################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcyI 346 GACGCC G[GA]^CG[CT]C AfaI 223 GTAC GT^AC AflIII 898 ACGCGT A^C[GA][CT]GT AlwNI 35 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 474 GGGCCC GGGCC^C Asp700I 775 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 843 CGAGGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 467 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1021 CACGTA [CT]AC^GT[GA] BsaHI 346 GACGCC G[GA]^CG[CT]C BsgI 352 GTGCAG GTGCAG(16/14) BsiEI 424 CGGCCG CG[GA][CT]^CG BsiWI 222 CGTACG C^GTACG BspMI 710 ACCTGC ACCTGC(4/8) BsrDI 596 GCAATG GCAATG(2/0) EagI 424 CGGCCG C^GGCCG EclXI 424 CGGCCG C^GGCCG Eco52I 424 CGGCCG C^GGCCG EcoNI 99 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 974 CCTTGG C^C[AT][AT]GG HaeII 362 AGCGCC [GA]GCGC^[CT] Hin1I 346 GACGCC G[GA]^CG[CT]C MaeII 1022 ACGT A^CGT MaeIII 589 GTCAC ^GT[ACGT]AC MluI 898 ACGCGT A^CGCGT RsaI 223 GTAC GT^AC SspI 580 AATATT AAT^ATT StyI 974 CCTTGG C^C[AT][AT]GG TaqI 1157 TCGA T^CGA Tsp45I 589 GTCAC ^GT[GC]AC Tsp509I 1109 AATT ^AATT TthHB8I 1157 TCGA T^CGA XmnI 775 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaI 787 CTCGGG C^[CT]CG[GA]G 841 CCCGAG BalI 521 TGGCCA TGG^CCA 884 TGGCCA BamHI 108 GGATCC G^GATCC 1011 GGATCC BlpI 550 GCTAAGC GC^T[ACGT]AGC 1056 GCTGAGC Bpu1102I 550 GCTAAGC GC^T[ACGT]AGC 1056 GCTGAGC BsmBI 895 GAGACG CGTCTC(1/5) 994 GAGACG BsoBI 787 CTCGGG C^[CT]CG[GA]G 841 CCCGAG BstAPI 191 GCAACGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 290 GCAACCAGTGC BstXI 85 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 497 CCAGAGCCCTGG BstYI 108 GGATCC [GA]^GATC[CT] 1011 GGATCC CelII 550 GCTAAGC GC^T[ACGT]AGC 1056 GCTGAGC HphI 747 GGTGA GGTGA(8/7) 590 TCACC MflI 108 GGATCC [GA]^GATC[CT] 1011 GGATCC MluNI 521 TGGCCA TGG^CCA 884 TGGCCA MscI 521 TGGCCA TGG^CCA 884 TGGCCA MslI 114 CACAGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 480 CACACCCATG NaeI 308 GCCGGC GCC^GGC 872 GCCGGC NgoMIV 308 GCCGGC G^CCGGC 872 GCCGGC PflMI 513 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 794 CCATCCAGTGG PshAI 817 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 897 GACGCGTGTC SacI 27 GAGCTC GAGCT^C 1159 GAGCTC SbfI 324 CCTGCAGG CCTGCA^GG 906 CCTGCAGG Sse8387I 324 CCTGCAGG CCTGCA^GG 906 CCTGCAGG Van91I 513 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 794 CCATCCAGTGG XhoII 108 GGATCC [GA]^GATC[CT] 1011 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StuI TseI TspRI