(Linear) (Six-base) MAPSORT of: KIEE3464.seq Check: 6520 from: 1 to: 4110 KIEE3464.orf With 182 enzymes: SgfI * March 22, 2012 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 776 3217 4110 Size: 776 2441 893 Fragments arranged by size: 2441 893 776 AatII G_ACGT'C Cuts at: 0 1057 2813 4110 Size: 1057 1756 1297 Fragments arranged by size: 1756 1297 1057 AccI GT'mk_AC Cuts at: 0 1676 4110 Size: 1676 2434 Acc65I G'GTAC_C Cuts at: 0 562 4110 Size: 562 3548 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 831 1477 1939 2053 2093 2179 2522 Size: 316 515 646 462 114 40 86 343 Cuts at: 2522 2850 3316 3607 4110 Size: 328 466 291 503 Fragments arranged by size: 646 515 503 466 462 343 328 316 291 114 86 40 AflIII A'CryG_T Cuts at: 0 3617 4110 Size: 3617 493 AhdI GACnn_n'nnGTC Cuts at: 0 635 2814 3621 4110 Size: 635 2179 807 489 Fragments arranged by size: 2179 807 635 489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2443 3568 4110 Size: 2443 1125 542 Fragments arranged by size: 2443 1125 542 AlwNI CAG_nnn'CTG Cuts at: 0 854 864 2157 3328 4110 Size: 854 10 1293 1171 782 Fragments arranged by size: 1293 1171 854 782 10 ApaLI G'TGCA_C Cuts at: 0 981 4110 Size: 981 3129 ApoI r'AATT_y Cuts at: 0 963 1169 1501 3703 4110 Size: 963 206 332 2202 407 Fragments arranged by size: 2202 963 407 332 206 AvaI C'yCGr_G Cuts at: 0 286 419 2043 3020 3898 4110 Size: 286 133 1624 977 878 212 Fragments arranged by size: 1624 977 878 286 212 133 BamHI G'GATC_C Cuts at: 0 1878 4110 Size: 1878 2232 BanI G'GyrC_C Cuts at: 0 70 562 1430 3573 4110 Size: 70 492 868 2143 537 Fragments arranged by size: 2143 868 537 492 70 BanII G_rGCy'C Cuts at: 0 287 455 1070 2594 3061 4110 Size: 287 168 615 1524 467 1049 Fragments arranged by size: 1524 1049 615 467 287 168 BbeI G_GCGC'C Cuts at: 0 74 1434 4110 Size: 74 1360 2676 Fragments arranged by size: 2676 1360 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 1042 1465 1786 1997 2194 3139 3591 4110 Size: 1042 423 321 211 197 945 452 519 Fragments arranged by size: 1042 945 519 452 423 321 211 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2307 2341 2478 2512 2658 2692 4110 Size: 2307 34 137 34 146 34 1418 Fragments arranged by size: 2307 1418 146 137 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 496 4110 Size: 496 3614 BclI T'GATC_A Cuts at: 0 248 1100 4110 Size: 248 852 3010 Fragments arranged by size: 3010 852 248 BglI GCCn_nnn'nGGC Cuts at: 0 1557 2919 4110 Size: 1557 1362 1191 Fragments arranged by size: 1557 1362 1191 BglII A'GATC_T Cuts at: 0 1532 4110 Size: 1532 2578 BlpI GC'TnA_GC Cuts at: 0 1136 4110 Size: 1136 2974 Bme1580I G_kGCm'C Cuts at: 0 985 1874 2288 2330 3947 4110 Size: 985 889 414 42 1617 163 Fragments arranged by size: 1617 985 889 414 163 42 BmrI ACTGGGnnnn_n' Cuts at: 0 76 1755 4110 Size: 76 1679 2355 Fragments arranged by size: 2355 1679 76 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 174 963 2193 4110 Size: 174 789 1230 1917 Fragments arranged by size: 1917 1230 789 174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 1341 2188 3835 3934 4110 Size: 169 1172 847 1647 99 176 Fragments arranged by size: 1647 1172 847 176 169 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 1732 3817 4110 Size: 367 1365 2085 293 Fragments arranged by size: 2085 1365 367 293 BsaI GGTCTCn'nnnn_ Cuts at: 0 215 790 1246 1568 1750 2060 3109 3284 Size: 215 575 456 322 182 310 