################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI AvrII BbvCI BfrI BlnI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoT22I FseI HindIII HpaI MfeI MluI MunI NcoI NdeI NheI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 562 GGTACC G^GTACC AccI 1675 GTAGAC GT^[AC][GT]AC AflIII 3617 ACATGT A^C[GA][CT]GT Alw44I 981 GTGCAC G^TGCAC ApaLI 981 GTGCAC G^TGCAC Asp718I 562 GGTACC G^GTACC AspI 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3770 TGCGCA TGC^GCA BamHI 1878 GGATCC G^GATCC BbrPI 3217 CACGTG CAC^GTG BciVI 485 GTATCC GTATCC(6/5) BglII 1532 AGATCT A^GATCT BlpI 1135 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1135 GCTGAGC GC^T[ACGT]AGC BsaBI 408 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2661 CGTCTC CGTCTC(1/5) BsmI 2122 GAATGC GAATGC(1/-1) BspLU11I 3617 ACATGT A^CATGT BsrBI 3163 GAGCGG CCGCTC(-3/-3) BssSI 2331 CACGAG CACGAG(-5/-1) BstEII 1788 GGTCACC G^GT[ACGT]ACC BstPI 1788 GGTCACC G^GT[ACGT]ACC BstXI 749 CCAAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1135 GCTGAGC GC^T[ACGT]AGC EcoO65I 1788 GGTCACC G^GT[ACGT]ACC EcoRI 963 GAATTC G^AATTC EcoRV 115 GATATC GAT^ATC FspI 3770 TGCGCA TGC^GCA KpnI 562 GGTACC GGTAC^C KspI 74 CCGCGG CCGC^GG MamI 408 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 28 GCGGCCGC GC^GGCCGC PflFI 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3217 CACGTG CAC^GTG PmlI 3217 CACGTG CAC^GTG PvuII 1133 CAGCTG CAG^CTG SacII 74 CCGCGG CCGC^GG SmaI 419 CCCGGG CCC^GGG SspI 2821 AATATT AAT^ATT Tth111I 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 746 CCACCAAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 419 CCCGGG C^CCGGG XmaI 419 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 1053 GACGTC GACGT^C 2809 GACGTC Asp700I 1353 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3198 GAAGGGCTTC BalI 2 TGGCCA TGG^CCA 464 TGGCCA BbeI 70 GGCGCC GGCGC^C 1430 GGCGCC BclI 248 TGATCA T^GATCA 1100 TGATCA BglI 1551 GCCGTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2913 GCCAGATGGGC BsiEI 29 CGGCCG CG[GA][CT]^CG 2114 CGGCCG BspHI 245 TCATGA T^CATGA 3194 TCATGA BsrDI 1449 CATTGC GCAATG(2/0) 3083 GCAATG BsrFI 14 GCCGGC [GA]^CCGG[CT] 2116 GCCGGC BstAPI 983 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3385 GCAGAGATTGC Cfr10I 14 GCCGGC [GA]^CCGG[CT] 2116 GCCGGC EagI 29 CGGCCG C^GGCCG 2114 CGGCCG EclXI 29 CGGCCG C^GGCCG 2114 CGGCCG Eco52I 29 CGGCCG C^GGCCG 2114 CGGCCG FbaI 248 TGATCA T^GATCA 1100 TGATCA HgaI 2535 GACGC GACGC(5/10) 2660 GCGTC HincII 3139 GTCAAC GT[CT]^[GA]AC 3823 GTCAAC HindII 3139 GTCAAC GT[CT]^[GA]AC 3823 GTCAAC KasI 70 GGCGCC G^GCGCC 1430 GGCGCC MluNI 2 TGGCCA TGG^CCA 464 TGGCCA MscI 2 TGGCCA TGG^CCA 464 TGGCCA MseI 347 TTAA T^TAA 1499 TTAA NaeI 14 GCCGGC GCC^GGC 2116 GCCGGC NarI 70 GGCGCC GG^CGCC 1430 GGCGCC NgoMIV 14 GCCGGC G^CCGGC 2116 GCCGGC NspI 3617 ACATGT [GA]CATG^[CT] 3773 GCATGT PstI 2428 CTGCAG CTGCA^G 2694 CTGCAG RcaI 245 TCATGA T^CATGA 3194 TCATGA SacI 451 GAGCTC GAGCT^C 1066 GAGCTC SapI 3297 GAAGAGC GCTCTTC(1/4) 267 GCTCTTC SexAI 1964 ACCTGGT A^CC[AT]GGT 2050 ACCTGGT SfoI 70 GGCGCC GGC^GCC 1430 GGCGCC Tru9I 347 TTAA T^TAA 1499 TTAA XmnI 1353 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3198 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApoI AspEI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PflMI PleI PpuMI PshAI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII