(Circular) (Six-base) MAPSORT of: pFN21AE0530.seq Check: 4508 from: 1 to: 5167 pFN21AE0530 5167 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2977 278 Size: 53 83 186 936 1441 2468 Fragments arranged by size: 2468 1441 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2510 1937 Size: 573 4594 Acc65I G'GTAC_C Cuts at: 2488 2488 Size: 5167 AclI AA'CG_TT Cuts at: 1563 3294 3667 1563 Size: 1731 373 3063 Fragments arranged by size: 3063 1731 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 3230 4614 4882 1632 Size: 542 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1056 542 268 AflII C'TTAA_G Cuts at: 820 1017 2234 820 Size: 197 1217 3753 Fragments arranged by size: 3753 1217 197 AflIII A'CryG_T Cuts at: 1203 4066 5043 1203 Size: 2863 977 1327 Fragments arranged by size: 2863 1327 977 AgeI A'CCGG_T Cuts at: 3998 3998 Size: 5167 AhdI GACnn_n'nnGTC Cuts at: 3896 3896 Size: 5167 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5167 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5167 AlwNI CAG_nnn'CTG Cuts at: 2350 2910 4482 2350 Size: 560 1572 3035 Fragments arranged by size: 3035 1572 560 ApaLI G'TGCA_C Cuts at: 3224 4380 3224 Size: 1156 4011 ApoI r'AATT_y Cuts at: 1493 2476 2702 2854 4963 1493 Size: 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 226 152 AseI AT'TA_AT Cuts at: 160 1032 3721 160 Size: 872 2689 1606 Fragments arranged by size: 2689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5167 AvaI C'yCGr_G Cuts at: 1870 1943 2366 2492 1870 Size: 73 423 126 4545 Fragments arranged by size: 4545 423 126 73 BamHI G'GATC_C Cuts at: 2497 2864 2497 Size: 367 4800 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2488 3943 618 Size: 325 217 627 414 287 1455 1842 Fragments arranged by size: 1842 1455 627 414 325 287 217 BanII G_rGCy'C Cuts at: 1391 2013 2486 1391 Size: 622 473 4072 Fragments arranged by size: 4072 622 473 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3358 3392 1903 Size: 34 1421 34 3678 Fragments arranged by size: 3678 1421 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3059 4269 3059 Size: 1210 3957 BclI T'GATC_A Cuts at: 1548 1548 Size: 5167 BfrBI ATG'CAT Cuts at: 2337 2337 Size: 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 3778 136 Size: 107 122 71 631 605 721 1385 1525 Fragments arranged by size: 1525 1385 721 631 605 122 107 71 BglII A'GATC_T Cuts at: 5162 5162 Size: 5167 BlpI GC'TnA_GC Cuts at: 2012 2584 2012 Size: 572 4595 Bme1580I G_kGCm'C Cuts at: 1790 3228 4384 1790 Size: 1438 1156 2573 Fragments arranged by size: 2573 1438 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5167 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2898 3856 442 Size: 416 53 83 399 24 1481 958 1753 Fragments arranged by size: 1753 1481 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5167 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2339 2432 3827 1239 Size: 908 192 93 1395 2579 Fragments arranged by size: 2579 1395 908 192 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3292 4157 4455 3292 Size: 865 298 4004 Fragments arranged by size: 4004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3830 882 Size: 617 2331 2219 Fragments arranged by size: 2331 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2958 493 Size: 711 1754 2702 Fragments arranged by size: 2702 1754 711 BsaBI GATnn'nnATC Cuts at: 5161 5161 Size: 5167 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2974 3356 3991 275 Size: 53 83 186 936 1441 382 635 1451 Fragments arranged by size: 1451 1441 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 3600 3998 4272 4419 4830 2421 Size: 1179 398 274 147 411 2758 Fragments arranged by size: 2758 1179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 1178 Size: 30 165 30 1017 30 3895 Fragments arranged by size: 3895 1017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5167 