1790 Size: 292 168 1137 1156 2573 Fragments arranged by size: 2573 1156 1137 292 168 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2050 3057 4015 Size: 416 53 83 399 24 633 1007 958 Cuts at: 4015 442 Size: 1753 Fragments arranged by size: 1753 1007 958 633 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2387 2387 Size: 5326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2306 3986 1239 Size: 1004 63 1680 2579 Fragments arranged by size: 2579 1680 1004 63 Bpu10I CC'TnA_GC Cuts at: 2083 2083 Size: 5326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2601 3989 882 Size: 617 1102 1388 2219 Fragments arranged by size: 2219 1388 1102 617 BsaAI yAC'GTr Cuts at: 493 1204 2396 2491 3117 493 Size: 711 1192 95 626 2702 Fragments arranged by size: 2702 1192 711 626 95 BsaBI GATnn'nnATC Cuts at: 2318 5320 2318 Size: 3002 2324 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2188 3133 3515 Size: 53 83 186 936 489 166 945 382 Cuts at: 3515 4150 275 Size: 635 1451 Fragments arranged by size: 1451 945 936 635 489 382 186 166 83 53 BsaWI w'CCGG_w Cuts at: 3759 4157 4431 4578 4989 3759 Size: 398 274 147 411 4096 Fragments arranged by size: 4096 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 1178 Size: 30 165 30 639 30 4432 Fragments arranged by size: 4432 639 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 2379 1186 Size: 864 329 4133 Fragments arranged by size: 4133 864 329 BseYI C'CCAG_C Cuts at: 4529 5025 4529 Size: 496 4830 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4753 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2776 3537 4565 1540 Size: 275 186 775 761 1028 2301 Fragments arranged by size: 2301 1028 775 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 3387 3472 4543 2250 Size: 1137 85 1071 3033 Fragments arranged by size: 3033 1137 1071 85 BsmI GAATG_Cn' Cuts at: 2837 2930 2837 Size: 93 5233 BsmBI CGTCTCn'nnnn_ Cuts at: 2070 2070 Size: 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2250 2645 3387 3472 4543 1391 Size: 399 292 168 395 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 395 292 168 85 BspEI T'CCGG_A Cuts at: 4989 4989 Size: 5326 BspHI T'CATG_A Cuts at: 3215 4945 3215 Size: 1730 3596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2681 844 Size: 1837 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3213 1394 Size: 429 1390 3507 Fragments arranged by size: 3507 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3821 3995 65 Size: 1183 2573 174 1396 Fragments arranged by size: 2573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 3970 4157 1700 Size: 110 400 1760 187 2869 Fragments arranged by size: 2869 1760 400 187 110 BsrGI T'GTAC_A Cuts at: 96 2457 96 Size: 2361 2965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 3386 4398 3386 Size: 1012 4314 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5326 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2044 2178 2226 2578 2656 3023 3408 3425 Size: 72 134 48 352 78 367 385 17 Cuts at: 3425 4877 4963 4975 5321 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 352 346 134 86 78 72 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3835 Fragments arranged by size: 3835 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3636 3656 2842 Size: 794 20 4512 Fragments arranged by size: 4512 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2630 2630 Size: 5326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2773 3662 5291 Size: 54 1425 50 275 18 943 889 1629 Cuts at: 5291 8 Size: 43 Fragments arranged by size: 1629 1425 943 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2773 1537 Size: 275 961 4090 Fragments arranged by size: 4090 961 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 1353 Size: 1903 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3913 4287 4433 387 Size: 1064 138 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2243 2306 3389 3986 4773 5041 Size: 393 560 51 63 1083 597 787 268 Cuts at: 5041 1239 Size: 1524 Fragments arranged by size: 1524 1083 787 597 560 393 268 63 51 EcoO109I rG'GnC_Cy Cuts at: 2253 2253 Size: 5326 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FspI TGC'GCA Cuts at: 3832 3832 Size: 5326 HaeII r_GCGC'y Cuts at: 1678 2116 2191 4473 1678 Size: 438 75 2282 2531 Fragments arranged by size: 2531 2282 438 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373 Size: 32 135 32 2395 32 42 32 2626 Fragments arranged by size: 2626 2395 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 2916 669 Size: 988 281 732 246 3079 Fragments arranged by size: 3079 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KasI G'GCGC_C Cuts at: 2187 2187 Size: 5326 KpnI G_GTAC'C Cuts at: 2651 2651 Size: 5326 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4440 4624 1305 Size: 3135 184 2007 Fragments arranged by size: 3135 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3504 Fragments arranged by size: 3504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2398 3284 3643 3802 518 Size: 648 1232 886 359 159 2042 Fragments arranged by size: 2042 1232 886 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2742 3419 4567 4812 1606 Size: 1136 677 1148 245 2120 Fragments arranged by size: 2120 1148 1136 677 245 NarI GG'CG_CC Cuts at: 2188 2188 Size: 5326 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3835 Fragments arranged by size: 3835 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2684 4229 1132 Size: 97 1455 1545 2229 Fragments arranged by size: 2229 1545 1455 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5326 PciI A'CATG_T Cuts at: 4225 4225 Size: 5326 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5326 PfoI T'CCnGG_A Cuts at: 2283 2409 2283 Size: 126 5200 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PmlI CAC'GTG Cuts at: 2396 2396 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2253 2253 Size: 5326 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PstI C_TGCA'G Cuts at: 830 2142 2678 830 Size: 1312 536 3478 Fragments arranged by size: 3478 1312 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SanDI GG'GwC_CC Cuts at: 2253 2253 Size: 5326 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2138 2574 2620 2674 3809 4490 4681 Size: 219 1093 436 46 54 1135 681 191 Cuts at: 4681 826 Size: 1471 Fragments arranged by size: 1471 1135 1093 681 436 219 191 54 46 SfoI GGC'GCC Cuts at: 2189 2189 Size: 5326 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2519 2653 1872 Size: 647 134 4545 Fragments arranged by size: 4545 647 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 3250 5 Size: 47 3198 2081 Fragments arranged by size: 3198 2081 47 StuI AGG'CCT Cuts at: 1517 2572 1517 Size: 1055 4271 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2347 2765 513 Size: 552 939 30 313 418 3074 Fragments arranged by size: 3074 939 552 418 313 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 3353 3370 3523 3708 4153 325 Size: 1255 587 1186 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1186 587 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2457 3572 5284 Size: 274 80 33 51 1443 480 1115 1712 Cuts at: 5284 96 Size: 138 Fragments arranged by size: 1712 1443 1115 480 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 5286 5318 2042 Size: 32 3212 32 2050 Fragments arranged by size: 3212 2050 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5326 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2517 2651 1870 Size: 647 134 4545 Fragments arranged by size: 4545 647 134 XmnI GAAnn'nnTTC Cuts at: 3455 3455 Size: 5326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3134 276 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI