(Circular) (Six-base) MAPSORT of: pFN21AE3464.seq Check: 6950 from: 1 to: 8815 pFN21AE3464 8815 bp With 182 enzymes: SgfI * March 28, 2012 12:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2781 5222 2781 Size: 2441 6374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3062 4818 6625 278 Size: 53 83 186 936 1526 1756 1807 2468 Fragments arranged by size: 2468 1807 1756 1526 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3681 6158 1937 Size: 1744 2477 4594 Fragments arranged by size: 4594 2477 1744 Acc65I G'GTAC_C Cuts at: 2567 6136 2567 Size: 3569 5246 AclI AA'CG_TT Cuts at: 1563 6942 7315 1563 Size: 5379 373 3063 Fragments arranged by size: 5379 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2836 3482 3944 4058 4098 4184 4527 Size: 689 515 646 462 114 40 86 343 Cuts at: 4527 4855 5321 5612 6878 8262 8530 1632 Size: 328 466 291 1266 1384 268 1917 Fragments arranged by size: 1917 1384 1266 689 646 515 466 462 343 328 291 268 114 86 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8618 AflIII A'CryG_T Cuts at: 1203 5622 7714 8691 1203 Size: 4419 2092 977 1327 Fragments arranged by size: 4419 2092 1327 977 AgeI A'CCGG_T Cuts at: 7646 7646 Size: 8815 AhdI GACnn_n'nnGTC Cuts at: 2640 4819 5626 7544 2640 Size: 2179 807 1918 3911 Fragments arranged by size: 3911 2179 1918 807 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4448 5573 1184 Size: 3264 1125 4426 Fragments arranged by size: 4426 3264 1125 AlwNI CAG_nnn'CTG Cuts at: 2859 2869 4162 5333 6558 8130 2859 Size: 10 1293 1171 1225 1572 3544 Fragments arranged by size: 3544 1572 1293 1225 1171 10 ApaLI G'TGCA_C Cuts at: 2986 6872 8028 2986 Size: 3886 1156 3773 Fragments arranged by size: 3886 3773 1156 ApoI r'AATT_y Cuts at: 1493 2968 3174 3506 5708 6124 6350 6502 8611 Size: 1475 206 332 2202 416 226 152 2109 Cuts at: 8611 1493 Size: 1697 Fragments arranged by size: 2202 2109 1697 1475 416 332 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 7369 160 Size: 872 6337 1606 Fragments arranged by size: 6337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8815 AvaI C'yCGr_G Cuts at: 1870 1943 2291 2424 4048 5025 5903 6140 1870 Size: 73 348 133 1624 977 878 237 4545 Fragments arranged by size: 4545 1624 977 878 348 237 133 73 BamHI G'GATC_C Cuts at: 3883 6145 6512 3883 Size: 2262 367 6186 Fragments arranged by size: 6186 2262 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2567 3435 5578 6136 Size: 325 217 627 288 492 868 2143 558 Cuts at: 6136 7591 618 Size: 1455 1842 Fragments arranged by size: 2143 1842 1455 868 627 558 492 325 288 217 BanII G_rGCy'C Cuts at: 1391 2292 2460 3075 4599 5066 6134 1391 Size: 901 168 615 1524 467 1068 4072 Fragments arranged by size: 4072 1524 1068 901 615 467 168 BbeI G_GCGC'C Cuts at: 2079 3439 2079 Size: 1360 7455 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3047 3470 3791 4002 4199 5144 5596 Size: 971 1148 423 321 211 197 945 452 Cuts at: 5596 928 Size: 4147 Fragments arranged by size: 4147 1148 971 945 452 423 321 211 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 4483 4517 4663 4697 7006 Size: 34 2375 34 137 34 146 34 2309 Cuts at: 7006 7040 1903 Size: 34 3678 Fragments arranged by size: 3678 2375 2309 146 137 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2501 6707 7917 2501 Size: 4206 1210 3399 Fragments arranged by size: 4206 3399 1210 BclI T'GATC_A Cuts at: 1548 2253 3105 1548 Size: 705 852 7258 Fragments arranged by size: 7258 852 705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3562 4924 7426 Size: 107 122 71 631 605 1890 1362 2502 Cuts at: 7426 136 Size: 1525 Fragments arranged by size: 2502 1890 1525 1362 631 605 122 107 71 BglII A'GATC_T Cuts at: 3537 8810 3537 Size: 5273 3542 BlpI GC'TnA_GC Cuts at: 3141 6232 3141 Size: 3091 5724 Bme1580I G_kGCm'C Cuts at: 1790 2990 3879 4293 4335 5952 6876 8032 1790 Size: 1200 889 414 42 1617 924 1156 2573 Fragments arranged by size: 2573 1617 1200 1156 924 889 414 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2081 3760 6546 Size: 416 53 83 399 24 664 1679 2786 Cuts at: 6546 7504 442 Size: 958 1753 Fragments arranged by size: 2786 1753 1679 958 664 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8815 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2179 2968 4198 2179 Size: 789 1230 6796 Fragments arranged by size: 6796 1230 789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 3346 4193 5840 5939 7475 1239 Size: 935 1172 847 1647 99 1536 2579 Fragments arranged by size: 2579 1647 1536 1172 935 847 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 3737 5822 6940 7805 8103 2372 Size: 1365 2085 1118 865 298 3084 Fragments arranged by size: 3084 2085 1365 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2220 2795 3251 3573 3755 4065 5114 Size: 617 721 575 456 322 182 310 1049 Cuts at: 5114 5289 7478 882 Size: 175 2189 2219 Fragments arranged by size: 2219 2189 1049 721 617 575 456 322 310 182 175 BsaAI yAC'GTr Cuts at: 493 1204 4798 5002 5224 6606 493 Size: 711 3594 204 222 1382 2702 Fragments arranged by size: 3594 2702 1382 711 222 204 BsaBI GATnn'nnATC Cuts at: 2417 8809 2417 Size: 6392 2423 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 3059 3436 4815 Size: 53 83 186 936 543 983 377 1379 Cuts at: 4815 6622 7004 7639 275 Size: 1807 382 635 1451 Fragments arranged by size: 1807 1451 1379 983 936 635 543 382 377 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 3231 4342 5538 5581 7248 7646 7920 8067 Size: 1107 1111 1196 43 1667 398 274 147 Cuts at: 8067 8478 2124 Size: 411 2461 Fragments arranged by size: 2461 1667 1196 1111 1107 411 398 274 147 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4045 4075 4169 4199 5912 Size: 30 165 30 2642 30 94 30 1713 Cuts at: 5912 5942 1178 Size: 30 4051 Fragments arranged by size: 4051 2642 1713 165 94 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2558 3135 3267 4190 4361 4664 5903 5927 Size: 1372 577 132 923 171 303 1239 24 Cuts at: 5927 5996 1186 Size: 69 4005 Fragments arranged by size: 4005 1372 1239 923 577 303 171 132 69 24 BseYI C'CCAG_C Cuts at: 4930 5053 5855 6013 8018 8514 4930 Size: 123 802 158 2005 496 5231 Fragments arranged by size: 5231 2005 802 496 158 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2671 2855 4196 4489 5834 1163 Size: 573 935 184 1341 293 1345 4144 Fragments arranged by size: 4144 1345 1341 935 573 293 184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 4122 6265 7026 8054 1540 Size: 275 186 36 2085 2143 761 1028 2301 Fragments arranged by size: 2301 2143 2085 1028 761 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2460 2990 3075 4353 6876 6961 8032 2460 Size: 530 85 1278 2523 85 1071 3243 Fragments arranged by size: 3243 2523 1278 1071 530 85 85 BsmI GAATG_Cn' Cuts at: 4133 6326 6419 4133 Size: 2193 93 6529 Fragments arranged by size: 6529 2193 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4672 4672 Size: 8815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2460 2990 3075 3879 4293 4335 Size: 399 502 168 530 85 804 414 42 Cuts at: 4335 4353 4599 5066 5952 6134 6876 6961 8032 Size: 18 246 467 886 182 742 85 1071 Cuts at: 8032 1391 Size: 2174 Fragments arranged by size: 2174 1071 886 804 742 530 502 467 414 399 246 182 168 85 85 42 18 BspEI T'CCGG_A Cuts at: 2124 3231 4342 8478 2124 Size: 1107 1111 4136 2461 Fragments arranged by size: 4136 2461 1111 1107 BspHI T'CATG_A Cuts at: 2250 5199 6704 8434 2250 Size: 2949 1505 1730 2631 Fragments arranged by size: 2949 2631 1730 1505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2781 2813 2877 4218 4242 5222 5841 5856 Size: 1937 32 64 1341 24 980 619 15 Cuts at: 5856 6170 844 Size: 314 3489 Fragments arranged by size: 3489 1937 1341 980 619 314 64 32 24 15 BsrBI CCG'CTC Cuts at: 1394 1823 5170 6702 1394 Size: 429 3347 1532 3507 Fragments arranged by size: 3507 3347 1532 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3453 5095 7310 7484 65 Size: 1183 2205 1642 2215 174 1396 Fragments arranged by size: 2215 2205 1642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 4121 7459 7646 1700 Size: 110 209 2102 3338 187 2869 Fragments arranged by size: 3338 2869 2102 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8815 BssSI C'ACGA_G Cuts at: 4336 6875 7887 4336 Size: 2539 1012 5264 Fragments arranged by size: 5264 2539 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2994 5396 794 Size: 2200 2402 4213 Fragments arranged by size: 4213 2402 2200 BstBI TT'CG_AA Cuts at: 7619 7619 Size: 8815 BstEII G'GTnAC_C Cuts at: 1184 3793 1184 Size: 2609 6206 BstXI CCAn_nnnn'nTGG Cuts at: 2761 2761 Size: 8815 BstYI r'GATC_y Cuts at: 1972 3537 3883 4158 6145 6512 6897 6914 8366 Size: 1565 346 275 1987 367 385 17 1452 Cuts at: 8366 8452 8464 8810 1972 Size: 86 12 346 1977 Fragments arranged by size: 1987 1977 1565 1452 385 367 346 346 275 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3308 3698 4458 3308 Size: 390 760 7665 Fragments arranged by size: 7665 760 390 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2502 4293 513 Size: 552 939 75 423 1791 5035 Fragments arranged by size: 5035 1791 939 552 423 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4278 487 Size: 683 56 3052 5024 Fragments arranged by size: 5024 3052 683 56 BtsI GCAGTG_nn' Cuts at: 2644 4477 6331 7125 7145 2644 Size: 1833 1854 794 20 4314 Fragments arranged by size: 4314 1854 1833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4738 4773 5250 5285 529 Size: 35 4174 35 477 35 4059 Fragments arranged by size: 4174 4059 477 35 35 35 DraI TTT'AAA Cuts at: 6119 6119 Size: 8815 DraIII CAC_nnn'GTG Cuts at: 2757 4219 5227 2757 Size: 1462 1008 6345 Fragments arranged by size: 6345 1462 1008 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7822 809 Size: 507 6506 1802 Fragments arranged by size: 6506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2034 2058 Size: 54 1425 50 275 18 177 27 24 Cuts at: 2058 2469 4119 4284 5324 5347 6262 7151 8780 Size: 411 1650 165 1040 23 915 889 1629 Cuts at: 8780 8 Size: 43 Fragments arranged by size: 1650 1629 1425 1040 915 889 411 275 177 165 54 50 43 27 24 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 4119 6262 1537 Size: 275 222 2085 2143 4090 Fragments arranged by size: 4090 2143 2085 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 3368 4874 5297 6745 1353 Size: 926 1089 1506 423 1448 3423 Fragments arranged by size: 3423 1506 1448 1089 926 423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3185 4007 6097 7402 7776 7922 Size: 1064 138 1596 822 2090 1305 374 146 Cuts at: 7922 387 Size: 1280 Fragments arranged by size: 2090 1596 1305 1280 1064 822 374 146 138 EcoICRI GAG'CTC Cuts at: 2458 3073 2458 Size: 615 8200 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2321 2836 3346 3482 3944 4058 Size: 393 542 147 515 510 136 462 114 Cuts at: 4058 4098 4184 4193 4527 4855 5321 5612 5840 Size: 40 86 9 334 328 466 291 228 Cuts at: 5840 5939 6878 7475 8262 8530 1239 Size: 99 939 597 787 268 1524 Fragments