BseYI C'CCAG_C Cuts at: 2452 4370 4866 2452 Size: 1918 496 2753 Fragments arranged by size: 2753 1918 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4594 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2617 3378 4406 1540 Size: 275 186 616 761 1028 2301 Fragments arranged by size: 2301 1028 761 616 275 186 BsiHKAI G_wGCw'C Cuts at: 2214 3228 3313 4384 2214 Size: 1014 85 1071 2997 Fragments arranged by size: 2997 1071 1014 85 BsmI GAATG_Cn' Cuts at: 2339 2678 2771 2339 Size: 339 93 4735 Fragments arranged by size: 4735 339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2214 2486 3228 3313 4384 1391 Size: 399 223 201 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 272 223 201 85 BspEI T'CCGG_A Cuts at: 2421 4830 2421 Size: 2409 2758 BspHI T'CATG_A Cuts at: 2251 3056 4786 2251 Size: 805 1730 2632 Fragments arranged by size: 2632 1730 805 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 844 Size: 1678 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3054 1394 Size: 429 1231 3507 Fragments arranged by size: 3507 1231 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3662 3836 65 Size: 1183 2414 174 1396 Fragments arranged by size: 2414 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3811 3998 1700 Size: 110 2001 187 2869 Fragments arranged by size: 2869 2001 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5167 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5167 BssSI C'ACGA_G Cuts at: 3227 4239 3227 Size: 1012 4155 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 794 Size: 1241 3926 BstBI TT'CG_AA Cuts at: 3971 3971 Size: 5167 BstEII G'GTnAC_C Cuts at: 1184 2111 1184 Size: 927 4240 BstYI r'GATC_y Cuts at: 1972 2301 2497 2864 3249 3266 4718 4804 4816 Size: 329 196 367 385 17 1452 86 12 Cuts at: 4816 5162 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 329 196 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2077 513 Size: 552 939 73 3603 Fragments arranged by size: 3603 939 552 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4428 Fragments arranged by size: 4428 683 56 BtsI GCAGTG_nn' Cuts at: 2683 3477 3497 2683 Size: 794 20 4353 Fragments arranged by size: 4353 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5167 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5132 DraI TTT'AAA Cuts at: 2471 2471 Size: 5167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4174 809 Size: 507 2858 1802 Fragments arranged by size: 2858 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2614 3503 5132 Size: 54 1425 50 275 18 784 889 1629 Cuts at: 5132 8 Size: 43 Fragments arranged by size: 1629 1425 889 784 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2614 1537 Size: 275 802 4090 Fragments arranged by size: 4090 802 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3097 1353 Size: 1744 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3754 4128 4274 387 Size: 1064 138 2165 374 146 1280 Fragments arranged by size: 2165 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2174 2339 2432 3230 3827 4614 Size: 393 515 27 165 93 798 597 787 Cuts at: 4614 4882 1239 Size: 268 1524 Fragments arranged by size: 1524 798 787 597 515 393 268 165 93 27 EcoO109I rG'GnC_Cy Cuts at: 2407 2407 Size: 5167 EcoRI G'AATT_C Cuts at: 2476 2854 2476 Size: 378 4789 FspI TGC'GCA Cuts at: 3673 3673 Size: 5167 HaeII r_GCGC'y Cuts at: 1678 4314 1678 Size: 2636 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 2420 2452 3808 Size: 32 135 32 775 32 41 32 1356 Cuts at: 3808 3840 3882 3914 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 775 135 42 41 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2511 2757 669 Size: 988 281 573 246 3079 Fragments arranged by size: 3079 988 573 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5167 HpaI GTT'AAC Cuts at: 2757 2757 Size: 5167 KpnI G_GTAC'C Cuts at: 2492 2492 Size: 5167 