1049 175 Cuts at: 3284 4110 Size: 826 Fragments arranged by size: 1049 826 575 456 322 310 215 182 175 BsaAI yAC'GTr Cuts at: 0 2793 2997 3219 4110 Size: 2793 204 222 891 Fragments arranged by size: 2793 891 222 204 BsaBI GATnn'nnATC Cuts at: 0 412 4110 Size: 412 3698 BsaHI Gr'CG_yC Cuts at: 0 71 1054 1431 2810 4110 Size: 71 983 377 1379 1300 Fragments arranged by size: 1379 1300 983 377 71 BsaWI w'CCGG_w Cuts at: 0 119 1226 2337 3533 3576 4110 Size: 119 1107 1111 1196 43 534 Fragments arranged by size: 1196 1111 1107 534 119 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2040 2070 2164 2194 3907 3937 4110 Size: 2040 30 94 30 1713 30 173 Fragments arranged by size: 2040 1713 173 94 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 553 1130 1262 2185 2356 2659 3898 3922 Size: 553 577 132 923 171 303 1239 24 Cuts at: 3922 3991 4110 Size: 69 119 Fragments arranged by size: 1239 923 577 553 303 171 132 119 69 24 BseYI C'CCAG_C Cuts at: 0 2925 3048 3850 4008 4110 Size: 2925 123 802 158 102 Fragments arranged by size: 2925 802 158 123 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 666 850 2191 2484 3829 4110 Size: 666 184 1341 293 1345 281 Fragments arranged by size: 1345 1341 666 293 281 184 BsiEI CG_ry'CG Cuts at: 0 32 2117 4110 Size: 32 2085 1993 Fragments arranged by size: 2085 1993 32 BsiHKAI G_wGCw'C Cuts at: 0 455 985 1070 2348 4110 Size: 455 530 85 1278 1762 Fragments arranged by size: 1762 1278 530 455 85 BsmI GAATG_Cn' Cuts at: 0 2128 4110 Size: 2128 1982 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2667 4110 Size: 2667 1443 Bsp1286I G_dGCh'C Cuts at: 0 287 455 985 1070 1874 2288 2330 2348 Size: 287 168 530 85 804 414 42 18 Cuts at: 2348 2594 3061 3947 4110 Size: 246 467 886 163 Fragments arranged by size: 886 804 530 467 414 287 246 168 163 85 42 18 BspEI T'CCGG_A Cuts at: 0 119 1226 2337 4110 Size: 119 1107 1111 1773 Fragments arranged by size: 1773 1111 1107 119 BspHI T'CATG_A Cuts at: 0 245 3194 4110 Size: 245 2949 916 Fragments arranged by size: 2949 916 245 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 776 808 872 2213 2237 3217 3836 3851 Size: 776 32 64 1341 24 980 619 15 Cuts at: 3851 4110 Size: 259 Fragments arranged by size: 1341 980 776 619 259 64 32 24 15 BsrBI CCG'CTC Cuts at: 0 3165 4110 Size: 3165 945 BsrDI GCAATG_nn' Cuts at: 0 1448 3090 4110 Size: 1448 1642 1020 Fragments arranged by size: 1642 1448 1020 BsrFI r'CCGG_y Cuts at: 0 14 2116 4110 Size: 14 2102 1994 Fragments arranged by size: 2102 1994 14 BssSI C'ACGA_G Cuts at: 0 2331 4110 Size: 2331 1779 BstAPI GCAn_nnn'nTGC Cuts at: 0 989 3391 4110 Size: 989 2402 719 Fragments arranged by size: 2402 989 719 BstEII G'GTnAC_C Cuts at: 0 1788 4110 Size: 1788 2322 BstXI CCAn_nnnn'nTGG Cuts at: 0 756 4110 Size: 756 3354 BstYI r'GATC_y Cuts at: 0 1532 1878 2153 4110 Size: 1532 346 275 1957 Fragments arranged by size: 1957 1532 346 275 Bsu36I CC'TnA_GG Cuts at: 0 1303 1693 2453 4110 Size: 1303 390 760 1657 Fragments arranged by size: 1657 1303 760 390 BtgI C'CryG_G Cuts at: 0 74 497 2288 4110 Size: 74 423 1791 1822 Fragments arranged by size: 1822 1791 423 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2273 4110 Size: 2273 1837 BtsI GCAGTG_nn' Cuts at: 0 639 2472 4110 Size: 639 1833 1638 Fragments arranged by size: 1833 1638 639 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2733 2768 3245 3280 4110 Size: 2733 35 477 35 830 Fragments arranged by size: 2733 830 477 35 35 DraIII CAC_nnn'GTG Cuts at: 0 752 2214 3222 4110 Size: 752 1462 1008 888 Fragments arranged by size: 1462 1008 888 752 EaeI y'GGCC_r Cuts at: 0 2 29 53 464 2114 2279 3319 3342 Size: 2 27 24 411 1650 165 1040 23 Cuts at: 3342 4110 Size: 768 Fragments arranged by size: 1650 1040 768 411 165 27 24 23 2 EagI C'GGCC_G Cuts at: 0 29 2114 4110 Size: 29 2085 1996 Fragments arranged by size: 2085 1996 29 EarI CTCTTCn'nnn_ Cuts at: 0 274 1363 2869 3292 4110 Size: 274 1089 1506 423 818 Fragments arranged by size: 1506 1089 818 423 274 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1180 2002 4092 4110 Size: 1180 822 2090 18 Fragments arranged by size: 2090 1180 822 18 EcoICRI GAG'CTC Cuts at: 0 453 1068 4110 Size: 453 615 3042 Fragments arranged by size: 3042 615 453 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 316 831 1341 1477 1939 2053 2093 Size: 169 147 515 510 136 462 114 40 Cuts at: 2093 2179 2188 2522 2850 3316 3607 3835 3934 Size: 86 9 334 328 466 291 228 99 Cuts at: 3934 4110 Size: 176 Fragments arranged by size: 515 510 466 462 334 328 291 228 176 169 147 136 114 99 86 40 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 2181 3181 3674 3832 4110 Size: 2181 1000 493 158 278 Fragments arranged by size: 2181 1000 493 278 158 EcoO109I rG'GnC_Cy Cuts at: 0 300 861 997 1608 2048 2249 3438 4001 Size: 300 561 136 611 440 201 1189 563 Cuts at: 4001 4110 Size: 109 Fragments arranged by size: 1189 611 563 561 440 300 201 136 109 EcoRI G'AATT_C Cuts at: 0 963 4110 Size: 963 3147 EcoRV GAT'ATC Cuts at: 0 117 4110 Size: 117 3993 FspI TGC'GCA Cuts at: 0 3772 4110 Size: 3772 338 FspAI rTGC'GCAy Cuts at: 0 3772 4110 Size: 3772 338 HaeII r_GCGC'y Cuts at: 0 74 837 1434 4110 Size: 74 763 597 2676 Fragments arranged by size: 2676 763 597 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 621 653 1067 1099 1462 1494 1696 1728 Size: 621 32 414 32 363 32 202 32 Cuts at: 1728 1760 1870 1902 2040 2072 2133 2165 2800 Size: 32 110 32 138 32 61 32 635 Cuts at: 2800 2832 3014 3046 3607 3639 3943 3975 3985 Size: 32 182 32 561 32 304 32 10 Cuts at: 3985 4017 4077 4109 4110 Size: 32 60 32 1 Fragments arranged by size: 635 621 561 414 363 304 202 182 138 110 61 60 32 32 32 32 32 32 32 32 32 32 32 32 32 32 10 1 HincII GTy'rAC Cuts at: 0 3141 3825 4110 Size: 3141 684 285 Fragments arranged by size: 3141 684 285 KasI G'GCGC_C Cuts at: 0 70 1430 4110 Size: 70 1360 2680 Fragments arranged by size: 2680 1360 70 KpnI G_GTAC'C Cuts at: 0 566 4110 Size: 566 3544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 607 1645 1682 1724 1961 4110 Size: 607 1038 37 42 237 2149 Fragments arranged by size: 2149 1038 607 237 42 37 MscI TGG'CCA Cuts at: 0 4 466 4110 Size: 4 462 3644 Fragments arranged by size: 3644 462 4 MslI CAynn'nnrTG Cuts at: 0 1774 3772 4051 4110 Size: 1774 1998 279 59 Fragments arranged by size: 1998 1774 279 59 MspA1I CmG'CkG Cuts at: 0 39 76 1135 2925 4110 Size: 39 37 1059 1790 1185 Fragments arranged by size: 1790 1185 1059 39 37 NaeI GCC'GGC Cuts at: 0 16 2118 4110 Size: 16 2102 1992 Fragments arranged by size: 2102 1992 16 NarI GG'CG_CC Cuts at: 0 71 1431 4110 Size: 71 1360 2679 Fragments arranged by size: 2679 1360 71 NgoMIV G'CCGG_C Cuts at: 0 14 2116 4110 Size: 14 2102 1994 Fragments arranged by size: 2102 1994 14 NotI GC'GGCC_GC Cuts at: 0 29 4110 Size: 29 4081 NspI r_CATG'y