################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaLI AscI AspEI AspI AvrII BbrPI BbvCI BcgI BclI BfrI BlnI BsiEI BsiWI BsmI BspLU11I BsrBI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI HincII HindII HpaI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SgrAI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 2666 GGTACC G^GTACC AccI 227 GTCTAC GT^[AC][GT]AC AccIII 2055 TCCGGA T^CCGGA AcyI 2355 GGCGCC G[GA]^CG[CT]C Aor51HI 116 AGCGCT AGC^GCT ApaI 2469 GGGCCC GGGCC^C AseI 508 ATTAAT AT^TAAT AsnI 508 ATTAAT AT^TAAT Asp718I 2666 GGTACC G^GTACC AspHI 160 GAGCTC G[AT]GC[AT]^C AviII 2531 TGCGCA TGC^GCA BalI 2301 TGGCCA TGG^CCA BamHI 1339 GGATCC G^GATCC BbeI 2355 GGCGCC GGCGC^C BsaHI 2355 GGCGCC G[GA]^CG[CT]C BseAI 2055 TCCGGA T^CCGGA BsiHKAI 160 GAGCTC G[AT]GC[AT]^C BsmBI 2548 GAGACG CGTCTC(1/5) Bsp1407I 696 TGTACA T^GTACA BspDI 1171 ATCGAT AT^CGAT BspEI 2055 TCCGGA T^CCGGA BspHI 2690 TCATGA T^CATGA BsrDI 877 GCAATG GCAATG(2/0) BsrGI 696 TGTACA T^GTACA BssSI 1187 CACGAG CACGAG(-5/-1) BstBI 1219 TTCGAA TT^CGAA ClaI 1171 ATCGAT AT^CGAT DraI 1768 TTTAAA TTT^AAA DrdI 2308 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 116 AGCGCT AGC^GCT FspI 2531 TGCGCA TGC^GCA Hin1I 2355 GGCGCC G[GA]^CG[CT]C KasI 2355 GGCGCC G^GCGCC KpnI 2666 GGTACC GGTAC^C KspI 2465 CCGCGG CCGC^GG MluNI 2301 TGGCCA TGG^CCA MroI 2055 TCCGGA T^CCGGA MscI 2301 TGGCCA TGG^CCA NarI 2355 GGCGCC GG^CGCC NspV 1219 TTCGAA TT^CGAA PaeR7I 635 CTCGAG C^TCGAG PshBI 508 ATTAAT AT^TAAT RcaI 2690 TCATGA T^CATGA SacI 160 GAGCTC GAGCT^C SacII 2465 CCGCGG CCGC^GG SapI 418 GAAGAGC GCTCTTC(1/4) SfoI 2355 GGCGCC GGC^GCC SfuI 1219 TTCGAA TT^CGAA SmaI 2490 CCCGGG CCC^GGG SnaBI 1421 TACGTA TAC^GTA SspBI 696 TGTACA T^GTACA StuI 1163 AGGCCT AGG^CCT XhoI 635 CTCGAG C^TCGAG XmaCI 2490 CCCGGG C^CCGGG XmaI 2490 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BciVI 913 GTATCC GTATCC(6/5) 1719 GTATCC BglII 47 AGATCT A^GATCT 2591 AGATCT BlpI 81 GCTCAGC GC^T[ACGT]AGC 1198 GCTGAGC Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC 1198 GCTGAGC BsaAI 1421 TACGTA [CT]AC^GT[GA] 2563 TACGTG BsaWI 1360 TCCGGT [AT]^CCGG[AT] 2055 TCCGGA BsgI 2696 CTGCAC GTGCAG(16/14) 76 GTGCAG BsrFI 2352 GCCGGC [GA]^CCGG[CT] 2388 GCCGGC BstF5I 189 GGATG GGATG(2/0) 1398 GGATG CelII 81 GCTCAGC GC^T[ACGT]AGC 1198 GCTGAGC Cfr10I 2352 GCCGGC [GA]^CCGG[CT] 2388 GCCGGC DraIII 1040 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1516 CACAAGGTG DsaI 1553 CCGTGG C^C[GA][CT]GG 2465 CCGCGG EaeI 168 TGGCCG [CT]^GGCC[GA] 2301 TGGCCA EcoT14I 470 CCTTGG C^C[AT][AT]GG 971 CCAAGG FokI 189 GGATG GGATG(9/13) 1398 GGATG HindIII 203 AAGCTT A^AGCTT 1304 AAGCTT NaeI 2352 GCCGGC GCC^GGC 2388 GCCGGC NgoMIV 2352 GCCGGC G^CCGGC 2388 GCCGGC PflMI 54 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 210 CCAGCTGATGG PpuMI 2018 GGGTCCC [GA]G^G[AT]CC[CT] 2493 GGGTCCC PstI 112 CTGCAG CTGCA^G 124 CTGCAG PvuII 130 CAGCTG CAG^CTG 211 CAGCTG SfcI 112 CTGCAG C^T[GA][CT]AG 124 CTGCAG StyI 470 CCTTGG C^C[AT][AT]GG 971 CCAAGG Van91I 54 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 210 CCAGCTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI