Restriction map for
pFN21AE0530
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BfaI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MscI MslI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
TseI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 416 TCCGGA T^CCGGA
AciI 212 CCGC CCGC(-3/-1)
AfaI 309 GTAC GT^AC
AflII 229 CTTAAG C^TTAAG
AlwI 297 GATCC GGATC(4/5)
AlwNI 340 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 205 GTGCTC G[AT]GC[AT]^C
AvaI 361 CCCGGG C^[CT]CG[GA]G
BanI 196 GGTGCC G^G[CT][GA]CC
BanII 4 GGGCTC G[GA]GC[CT]^C
BfrI 229 CTTAAG C^TTAAG
BglI 382 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 6 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 6 GCTCAGC GC^T[ACGT]AGC
BsaWI 416 TCCGGA [AT]^CCGG[AT]
BseAI 416 TCCGGA T^CCGGA
BsiHKAI 205 GTGCTC G[AT]GC[AT]^C
BsmAI 115 GAGAC GTCTC(1/5)
BsmFI 365 GGGAC GGGAC(10/14)
BsmI 328 GAATGC GAATGC(1/-1)
BsoBI 361 CCCGGG C^[CT]CG[GA]G
BspEI 416 TCCGGA T^CCGGA
BspHI 246 TCATGA T^CATGA
BstAPI 24 GCAGTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 106 GGTCACC G^GT[ACGT]ACC
BstPI 106 GGTCACC G^GT[ACGT]ACC
BstYI 296 AGATCC [GA]^GATC[CT]
CelII 6 GCTCAGC GC^T[ACGT]AGC
DpnII 297 GATC ^GATC
DraII 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 72 CCATGG C^C[GA][CT]GG
EcoO109I 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 106 GGTCACC G^GT[ACGT]ACC
EcoT14I 72 CCATGG C^C[AT][AT]GG
EcoT22I 330 ATGCAT ATGCA^T
HinfI 117 GACTC G^A[ACGT]TC
MaeII 38 ACGT A^CGT
MaeIII 107 GTCAC ^GT[ACGT]AC
MboI 297 GATC ^GATC
MboII 234 GAAGA GAAGA(8/7)
MflI 296 AGATCC [GA]^GATC[CT]
MroI 416 TCCGGA T^CCGGA
NcoI 72 CCATGG C^CATGG
NdeII 297 GATC ^GATC
NsiI 330 ATGCAT ATGCA^T
NspI 279 GCATGC [GA]CATG^[CT]
PflMI 67 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 117 GACTC GAGTC(4/5)
PstI 346 CTGCAG CTGCA^G
RcaI 246 TCATGA T^CATGA
RsaI 309 GTAC GT^AC
Sau3AI 297 GATC ^GATC
SfcI 346 CTGCAG C^T[GA][CT]AG
SmaI 361 CCCGGG CCC^GGG
SmlI 229 CTTAAG C^T[CT][GA]AG
SphI 279 GCATGC GCATG^C
StyI 72 CCATGG C^C[AT][AT]GG
Tsp45I 107 GTCAC ^GT[GC]AC
TspRI 201 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 67 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 296 AGATCC [GA]^GATC[CT]
XmaCI 361 CCCGGG C^CCGGG
XmaI 361 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 410 AGCT AG^CT
446 AGCT
BcnI 361 CCCGG CC^[GC]GG
362 CCGGG
BmyI 4 GGGCTC G[AGT]GC[ACT]^C
205 GTGCTC
BsiYI 67 CCAGGCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
72 CCATGGGCAGG
BslI 67 CCAGGCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
72 CCATGGGCAGG
Bsp1286I 4 GGGCTC G[AGT]GC[ACT]^C
205 GTGCTC
Cac8I 279 GCATGC GC[ACGT]^[ACGT]GC
353 GCAAGC
Cfr13I 389 GGGCC G^G[ACGT]CC
402 GGCCC
DdeI 7 CTCAG C^T[ACGT]AG
176 CTGAG
HapII 362 CCGG C^CGG
417 CCGG
HpaII 362 CCGG C^CGG
417 CCGG
HphI 108 TCACC GGTGA(8/7)
209 TCACC
MspI 362 CCGG C^CGG
417 CCGG
NciI 361 CCCGG CC^[GC]GG
362 CCGGG
NlaIV 196 GGTGCC GG[ACGT]^[ACGT]CC
388 GGGGCC
Sau96I 389 GGGCC G^G[ACGT]CC
402 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BpmI BsaJI BssKI BstF5I BstNI
EcoRII FokI HaeIII MnlI MseI
MvaI MwoI NlaIII ScrFI SfaNI
Tru9I