arranged by size: 1524 939 787 597 542 515 510 466 462 393 334 328 291 268 228 147 136 114 99 86 40 9 EcoNI CCTnn'n_nnAGG Cuts at: 4186 5186 5679 5837 4186 Size: 1000 493 158 7164 Fragments arranged by size: 7164 1000 493 158 EcoO109I rG'GnC_Cy Cuts at: 2305 2866 3002 3613 4053 4254 5443 6006 2305 Size: 561 136 611 440 201 1189 563 5114 Fragments arranged by size: 5114 1189 611 563 561 440 201 136 EcoRI G'AATT_C Cuts at: 2968 6124 6502 2968 Size: 3156 378 5281 Fragments arranged by size: 5281 3156 378 EcoRV GAT'ATC Cuts at: 2122 2122 Size: 8815 FspI TGC'GCA Cuts at: 5777 7321 5777 Size: 1544 7271 FspAI rTGC'GCAy Cuts at: 5777 5777 Size: 8815 HaeII r_GCGC'y Cuts at: 1678 2079 2842 3439 7962 1678 Size: 401 763 597 4523 2531 Fragments arranged by size: 4523 2531 763 597 401 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2626 2658 3072 3104 3467 Size: 32 135 32 1054 32 414 32 363 Cuts at: 3467 3499 3701 3733 3765 3875 3907 4045 4077 Size: 32 202 32 32 110 32 138 32 Cuts at: 4077 4138 4170 4805 4837 5019 5051 5612 5644 Size: 61 32 635 32 182 32 561 32 Cuts at: 5644 5948 5980 5990 6022 6082 6114 7456 7488 Size: 304 32 10 32 60 32 1342 32 Cuts at: 7488 7530 7562 1373 Size: 42 32 2626 Fragments arranged by size: 2626 1342 1054 635 561 414 363 304 202 182 138 135 110 61 60 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 5146 5830 6159 6405 669 Size: 988 281 3208 684 329 246 3079 Fragments arranged by size: 3208 3079 988 684 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8815 HpaI GTT'AAC Cuts at: 6405 6405 Size: 8815 KasI G'GCGC_C Cuts at: 2075 3435 2075 Size: 1360 7455 KpnI G_GTAC'C Cuts at: 2571 6140 2571 Size: 3569 5246 MfeI C'AATT_G Cuts at: 6414 6414 Size: 8815 MluI A'CGCG_T Cuts at: 8691 8691 Size: 8815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2612 3650 3687 3729 3966 7929 8113 1305 Size: 1307 1038 37 42 237 3963 184 2007 Fragments arranged by size: 3963 2007 1307 1038 237 184 42 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2471 10 Size: 54 1425 343 177 462 6354 Fragments arranged by size: 6354 1425 462 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3779 5777 6056 6773 7132 7291 518 Size: 648 2613 1998 279 717 359 159 2042 Fragments arranged by size: 2613 2042 1998 717 648 359 279 159 MspA1I CmG'CkG Cuts at: 1606 2044 2081 3140 4930 6231 6908 8056 8301 Size: 438 37 1059 1790 1301 677 1148 245 Cuts at: 8301 1606 Size: 2120 Fragments arranged by size: 2120 1790 1301 1148 1059 677 438 245 37 NaeI GCC'GGC Cuts at: 2021 4123 2021 Size: 2102 6713 NarI GG'CG_CC Cuts at: 2076 3436 2076 Size: 1360 7455 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7324 Fragments arranged by size: 7324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8815 NgoMIV G'CCGG_C Cuts at: 2019 4121 2019 Size: 2102 6713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8815 NotI GC'GGCC_GC Cuts at: 2034 6262 2034 Size: 4228 4587 NspI r_CATG'y Cuts at: 1132 1229 5626 5782 6173 7718 1132 Size: 97 4397 156 391 1545 2229 Fragments arranged by size: 4397 2229 1545 391 156 97 PasI CC'CwG_GG Cuts at: 1348 3731 1348 Size: 2383 6432 PciI A'CATG_T Cuts at: 5622 7714 5622 Size: 2092 6723 PflMI CCAn_nnn'nTGG Cuts at: 1108 3409 3669 3970 5224 1108 Size: 2301 260 301 1254 4699 Fragments arranged by size: 4699 2301 1254 301 260 PfoI T'CCnGG_A Cuts at: 2493 2829 5817 2493 Size: 336 2988 5491 Fragments arranged by size: 5491 2988 336 PmeI GTTT'AAAC Cuts at: 6119 6119 Size: 8815 PmlI CAC'GTG Cuts at: 5224 5224 Size: 8815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2385 2417 5858 5890 6884 6916 8397 8429 2385 Size: 32 3441 32 994 32 1481 32 2771 Fragments arranged by size: 3441 2771 1481 994 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2866 3002 4053 4254 5443 6006 2305 Size: 561 136 1051 201 1189 563 5114 Fragments arranged by size: 5114 1189 1051 563 561 201 136 PshAI GACnn'nnGTC Cuts at: 1613 2559 3791 5748 1613 Size: 946 1232 1957 4680 Fragments arranged by size: 4680 1957 1232 946 PsiI TTA'TAA Cuts at: 6385 6385 Size: 8815 PspXI vC'TCGA_Gb Cuts at: 4048 4048 Size: 8815 PstI C_TGCA'G Cuts at: 830 4437 4703 6167 830 Size: 3607 266 1464 3478 Fragments arranged by size: 3607 3478 1464 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8815 PvuII CAG'CTG Cuts at: 3140 3140 Size: 8815 SacI G_AGCT'C Cuts at: 2460 3075 2460 Size: 615 8200 SacII CC_GC'GG Cuts at: 2082 2082 Size: 8815 SalI G'TCGA_C Cuts at: 1936 6157 1936 Size: 4221 4594 SanDI GG'GwC_CC Cuts at: 3002 3002 Size: 8815 SapI GCTCTTCn'nnn_ Cuts at: 2279 5297 2279 Size: 3018 5797 SbfI CC_TGCA'GG Cuts at: 6167 6167 Size: 8815 ScaI AGT'ACT Cuts at: 7063 8775 7063 Size: 1712 7103 SexAI A'CCwGG_T Cuts at: 3969 4055 3969 Size: 86 8729 SfcI C'TryA_G Cuts at: 826 1045 4433 4699 4729 6163 7298 7979 8170 Size: 219 3388 266 30 1434 1135 681 191 Cuts at: 8170 826 Size: 1471 Fragments arranged by size: 3388 1471 1434 1135 681 266 219 191 30 SfoI GGC'GCC Cuts at: 2077 3437 2077 Size: 1360 7455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8815 SmaI CCC'GGG Cuts at: 1872 2426 6142 1872 Size: 554 3716 4545 Fragments arranged by size: 4545 3716 554 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 2387 3752 4048 5025 5837 Size: 197 926 348 96 1365 296 977 812 Cuts at: 5837 6919 7820 8082 820 Size: 1082 901 262 1553 Fragments arranged by size: 1553 1365 1082 977 926 901 812 348 296 262 197 96 SnaBI TAC'GTA Cuts at: 493 6606 493 Size: 6113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8815 SphI G_CATG'C Cuts at: 1132 6173 1132 Size: 5041 3774 SspI AAT'ATT Cuts at: 5 52 4828 6739 5 Size: 47 4776 1911 2081 Fragments arranged by size: 4776 2081 1911 47 StuI AGG'CCT Cuts at: 1517 2700 4357 5192 5685 1517 Size: 1183 1657 835 493 4647 Fragments arranged by size: 4647 1657 1183 835 493 StyI C'CwwG_G Cuts at: 513 1065 2004 3012 3261 3291 3429 4258 4512 Size: 552 939 1008 249 30 138 829 254 Cuts at: 4512 5250 5680 6254 513 Size: 738 430 574 3074 Fragments arranged by size: 3074 1008 939 829 738 574 552 430 254 249 138 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3744 3977 5185 6090 6842 6859 7012 Size: 1255 2164 233 1208 905 752 17 153 Cuts at: 7012 7197 7642 325 Size: 185 445 1498 Fragments arranged by size: 2164 1498 1255 1208 905 752 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7061 8773 96 Size: 274 80 33 51 1443 5084 1712 138 Fragments arranged by size: 5084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2809 8775 8807 2777 Size: 32 5966 32 2785 Fragments arranged by size: 5966 2785 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4057 1316 Size: 2741 6074 XbaI T'CTAG_A Cuts at: 6151 6151 Size: 8815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 2758 Size: 8815 XhoI C'TCGA_G Cuts at: 1943 2291 4048 5025 1943 Size: 348 1757 977 5733 Fragments arranged by size: 5733 1757 977 348 XmaI C'CCGG_G Cuts at: 1870 2424 6140 1870 Size: 554 3716 4545 Fragments arranged by size: 4545 3716 554 XmnI GAAnn'nnTTC Cuts at: 3362 5207 6944 3362 Size: 1845 1737 5233 Fragments arranged by size: 5233 1845 1737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3060 4816 6623 276 Size: 53 83 186 936 1526 1756 1807 2468 Fragments arranged by size: 2468 1807 1756 1526 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I FalI FseI NruI NsiI PacI PspOMI PsrI RsrII SfiI SrfI SwaI