################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MboII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 146 GCGG CCGC(-3/-1) AcsI 221 GAATTT [GA]^AATT[CT] AfaI 405 GTAC GT^AC AocI 199 CCTAAGG CC^T[ACGT]AGG ApoI 221 GAATTT [GA]^AATT[CT] AvaI 419 CTCGGG C^[CT]CG[GA]G BbvI 251 GCAGC GCAGC(8/12) BcgI 254 GCATTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 361 CTAG C^TAG BpmI 56 CTCCAG CTGGAG(16/14) BsiYI 136 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 136 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 260 GTCTC GTCTC(1/5) BsmFI 120 GGGAC GGGAC(10/14) BsoBI 419 CTCGGG C^[CT]CG[GA]G BspMI 73 GCAGGT ACCTGC(4/8) BsrI 79 CCAGT ACTGG(1/-1) BstXI 110 CCACAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 380 GGATCT [GA]^GATC[CT] Bsu36I 199 CCTAAGG CC^T[ACGT]AGG DraII 120 GGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 199 CCTAAGG CC^T[ACGT]AGG EcoO109I 120 GGGACCT [GA]G^G[ACGT]CC[CT] Fnu4HI 251 GCAGC GC^[ACGT]GC HaeIII 45 GGCC GG^CC HinfI 429 GACTC G^A[ACGT]TC ItaI 251 GCAGC GC^[ACGT]GC MaeI 361 CTAG C^TAG MaeIII 50 GTGAC ^GT[ACGT]AC MflI 380 GGATCT [GA]^GATC[CT] MnlI 55 CCTC CCTC(7/6) NlaIII 444 CATG CATG^ NlaIV 120 GGGACC GG[ACGT]^[ACGT]CC PleI 429 GACTC GAGTC(4/5) PpuMI 120 GGGACCT [GA]G^G[AT]CC[CT] PstI 71 CTGCAG CTGCA^G RsaI 405 GTAC GT^AC SspI 235 AATATT AAT^ATT TaqI 263 TCGA T^CGA TseI 251 GCAGC G^C[AT]GC Tsp45I 50 GTGAC ^GT[GC]AC TthHB8I 263 TCGA T^CGA XhoII 380 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 340 GATCC GGATC(4/5) 380 GGATC AvaII 76 GGTCC G^G[AT]CC 121 GGACC BstF5I 229 CATCC GGATG(2/0) 87 GGATG Cac8I 105 GCAAGC GC[ACGT]^[ACGT]GC 288 GCCTGC FokI 229 CATCC GGATG(9/13) 87 GGATG MseI 239 TTAA T^TAA 295 TTAA MslI 111 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 329 CACTGCTATG SfcI 71 CTGCAG C^T[GA][CT]AG 176 CTACAG Tru9I 239 TTAA T^TAA 295 TTAA TspRI 280 CAGTG CA[GC]TG[ACGT][ACGT]^ 329 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### Cfr13I DdeI DpnII MboI NdeII Sau3AI Sau96I Tsp509I