################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbsI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 248 GGTACC G^GTACC AccI 409 GTCGAC GT^[AC][GT]AC AfaI 249 GTAC GT^AC Alw44I 1089 GTGCAC G^TGCAC ApaLI 1089 GTGCAC G^TGCAC Asp718I 248 GGTACC G^GTACC BalI 1268 TGGCCA TGG^CCA BbeI 875 GGCGCC GGCGC^C BbvCI 1576 CCTCAGC CCTCAGC(-5/-2) BsaI 429 GGTCTC GGTCTC(1/5) BsmAI 430 GTCTC GTCTC(1/5) BsmI 284 GAATGC GAATGC(1/-1) BsrBI 43 GAGCGG CCGCTC(-3/-3) BsrFI 1060 GCCGGC [GA]^CCGG[CT] Cfr10I 1060 GCCGGC [GA]^CCGG[CT] DraIII 556 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 456 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 409 GTCGAC GT[CT]^[GA]AC HindII 409 GTCGAC GT[CT]^[GA]AC KasI 875 GGCGCC G^GCGCC KpnI 248 GGTACC GGTAC^C MluNI 1268 TGGCCA TGG^CCA MscI 1268 TGGCCA TGG^CCA NaeI 1060 GCCGGC GCC^GGC NarI 875 GGCGCC GG^CGCC NgoMIV 1060 GCCGGC G^CCGGC NotI 859 GCGGCCGC GC^GGCCGC NspI 1448 ACATGC [GA]CATG^[CT] PflMI 555 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 249 GTAC GT^AC SalI 409 GTCGAC G^TCGAC SbfI 92 CCTGCAGG CCTGCA^GG SfoI 875 GGCGCC GGC^GCC Sse8387I 92 CCTGCAGG CCTGCA^GG StuI 37 AGGCCT AGG^CCT Van91I 555 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 395 CCAAGGATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 177 GGCGTC G[GA]^CG[CT]C 875 GGCGCC AflIII 290 ACGTGT A^C[GA][CT]GT 782 ACGTGT AlwI 399 GGATC GGATC(4/5) 506 GGATC AspHI 440 GTGCTC G[AT]GC[AT]^C 1089 GTGCAC AvaII 28 GGACC G^G[AT]CC 1716 GGACC BcgI 647 GCACCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 894 GCAGCTGGTTCG BsaHI 177 GGCGTC G[GA]^CG[CT]C 875 GGCGCC BseRI 271 GAGGAG GAGGAG(10/8) 1252 GAGGAG BsgI 1409 CTGCAC GTGCAG(16/14) 231 GTGCAG BsiEI 626 CGGCCG CG[GA][CT]^CG 860 CGGCCG BsiHKAI 440 GTGCTC G[AT]GC[AT]^C 1089 GTGCAC BstAPI 138 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 736 GCAGCAGCTGC BstYI 399 GGATCT [GA]^GATC[CT] 506 GGATCT EagI 626 CGGCCG C^GGCCG 860 CGGCCG EclXI 626 CGGCCG C^GGCCG 860 CGGCCG Eco52I 626 CGGCCG C^GGCCG 860 CGGCCG EcoT14I 395 CCAAGG C^C[AT][AT]GG 1271 CCAAGG Hin1I 177 GGCGTC G[GA]^CG[CT]C 875 GGCGCC HinfI 274 GAGTC G^A[ACGT]TC 535 GACTC MflI 399 GGATCT [GA]^GATC[CT] 506 GGATCT MslI 289 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACCCCTGTG PleI 535 GACTC GAGTC(4/5) 274 GAGTC StyI 395 CCAAGG C^C[AT][AT]GG 1271 CCAAGG XhoII 399 GGATCT [GA]^GATC[CT] 506 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AluI AlwNI ApaI AvaI BanI BanII BbrPI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PmaCI PmlI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI