(Linear) (Six-base) MAPSORT of: KIEE2149.seq Check: 910 from: 1 to: 1821 KIEE2149.orf With 182 enzymes: SgfI * March 22, 2012 15:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 59 1155 1422 1821 Size: 59 1096 267 399 Fragments arranged by size: 1096 399 267 59 AccI GT'mk_AC Cuts at: 0 410 1821 Size: 410 1411 Acc65I G'GTAC_C Cuts at: 0 248 1821 Size: 248 1573 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 550 1214 1821 Size: 337 213 664 607 Fragments arranged by size: 664 607 337 213 AflIII A'CryG_T Cuts at: 0 290 782 1821 Size: 290 492 1039 Fragments arranged by size: 1039 492 290 AleI CACnn'nnGTG Cuts at: 0 293 838 1821 Size: 293 545 983 Fragments arranged by size: 983 545 293 AlwNI CAG_nnn'CTG Cuts at: 0 144 742 1039 1237 1696 1821 Size: 144 598 297 198 459 125 Fragments arranged by size: 598 459 297 198 144 125 ApaI G_GGCC'C Cuts at: 0 75 83 1400 1674 1821 Size: 75 8 1317 274 147 Fragments arranged by size: 1317 274 147 75 8 ApaLI G'TGCA_C Cuts at: 0 1089 1821 Size: 1089 732 AvaI C'yCGr_G Cuts at: 0 75 172 278 1476 1502 1821 Size: 75 97 106 1198 26 319 Fragments arranged by size: 1198 319 106 97 75 26 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 240 273 1821 Size: 240 33 1548 Fragments arranged by size: 1548 240 33 BanI G'GyrC_C Cuts at: 0 47 118 248 832 850 875 1057 1506 Size: 47 71 130 584 18 25 182 449 Cuts at: 1506 1821 Size: 315 Fragments arranged by size: 584 449 315 182 130 71 47 25 18 BanII G_rGCy'C Cuts at: 0 75 83 374 670 913 1181 1400 1674 Size: 75 8 291 296 243 268 219 274 Cuts at: 1674 1821 Size: 147 Fragments arranged by size: 296 291 274 268 243 219 147 75 8 BbeI G_GCGC'C Cuts at: 0 879 1821 Size: 879 942 BbvCI CC'TCA_GC Cuts at: 0 1577 1821 Size: 1577 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 636 670 883 917 1821 Size: 636 34 213 34 904 Fragments arranged by size: 904 636 213 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 45 1342 1423 1735 1821 Size: 45 1297 81 312 86 Fragments arranged by size: 1297 312 86 81 45 BlpI GC'TnA_GC Cuts at: 0 550 701 967 1238 1557 1821 Size: 550 151 266 271 319 264 Fragments arranged by size: 550 319 271 266 264 151 Bme1580I G_kGCm'C Cuts at: 0 75 83 121 173 229 782 1033 1093 Size: 75 8 38 52 56 553 251 60 Cuts at: 1093 1190 1217 1355 1400 1469 1511 1638 1674 Size: 97 27 138 45 69 42 127 36 Cuts at: 1674 1821 Size: 147 Fragments arranged by size: 553 251 147 138 127 97 75 69 60 56 52 45 42 38 36 27 8 BmrI ACTGGGnnnn_n' Cuts at: 0 1342 1625 1725 1821 Size: 1342 283 100 96 Fragments arranged by size: 1342 283 100 96 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1788 1821 Size: 1788 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 910 1142 1270 1555 1691 1821 Size: 607 303 232 128 285 136 130 Fragments arranged by size: 607 303 285 232 136 130 128 Bpu10I CC'TnA_GC Cuts at: 0 41 1577 1685 1821 Size: 41 1536 108 136 Fragments arranged by size: 1536 136 108 41 BsaI GGTCTCn'nnnn_ Cuts at: 0 435 1821 Size: 435 1386 BsaAI yAC'GTr Cuts at: 0 168 230 261 291 783 1821 Size: 168 62 31 30 492 1038 Fragments arranged by size: 1038 492 168 62 31 30 BsaHI Gr'CG_yC Cuts at: 0 178 876 1821 Size: 178 698 945 Fragments arranged by size: 945 698 178 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 741 771 1243 1273 1347 1377 1821 Size: 741 30 472 30 74 30 444 Fragments arranged by size: 741 472 444 74 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 1267 1821 Size: 286 981 554 Fragments arranged by size: 981 554 286 BseYI C'CCAG_C Cuts at: 0 57 770 927 1277 1565 1603 1701 1821 Size: 57 713 157 350 288 38 98 120 Fragments arranged by size: 713 350 288 157 120 98 57 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 252 1394 1821 Size: 252 1142 427 Fragments arranged by size: 1142 427 252 BsiEI CG_ry'CG Cuts at: 0 629 863 1821 Size: 629 234 958 Fragments arranged by size: 958 629 234 BsiHKAI G_wGCw'C Cuts at: 0 444 1093 1821 Size: 444 649 728 Fragments arranged by size: 728 649 444 BsmI GAATG_Cn' Cuts at: 0 290 1821 Size: 290 1531 Bsp1286I G_dGCh'C Cuts at: 0 75 83 121 173 229 374 444 670 Size: 75 8 38 52 56 145 70 226 Cuts at: 670 782 913 1033 1093 1181 1190 1217 1355 Size: 112 131 120 60 88 9 27 138 Cuts at: 1355 1400 1469 1511 1638 1674 1821 Size: 45 69 42 127 36 147 Fragments arranged by size: 226 147 145 138 131 127 120 112 88 75 70 69 60 56 52 45 42 38 36 27 9 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 59 100 1155 1422 1802 1821 Size: 59 41 1055 267 380 19 Fragments arranged by size: 1055 380 267 59 41 19 BsrBI CCG'CTC Cuts at: 0 45 1821 Size: 45 1776 BsrFI r'CCGG_y Cuts at: 0 1060 1821 Size: 1060 761 BstAPI GCAn_nnn'nTGC Cuts at: 0 144 742 1821 Size: 144 598 1079 Fragments arranged by size: 1079 598 144 BstYI r'GATC_y Cuts at: 0 399 506 1821 Size: 399 107 1315 Fragments arranged by size: 1315 399 107 BtgI C'CryG_G Cuts at: 0 918 1391 1511 1821 Size: 918 473 120 310 Fragments arranged by size: 918 473 310 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 337 1257 1821 Size: 337 920 564 Fragments arranged by size: 920 564 337 BtsI GCAGTG_nn' Cuts at: 0 854 1821 Size: 854 967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1575 1610 1821 Size: 1575 35 211 Fragments arranged by size: 1575 211 35 DraIII CAC_nnn'GTG Cuts at: 0 561 1821 Size: 561 1260 EaeI y'GGCC_r Cuts at: 0 17 626 860 1268 1424 1821 Size: 17 609 234 408 156 397 Fragments arranged by size: 609 408 397 234 156 17 EagI C'GGCC_G Cuts at: 0 626 860 1821 Size: 626 234 961 Fragments arranged by size: 961 626 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 41 165 664 1821 Size: 41 124 499 1157 Fragments arranged by size: 1157 499 124 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 550 607 910 1142 1214 1270 1555 Size: 337 213 57 303 232 72 56 285 Cuts at: 1555 1691 1821 Size: 136 130 Fragments arranged by size: 337 303 285 232 213 136 130 72 57 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 460 1821 Size: 460 1361 EcoO109I rG'GnC_Cy Cuts at: 0 71 72 79 80 711 1396 1397 1607 Size: 71 1 7 1 631 685 1 210 Cuts at: 1607 1670 1671 1821 Size: 63 1 150 Fragments arranged by size: 685 631 210 150 71 63 7 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 450 1821 Size: 450 1371 HaeII r_GCGC'y Cuts at: 0 879 1008 1129 1696 1821 Size: 879 129 121 567 125 Fragments arranged by size: 879 567 129 125 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 976 1008 1035 1067 1243 1275 1821 Size: 976 32 27 32 176 32 546 Fragments arranged by size: 976 546 176 32 32 32 27 HincII GTy'rAC Cuts at: 0 411 1821 Size: 411 1410 KasI G'GCGC_C Cuts at: 0 875 1821 Size: 875 946 KpnI G_GTAC'C Cuts at: 0 252 1821 Size: 252 1569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1694 1799 1821 Size: 1694 105 22 Fragments arranged by size: 1694 105 22 MscI TGG'CCA Cuts at: 0 1270 1821 Size: 1270 551 MslI CAynn'nnrTG Cuts at: 0 293 838 1821 Size: 293 545 983 Fragments arranged by size: 983 545 293 MspA1I CmG'CkG Cuts at: 0 23 503 742 897 966 1198 1821 Size: 23 480 239 155 69 232 623 Fragments arranged by size: 623 480 239 232 155 69 23 NaeI GCC'GGC Cuts at: 0 1062 1821 Size: 1062 759 NarI GG'CG_CC Cuts at: 0 876 1821 Size: 876 945 NgoMIV G'CCGG_C Cuts at: 0 1060 1821 Size: 1060 761 NotI GC'GGCC_GC Cuts at: 0 860 1821 Size: 860 961 NspI r_CATG'y Cuts at: 0 1452 1821 Size: 1452 369 PasI CC'CwG_GG Cuts at: 0 1639 1821 Size: 1639 182 PflMI CCAn_nnn'nTGG Cuts at: 0 561 1821 Size: 561 1260 PmlI CAC'GTG Cuts at: 0 230 261 291 783 1821 Size: 230 31 30 492 1038 Fragments arranged by size: 1038 492 230 31 30 PspOMI G'GGCC_C Cuts at: 0 71 79 1396 1670 1821 Size: 71 8 1317 274 151 Fragments arranged by size: 1317 274 151 71 8 PstI C_TGCA'G Cuts at: 0 97 849 1004 1197 1376 1821 Size: 97 752 155 193 179 445 Fragments arranged by size: 752 445 193 179 155 97 PvuII CAG'CTG Cuts at: 0 742 897 966 1198 1821 Size: 742 155 69 232 623 Fragments arranged by size: 742 623 232 155 69 SalI G'TCGA_C Cuts at: 0 409 1821 Size: 409 1412 SbfI CC_TGCA'GG Cuts at: 0 97 1821 Size: 97 1724 SfcI C'TryA_G Cuts at: 0 93 845 1000 1193 1372 1760 1821 Size: 93 752 155 193 179 388 61 Fragments arranged by size: 752 388 193 179 155 93 61 SfoI GGC'GCC Cuts at: 0 877 1821 Size: 877 944 SmaI CCC'GGG Cuts at: 0 77 280 1478 1504 1821 Size: 77 203 1198 26 317 Fragments arranged by size: 1198 317 203 77 26 StuI AGG'CCT Cuts at: 0 39 1821 Size: 39 1782 StyI C'CwwG_G Cuts at: 0 395 1271 1821 Size: 395 876 550 Fragments arranged by size: 876 550 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 345 1821 Size: 345 1476 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 275 307 741 773 1347 1379 1821 Size: 275 32 434 32 574 32 442 Fragments arranged by size: 574 442 434 275 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 402 1821 Size: 402 1419 XmaI C'CCGG_G Cuts at: 0 75 278 1476 1502 1821 Size: 75 203 1198 26 319 Fragments arranged by size: 1198 319 203 75 26 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI AvaI BaeI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PflMI PmlI PspOMI PstI PvuII SalI SbfI SfcI SfoI SmaI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BamHI BbsI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DrdI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NcoI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI