(Circular) (Six-base) MAPSORT of: pFN21AE2149.seq Check: 8614 from: 1 to: 6526 pFN21AE2149 6526 bp With 182 enzymes: SgfI * March 28, 2012 12:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2064 3160 3427 2064 Size: 1096 267 5163 Fragments arranged by size: 5163 1096 267 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2415 3869 1937 Size: 478 1454 4594 Fragments arranged by size: 4594 1454 478 Acc65I G'GTAC_C Cuts at: 2253 3847 2253 Size: 1594 4932 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2555 3219 4589 5973 6241 1632 Size: 710 213 664 1370 1384 268 1917 Fragments arranged by size: 1917 1384 1370 710 664 268 213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 2295 2787 5425 6402 1203 Size: 1092 492 2638 977 1327 Fragments arranged by size: 2638 1327 1092 977 492 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 5255 5255 Size: 6526 AleI CACnn'nnGTG Cuts at: 1166 2298 2843 1166 Size: 1132 545 4849 Fragments arranged by size: 4849 1132 545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6526 AlwNI CAG_nnn'CTG Cuts at: 2149 2747 3044 3242 3701 4269 5841 2149 Size: 598 297 198 459 568 1572 2834 Fragments arranged by size: 2834 1572 598 568 459 297 198 ApaI G_GGCC'C Cuts at: 2080 2088 3405 3679 2080 Size: 8 1317 274 4927 Fragments arranged by size: 4927 1317 274 8 ApaLI G'TGCA_C Cuts at: 3094 4583 5739 3094 Size: 1489 1156 3881 Fragments arranged by size: 3881 1489 1156 ApoI r'AATT_y Cuts at: 1493 3835 4061 4213 6322 1493 Size: 2342 226 152 2109 1697 Fragments arranged by size: 2342 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2177 2283 3481 3507 3851 1870 Size: 73 137 97 106 1198 26 344 4545 Fragments arranged by size: 4545 1198 344 137 106 97 73 26 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2245 2278 2245 Size: 33 6493 BamHI G'GATC_C Cuts at: 3856 4223 3856 Size: 367 6159 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 2123 2253 2837 2855 Size: 325 217 627 265 71 130 584 18 Cuts at: 2855 2880 3062 3511 3847 5302 618 Size: 25 182 449 336 1455 1842 Fragments arranged by size: 1842 1455 627 584 449 336 325 265 217 182 130 71 25 18 BanII G_rGCy'C Cuts at: 1391 2080 2088 2379 2675 2918 3186 3405 3679 Size: 689 8 291 296 243 268 219 274 Cuts at: 3679 3845 1391 Size: 166 4072 Fragments arranged by size: 4072 689 296 291 274 268 243 219 166 8 BbeI G_GCGC'C Cuts at: 2884 2884 Size: 6526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5555 BbvCI CC'TCA_GC Cuts at: 3582 3582 Size: 6526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2641 2675 2888 2922 4717 4751 1903 Size: 34 704 34 213 34 1795 34 3678 Fragments arranged by size: 3678 1795 704 213 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 1548 Size: 6526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 3347 3428 Size: 107 122 71 631 605 378 1297 81 Cuts at: 3428 3740 5137 136 Size: 312 1397 1525 Fragments arranged by size: 1525 1397 1297 631 605 378 312 122 107 81 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 2555 2706 2972 3243 3562 3943 2555 Size: 151 266 271 319 381 5138 Fragments arranged by size: 5138 381 319 271 266 151 Bme1580I G_kGCm'C Cuts at: 1790 2080 2088 2126 2178 2234 2787 3038 3098 Size: 290 8 38 52 56 553 251 60 Cuts at: 3098 3195 3222 3360 3405 3474 3516 3643 3679 Size: 97 27 138 45 69 42 127 36 Cuts at: 3679 4587 5743 1790 Size: 908 1156 2573 Fragments arranged by size: 2573 1156 908 553 290 251 138 127 97 69 60 56 52 45 42 38 36 27 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3347 3630 3730 Size: 416 53 83 399 24 1930 283 100 Cuts at: 3730 4257 5215 442 Size: 527 958 1753 Fragments arranged by size: 1930 1753 958 527 416 399 283 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3793 3793 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 2915 3147 3275 3560 3696 5186 1239 Size: 1373 303 232 128 285 136 1490 2579 Fragments arranged by size: 2579 1490 1373 303 285 232 136 128 Bpu10I CC'TnA_GC Cuts at: 2046 3582 3690 2046 Size: 1536 108 4882 Fragments arranged by size: 4882 1536 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4651 5516 5814 4651 Size: 865 298 5363 Fragments arranged by size: 5363 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2440 5189 882 Size: 617 941 2749 2219 Fragments arranged by size: 2749 2219 941 617 BsaAI yAC'GTr Cuts at: 493 1204 2173 2235 2266 2296 2788 4317 493 Size: 711 969 62 31 30 492 1529 2702 Fragments arranged by size: 2702 1529 969 711 492 62 31 30 BsaBI GATnn'nnATC Cuts at: 6520 6520 Size: 6526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 2881 4333 4715 Size: 53 83 186 936 650 698 1452 382 Cuts at: 4715 5350 275 Size: 635 1451 Fragments arranged by size: 1452 1451 936 698 650 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4959 5357 5631 5778 6189 4959 Size: 398 274 147 411 5296 Fragments arranged by size: 5296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2746 2776 3248 3278 3352 Size: 30 165 30 1343 30 472 30 74 Cuts at: 3352 3382 1178 Size: 30 4322 Fragments arranged by size: 4322 1343 472 165 74 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 3272 1186 Size: 1105 981 4440 Fragments arranged by size: 4440 1105 981 BseYI C'CCAG_C Cuts at: 2062 2775 2932 3282 3570 3608 3706 5729 6225 Size: 713 157 350 288 38 98 2023 496 Cuts at: 6225 2062 Size: 2363 Fragments arranged by size: 2363 2023 713 496 350 288 157 98 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2257 3399 1163 Size: 573 521 1142 4290 Fragments arranged by size: 4290 1142 573 521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2634 2868 3976 4737 5765 1540 Size: 275 186 633 234 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 633 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2449 3098 4587 4672 5743 2449 Size: 649 1489 85 1071 3232 Fragments arranged by size: 3232 1489 1071 649 85 BsmI GAATG_Cn' Cuts at: 2295 4037 4130 2295 Size: 1742 93 4691 Fragments arranged by size: 4691 1742 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2088 2126 2178 2234 2379 2449 Size: 399 290 8 38 52 56 145 70 Cuts at: 2449 2675 2787 2918 3038 3098 3186 3195 3222 Size: 226 112 131 120 60 88 9 27 Cuts at: 3222 3360 3405 3474 3516 3643 3679 3845 4587 Size: 138 45 69 42 127 36 166 742 Cuts at: 4587 4672 5743 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 290 226 166 145 138 131 127 120 112 88 85 70 69 60 56 52 45 42 38 36 27 9 8 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 2105 3160 3427 3807 3881 844 Size: 1220 41 1055 267 380 74 3489 Fragments arranged by size: 3489 1220 1055 380 267 74 41 BsrBI CCG'CTC Cuts at: 1394 1823 2050 4413 1394 Size: 429 227 2363 3507 Fragments arranged by size: 3507 2363 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 5021 5195 65 Size: 1183 3773 174 1396 Fragments arranged by size: 3773 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3065 5170 5357 1700 Size: 110 1255 2105 187 2869 Fragments arranged by size: 2869 2105 1255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 4586 5598 4586 Size: 1012 5514 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 2747 794 Size: 1355 598 4573 Fragments arranged by size: 4573 1355 598 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6526 BstYI r'GATC_y Cuts at: 1972 2404 2511 3856 4223 4608 4625 6077 6163 Size: 432 107 1345 367 385 17 1452 86 Cuts at: 6163 6175 6521 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1345 432 385 367 346 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2923 3396 3516 513 Size: 552 1858 473 120 3523 Fragments arranged by size: 3523 1858 552 473 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2342 3262 487 Size: 683 56 1116 920 3751 Fragments arranged by size: 3751 1116 920 683 56 BtsI GCAGTG_nn' Cuts at: 2859 4042 4836 4856 2859 Size: 1183 794 20 4529 Fragments arranged by size: 4529 1183 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3580 3615 529 Size: 35 3016 35 3440 Fragments arranged by size: 3440 3016 35 35 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DraIII CAC_nnn'GTG Cuts at: 2566 2566 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5533 809 Size: 507 4217 1802 Fragments arranged by size: 4217 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2631 2865 Size: 54 1425 50 275 18 192 609 234 Cuts at: 2865 3273 3429 3973 4862 6491 8 Size: 408 156 544 889 1629 43 Fragments arranged by size: 1629 1425 889 609 544 408 275 234 192 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2631 2865 3973 1537 Size: 275 819 234 1108 4090 Fragments arranged by size: 4090 1108 819 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 4456 1353 Size: 3103 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2046 2170 2669 5113 5487 5633 Size: 1064 138 457 124 499 2444 374 146 Cuts at: 5633 387 Size: 1280 Fragments arranged by size: 2444 1280 1064 499 457 374 146 138 124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2555 2612 2915 3147 3219 3275 Size: 393 710 213 57 303 232 72 56 Cuts at: 3275 3560 3696 4589 5186 5973 6241 1239 Size: 285 136 893 597 787 268 1524 Fragments arranged by size: 1524 893 787 710 597 393 303 285 268 232 213 136 72 57 56 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2465 Size: 6526 EcoO109I rG'GnC_Cy Cuts at: 2076 2077 2084 2085 2716 3401 3402 3612 3675 Size: 1 7 1 631 685 1 210 63 Cuts at: 3675 3676 2076 Size: 1 4926 Fragments arranged by size: 4926 685 631 210 63 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 6526 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 2884 3013 3134 3701 5673 1678 Size: 1206 129 121 567 1972 2531 Fragments arranged by size: 2531 1972 1206 567 129 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2981 3013 3040 3072 3248 Size: 32 135 32 1409 32 27 32 176 Cuts at: 3248 3280 5167 5199 5241 5273 1373 Size: 32 1887 32 42 32 2626 Fragments arranged by size: 2626 1887 1409 176 135 42 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 2416 3870 4116 669 Size: 988 281 478 1454 246 3079 Fragments arranged by size: 3079 1454 988 478 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KasI G'GCGC_C Cuts at: 2880 2880 Size: 6526 KpnI G_GTAC'C Cuts at: 2257 3851 2257 Size: 1594 4932 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3699 3804 5640 5824 1305 Size: 2394 105 1836 184 2007 Fragments arranged by size: 2394 2007 1836 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 3275 10 Size: 54 1425 343 1443 3261 Fragments arranged by size: 3261 1443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2843 4484 4843 5002 518 Size: 648 1132 545 1641 359 159 2042 Fragments arranged by size: 2042 1641 1132 648 545 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2508 2747 2902 2971 3203 3942 4619 Size: 422 480 239 155 69 232 739 677 Cuts at: 4619 5767 6012 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 739 677 480 422 245 239 232 155 69 NaeI GCC'GGC Cuts at: 3067 3067 Size: 6526 NarI GG'CG_CC Cuts at: 2881 2881 Size: 6526 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5974 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NgoMIV G'CCGG_C Cuts at: 3065 3065 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 2865 3973 2865 Size: 1108 5418 NspI r_CATG'y Cuts at: 1132 1229 3457 3884 5429 1132 Size: 97 2228 427 1545 2229 Fragments arranged by size: 2229 2228 1545 427 97 PasI CC'CwG_GG Cuts at: 1348 3644 1348 Size: 2296 4230 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2566 1108 Size: 1458 5068 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PmlI CAC'GTG Cuts at: 2235 2266 2296 2788 2235 Size: 31 30 492 5973 Fragments arranged by size: 5973 492 31 30 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 2076 2084 3401 3675 2076 Size: 8 1317 274 4927 Fragments arranged by size: 4927 1317 274 8 PstI C_TGCA'G Cuts at: 830 2102 2854 3009 3202 3381 3878 830 Size: 1272 752 155 193 179 497 3478 Fragments arranged by size: 3478 1272 752 497 193 179 155 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2747 2902 2971 3203 2747 Size: 155 69 232 6070 Fragments arranged by size: 6070 232 155 69 SalI G'TCGA_C Cuts at: 1936 2414 3868 1936 Size: 478 1454 4594 Fragments arranged by size: 4594 1454 478 SbfI CC_TGCA'GG Cuts at: 2102 3878 2102 Size: 1776 4750 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2098 2850 3005 3198 3377 3765 3874 Size: 219 1053 752 155 193 179 388 109 Cuts at: 3874 5009 5690 5881 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1053 752 681 388 219 193 191 179 155 109 SfoI GGC'GCC Cuts at: 2882 2882 Size: 6526 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 2082 2285 3483 3509 3853 1872 Size: 210 203 1198 26 344 4545 Fragments arranged by size: 4545 1198 344 210 203 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 4630 5531 5793 820 Size: 197 926 2687 901 262 1553 Fragments arranged by size: 2687 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 2044 1517 Size: 527 5999 StyI C'CwwG_G Cuts at: 513 1065 2400 3276 3965 513 Size: 552 1335 876 689 3074 Fragments arranged by size: 3074 1335 876 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2350 4553 4570 4723 4908 5353 325 Size: 1255 770 2203 17 153 185 445 1498 Fragments arranged by size: 2203 1498 1255 770 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4772 6484 96 Size: 274 80 33 51 1443 2795 1712 138 Fragments arranged by size: 2795 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 2746 2778 3352 3384 6486 6518 2280 Size: 32 434 32 574 32 3102 32 2288 Fragments arranged by size: 3102 2288 574 434 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6526 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 2407 Size: 6526 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 2080 2283 3481 3507 3851 1870 Size: 210 203 1198 26 344 4545 Fragments arranged by size: 4545 1198 344 210 203 26 XmnI GAAnn'nnTTC Cuts at: 4655 4655 Size: 6526 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI