################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MfeI MflI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 332 GGTACC G^GTACC AccIII 162 TCCGGA T^CCGGA AlwI 673 GGATC GGATC(4/5) AlwNI 901 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1004 CCTCAGG CC^T[ACGT]AGG Asp718I 332 GGTACC G^GTACC AspHI 575 GAGCTC G[AT]GC[AT]^C AspI 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 332 GGTACC G^G[CT][GA]CC BbvCI 225 CCTCAGC CCTCAGC(-5/-2) BsaBI 484 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 162 TCCGGA [AT]^CCGG[AT] BseAI 162 TCCGGA T^CCGGA BsiHKAI 575 GAGCTC G[AT]GC[AT]^C BsmAI 1029 GTCTC GTCTC(1/5) BsmFI 132 GTCCC GGGAC(10/14) BspEI 162 TCCGGA T^CCGGA BspMI 798 GCAGGT ACCTGC(4/8) BsrBI 19 GAGCGG CCGCTC(-3/-3) BsrDI 619 GCAATG GCAATG(2/0) BsrI 709 CCAGT ACTGG(1/-1) BstEII 780 GGTTACC G^GT[ACGT]ACC BstPI 780 GGTTACC G^GT[ACGT]ACC Bsu36I 1004 CCTCAGG CC^T[ACGT]AGG DraI 654 TTTAAA TTT^AAA DraII 130 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 1052 CCACGG C^C[GA][CT]GG EaeI 45 CGGCCA [CT]^GGCC[GA] EarI 748 CTCTTC CTCTTC(1/4) Eco81I 1004 CCTCAGG CC^T[ACGT]AGG EcoO109I 130 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 780 GGTTACC G^GT[ACGT]ACC KpnI 332 GGTACC GGTAC^C Ksp632I 748 CTCTTC CTCTTC(1/4) MaeII 1086 ACGT A^CGT MamI 484 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 162 TCCGGA T^CCGGA PflFI 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 130 GGGTCCC [GA]G^G[AT]CC[CT] PstI 725 CTGCAG CTGCA^G PvuII 169 CAGCTG CAG^CTG SacI 575 GAGCTC GAGCT^C SapI 747 GCTCTTC GCTCTTC(1/4) TaqI 215 TCGA T^CGA Tth111I 283 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 215 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 108 CGCG CG^CG 153 CGCG AcsI 842 AAATTT [GA]^AATT[CT] 999 AAATTC ApoI 842 AAATTT [GA]^AATT[CT] 999 AAATTC Asp700I 395 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 583 GAAGTACTTC BbsI 689 GAAGAC GAAGAC(2/6) 1088 GTCTTC BcnI 127 CCGGG CC^[GC]GG 1013 CCGGG BfaI 910 CTAG C^TAG 958 CTAG BpmI 707 CTCCAG CTGGAG(16/14) 790 CTCCAG BpuAI 689 GAAGAC GAAGAC(2/6) 1088 GTCTTC BspHI 470 TCATGA T^CATGA 579 TCATGA BstUI 108 CGCG CG^CG 153 CGCG DpnII 674 GATC ^GATC 924 GATC EcoT14I 48 CCAAGG C^C[AT][AT]GG 373 CCAAGG HaeII 138 GGCGCT [GA]GCGC^[CT] 195 GGCGCT HphI 516 GGTGA GGTGA(8/7) 931 GGTGA MaeI 910 CTAG C^TAG 958 CTAG MboI 674 GATC ^GATC 924 GATC MslI 186 CACGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 858 CACCAAAGTG MspA1I 8 CAGCGG C[AC]G^C[GT]G 169 CAGCTG MvnI 108 CGCG CG^CG 153 CGCG NciI 127 CCGGG CC^[GC]GG 1013 CCGGG NdeII 674 GATC ^GATC 924 GATC RcaI 470 TCATGA T^CATGA 579 TCATGA Sau3AI 674 GATC ^GATC 924 GATC ScaI 585 AGTACT AGT^ACT 626 AGTACT SfcI 725 CTGCAG C^T[GA][CT]AG 992 CTGTAG StyI 48 CCAAGG C^C[AT][AT]GG 373 CCAAGG TfiI 306 GAATC G^A[AT]TC 484 GATTC Tsp45I 450 GTCAC ^GT[GC]AC 823 GTGAC XmnI 395 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 583 GAAGTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboII MnlI MseI MspI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI Tru9I TseI Tsp509I