Cuts at: 0 3621 3777 4110 Size: 3621 156 333 Fragments arranged by size: 3621 333 156 PasI CC'CwG_GG Cuts at: 0 1726 4110 Size: 1726 2384 PciI A'CATG_T Cuts at: 0 3617 4110 Size: 3617 493 PflMI CCAn_nnn'nTGG Cuts at: 0 1404 1664 1965 3219 4110 Size: 1404 260 301 1254 891 Fragments arranged by size: 1404 1254 891 301 260 PfoI T'CCnGG_A Cuts at: 0 488 824 3812 4110 Size: 488 336 2988 298 Fragments arranged by size: 2988 488 336 298 PmlI CAC'GTG Cuts at: 0 3219 4110 Size: 3219 891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 380 412 3853 3885 4110 Size: 380 32 3441 32 225 Fragments arranged by size: 3441 380 225 32 32 PpuMI rG'GwC_Cy Cuts at: 0 300 861 997 2048 2249 3438 4001 4110 Size: 300 561 136 1051 201 1189 563 109 Fragments arranged by size: 1189 1051 563 561 300 201 136 109 PshAI GACnn'nnGTC Cuts at: 0 554 1786 3743 4110 Size: 554 1232 1957 367 Fragments arranged by size: 1957 1232 554 367 PspXI vC'TCGA_Gb Cuts at: 0 2043 4110 Size: 2043 2067 PstI C_TGCA'G Cuts at: 0 2432 2698 4110 Size: 2432 266 1412 Fragments arranged by size: 2432 1412 266 PvuII CAG'CTG Cuts at: 0 1135 4110 Size: 1135 2975 SacI G_AGCT'C Cuts at: 0 455 1070 4110 Size: 455 615 3040 Fragments arranged by size: 3040 615 455 SacII CC_GC'GG Cuts at: 0 77 4110 Size: 77 4033 SanDI GG'GwC_CC Cuts at: 0 997 4110 Size: 997 3113 SapI GCTCTTCn'nnn_ Cuts at: 0 274 3292 4110 Size: 274 3018 818 Fragments arranged by size: 3018 818 274 SexAI A'CCwGG_T Cuts at: 0 1964 2050 4110 Size: 1964 86 2060 Fragments arranged by size: 2060 1964 86 SfcI C'TryA_G Cuts at: 0 2428 2694 2724 4110 Size: 2428 266 30 1386 Fragments arranged by size: 2428 1386 266 30 SfoI GGC'GCC Cuts at: 0 72 1432 4110 Size: 72 1360 2678 Fragments arranged by size: 2678 1360 72 SmaI CCC'GGG Cuts at: 0 421 4110 Size: 421 3689 SmlI C'TyrA_G Cuts at: 0 286 382 1747 2043 3020 3832 4110 Size: 286 96 1365 296 977 812 278 Fragments arranged by size: 1365 977 812 296 286 278 96 SspI AAT'ATT Cuts at: 0 2823 4110 Size: 2823 1287 StuI AGG'CCT Cuts at: 0 695 2352 3187 3680 4110 Size: 695 1657 835 493 430 Fragments arranged by size: 1657 835 695 493 430 StyI C'CwwG_G Cuts at: 0 1007 1256 1286 1424 2253 2507 3245 3675 Size: 1007 249 30 138 829 254 738 430 Cuts at: 3675 4110 Size: 435 Fragments arranged by size: 1007 829 738 435 430 254 249 138 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1739 1972 3180 4085 4110 Size: 1739 233 1208 905 25 Fragments arranged by size: 1739 1208 905 233 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 772 804 4110 Size: 772 32 3306 Fragments arranged by size: 3306 772 32 Tth111I GACn'n_nGTC Cuts at: 0 2052 4110 Size: 2052 2058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 753 4110 Size: 753 3357 XhoI C'TCGA_G Cuts at: 0 286 2043 3020 4110 Size: 286 1757 977 1090 Fragments arranged by size: 1757 1090 977 286 XmaI C'CCGG_G Cuts at: 0 419 4110 Size: 419 3691 XmnI GAAnn'nnTTC Cuts at: 0 1357 3202 4110 Size: 1357 1845 908 Fragments arranged by size: 1845 1357 908 ZraI GAC'GTC Cuts at: 0 1055 2811 4110 Size: 1055 1756 1299 Fragments arranged by size: 1756 1299 1055 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspXI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsrGI BssHII BstBI BstZ17I ClaI DraI DrdI FalI FseI HindIII HpaI MfeI MluI NcoI NdeI NheI NruI NsiI PacI PmeI PsiI PspOMI PsrI PvuI RsrII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TatI XbaI