MfeI C'AATT_G Cuts at: 2766 2766 Size: 5167 MluI A'CGCG_T Cuts at: 5043 5043 Size: 5167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 4281 4465 1305 Size: 1158 1818 184 2007 Fragments arranged by size: 2007 1818 1158 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3345 Fragments arranged by size: 3345 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3125 3484 3643 518 Size: 648 1959 359 159 2042 Fragments arranged by size: 2042 1959 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2583 3260 4408 4653 1606 Size: 977 677 1148 245 2120 Fragments arranged by size: 2120 1148 977 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2077 513 Size: 552 939 73 3603 Fragments arranged by size: 3603 939 552 73 NdeI CA'TA_TG Cuts at: 387 387 Size: 5167 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5167 NotI GC'GGCC_GC Cuts at: 2614 2614 Size: 5167 NsiI A_TGCA'T Cuts at: 2339 2339 Size: 5167 NspI r_CATG'y Cuts at: 1132 1229 2288 2525 4070 1132 Size: 97 1059 237 1545 2229 Fragments arranged by size: 2229 1545 1059 237 97 PasI CC'CwG_GG Cuts at: 1348 2221 2389 1348 Size: 873 168 4126 Fragments arranged by size: 4126 873 168 PciI A'CATG_T Cuts at: 4066 4066 Size: 5167 PflMI CCAn_nnn'nTGG Cuts at: 1108 2078 1108 Size: 970 4197 PmeI GTTT'AAAC Cuts at: 2471 2471 Size: 5167 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3236 3268 4749 4781 3236 Size: 32 1481 32 3622 Fragments arranged by size: 3622 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5167 PsiI TTA'TAA Cuts at: 2737 2737 Size: 5167 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 5135 PstI C_TGCA'G Cuts at: 830 2355 2519 830 Size: 1525 164 3478 Fragments arranged by size: 3478 1525 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5167 SalI G'TCGA_C Cuts at: 1936 2509 1936 Size: 573 4594 SbfI CC_TGCA'GG Cuts at: 2519 2519 Size: 5167 ScaI AGT'ACT Cuts at: 3415 5127 3415 Size: 1712 3455 SfcI C'TryA_G Cuts at: 826 1045 2351 2515 3650 4331 4522 826 Size: 219 1306 164 1135 681 191 1471 Fragments arranged by size: 1471 1306 1135 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5167 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5167 SmaI CCC'GGG Cuts at: 1872 2368 2494 1872 Size: 496 126 4545 Fragments arranged by size: 4545 496 126 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 3271 4172 4434 820 Size: 197 926 291 1037 901 262 1553 Fragments arranged by size: 1553 1037 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 2958 493 Size: 2465 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5167 SphI G_CATG'C Cuts at: 1132 2288 2525 1132 Size: 1156 237 3774 Fragments arranged by size: 3774 1156 237 SspI AAT'ATT Cuts at: 5 52 3091 5 Size: 47 3039 2081 Fragments arranged by size: 3039 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5167 StyI C'CwwG_G Cuts at: 513 1065 2004 2077 2606 513 Size: 552 939 73 529 3074 Fragments arranged by size: 3074 939 552 529 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 2213 3194 3211 3364 3549 3994 Size: 1255 550 83 981 17 153 185 445 Cuts at: 3994 325 Size: 1498 Fragments arranged by size: 1498 1255 981 550 445 185 153 83 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3413 5125 96 Size: 274 80 33 51 1443 1436 1712 138 Fragments arranged by size: 1712 1443 1436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5127 5159 5127 Size: 32 5135 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5167 XbaI T'CTAG_A Cuts at: 2503 2503 Size: 5167 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5167 XmaI C'CCGG_G Cuts at: 1870 2366 2492 1870 Size: 496 126 4545 Fragments arranged by size: 4545 496 126 XmnI GAAnn'nnTTC Cuts at: 3296 3296 Size: 5167 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2975 276 Size: 53 83 186 936 1441 2468 Fragments arranged by size: 2468 1441 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI