Restriction map for
pFN21AE0569
##################################
# NO CUT ENZYMES ( 196 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MflI MluI
MluNI MroI MscI MspA1I MspI
MvaI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 125 GTAGAC GT^[AC][GT]AC
AciI 381 CCGC CCGC(-3/-1)
AcsI 475 GAATTT [GA]^AATT[CT]
AflIII 540 ACATGT A^C[GA][CT]GT
ApoI 475 GAATTT [GA]^AATT[CT]
AvaII 378 GGACC G^G[AT]CC
BbsI 3 GTCTTC GAAGAC(2/6)
BbvI 319 GCAGC GCAGC(8/12)
BpuAI 3 GTCTTC GAAGAC(2/6)
BseRI 8 CTCCTC GAGGAG(10/8)
BsiYI 169 CCTAGATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 169 CCTAGATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 377 GGGAC GGGAC(10/14)
BsmI 62 GCATTC GAATGC(1/-1)
BspLU11I 540 ACATGT A^CATGT
BsrI 199 ACTGG ACTGG(1/-1)
BstAPI 138 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 360 TTCGAA TT^CGAA
DpnII 240 GATC ^GATC
Fnu4HI 319 GCAGC GC^[ACGT]GC
HaeIII 167 GGCC GG^CC
HindIII 356 AAGCTT A^AGCTT
HphI 242 TCACC GGTGA(8/7)
ItaI 319 GCAGC GC^[ACGT]GC
MaeIII 519 GTTAC ^GT[ACGT]AC
MboI 240 GATC ^GATC
MslI 259 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 240 GATC ^GATC
NlaIV 377 GGGACC GG[ACGT]^[ACGT]CC
NspV 360 TTCGAA TT^CGAA
Sau3AI 240 GATC ^GATC
SfaNI 289 GATGC GCATC(5/9)
SfcI 332 CTATAG C^T[GA][CT]AG
SfuI 360 TTCGAA TT^CGAA
TaqI 361 TCGA T^CGA
TfiI 31 GATTC G^A[AT]TC
TseI 319 GCAGC G^C[AT]GC
TthHB8I 361 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 397 GTAC GT^AC
538 GTAC
AlwNI 151 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
368 CAGGTGCTG
BstF5I 99 GGATG GGATG(2/0)
489 GGATG
Cfr13I 166 GGGCC G^G[ACGT]CC
378 GGACC
FokI 99 GGATG GGATG(9/13)
489 GGATG
MfeI 221 CAATTG C^AATTG
248 CAATTG
MunI 221 CAATTG C^AATTG
248 CAATTG
MwoI 138 GCAGGATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
147 GCACCAGAAGC
NlaIII 311 CATG CATG^
541 CATG
NspI 310 ACATGC [GA]CATG^[CT]
540 ACATGT
PleI 44 GAGTC GAGTC(4/5)
496 GAGTC
RsaI 397 GTAC GT^AC
538 GTAC
Sau96I 166 GGGCC G^G[ACGT]CC
378 GGACC
TspRI 130 CAGTG CA[GC]TG[ACGT][ACGT]^
407 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BfaI DdeI HinfI MaeI
MboII MnlI MseI Tru9I Tsp509I