Restriction map for
pFN21AB7686
(Circular) (Six-base) MAPSORT of: pFN21AB7686.seq Check: 150 from: 1 to: 5935
pFN21AB7686 5935 bp
With 182 enzymes: SgfI *
March 28, 2012 12:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 3234 4062 4435 1563
Size: 1671 828 373 3063
Fragments arranged by size:
3063 1671 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3032 3998 5382 5650 1632
Size: 707 693 966 1384 268 1917
Fragments arranged by size:
1917 1384 966 707 693 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 2903 4834 5811 1203
Size: 1700 1931 977 1327
Fragments arranged by size:
1931 1700 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 2123 1166
Size: 957 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2287 1184
Size: 1103 4832
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 2772
Size: 32 5903
AlwNI CAG_nnn'CTG
Cuts at: 2045 3678 5250 2045
Size: 1633 1572 2730
Fragments arranged by size:
2730 1633 1572
ApaI G_GGCC'C
Cuts at: 2483 2483
Size: 5935
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 2846 3260 1870
Size: 73 849 54 414 4545
Fragments arranged by size:
4545 849 414 73 54
BamHI G'GATC_C
Cuts at: 2113 3016 3265 3632 2113
Size: 903 249 367 4416
Fragments arranged by size:
4416 903 367 249
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2839 2867 3256 4711
Size: 325 217 627 463 589 28 389 1455
Cuts at: 4711 618
Size: 1842
Fragments arranged by size:
1842 1455 627 589 463 389 325 217
28
BanII G_rGCy'C
Cuts at: 1391 2036 2132 2483 2509 3168 3254 1391
Size: 645 96 351 26 659 86 4072
Fragments arranged by size:
4072 659 645 351 96 86 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2837 2871 4126 4160 1903
Size: 34 900 34 1255 34 3678
Fragments arranged by size:
3678 1255 900 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2478 4546 136
Size: 107 122 71 631 605 806 2068 1525
Fragments arranged by size:
2068 1525 806 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2556 3062 3352 2556
Size: 506 290 5139
Fragments arranged by size:
5139 506 290
Bme1580I G_kGCm'C
Cuts at: 1790 2276 2483 2872 3996 5152 1790
Size: 486 207 389 1124 1156 2573
Fragments arranged by size:
2573 1156 1124 486 389 207
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2073 2123 2582 2713 4595 1239
Size: 820 14 50 459 131 1882 2579
Fragments arranged by size:
2579 1882 820 459 131 50 14
Bpu10I CC'TnA_GC
Cuts at: 3021 3021
Size: 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4060 4925 5223 4060
Size: 865 298 4772
Fragments arranged by size:
4772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2663 2814 4598 882
Size: 617 504 660 151 1784 2219
Fragments arranged by size:
2219 1784 660 617 504 151
BsaAI yAC'GTr
Cuts at: 493 1204 3028 3726 493
Size: 711 1824 698 2702
Fragments arranged by size:
2702 1824 711 698
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2352 3742 4124 4759
Size: 53 83 186 936 819 1390 382 635
Cuts at: 4759 275
Size: 1451
Fragments arranged by size:
1451 1390 936 819 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2772 2802 1178
Size: 30 165 30 1369 30 4311
Fragments arranged by size:
4311 1369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2236 2611 2623 2780 3116 3158 1186
Size: 1050 375 12 157 336 42 3963
Fragments arranged by size:
3963 1050 375 336 157 42 12
BseYI C'CCAG_C
Cuts at: 2063 3089 5138 5634 2063
Size: 1026 2049 496 2364
Fragments arranged by size:
2364 2049 1026 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4720
Fragments arranged by size:
4720 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2432 3385 4146 5174 1540
Size: 275 186 431 953 761 1028 2301
Fragments arranged by size:
2301 1028 953 761 431 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 3027 3168 3996 4081 5152 2036
Size: 991 141 828 85 1071 2819
Fragments arranged by size:
2819 1071 991 828 141 85
BsiWI C'GTAC_G
Cuts at: 2227 2227
Size: 5935
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2894 2993 2894
Size: 99 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2132 2276 2483 2509 2872 3027
Size: 399 246 96 144 207 26 363 155
Cuts at: 3027 3168 3254 3996 4081 5152 1391
Size: 141 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 246 207 155
144 141 96 86 85 26
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2724 3290 844
Size: 1880 566 3489
Fragments arranged by size:
3489 1880 566
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 4430 4604 65
Size: 1183 1360 1822 174 1396
Fragments arranged by size:
1822 1396 1360 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 2861 2877 4579 4766 1700
Size: 110 503 548 16 1702 187 2869
Fragments arranged by size:
2869 1702 548 503 187 110 16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2202 2301 794
Size: 1408 99 4428
Fragments arranged by size:
4428 1408 99
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 2097 2509 2097
Size: 412 5523
BstYI r'GATC_y
Cuts at: 1972 2113 3016 3265 3632 4017 4034 5486 5572
Size: 141 903 249 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 903 385 367 346 249 141
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 2474 2955 513
Size: 552 939 273 197 481 3493
Fragments arranged by size:
3493 939 552 481 273 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 3147 3451 4245 4265 3147
Size: 304 794 20 4817
Fragments arranged by size:
4817 794 304 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2944 2979 529
Size: 35 2380 35 3485
Fragments arranged by size:
3485 2380 35 35
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2429 2526 2694
Size: 54 1425 50 275 18 599 97 168
Cuts at: 2694 2889 3382 4271 5900 8
Size: 195 493 889 1629 43
Fragments arranged by size:
1629 1425 889 599 493 275 195 168
97 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2429 3382 1537
Size: 275 617 953 4090
Fragments arranged by size:
4090 953 617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2641 2693 2760 2993 3865 1353
Size: 1288 52 67 233 872 3423
Fragments arranged by size:
3423 1288 872 233 67 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2248 4522 4896 5042 387
Size: 1064 138 659 2274 374 146 1280
Fragments arranged by size:
2274 1280 1064 659 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 3166 2034
Size: 1132 4803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2073 2123 2339 2582 2713 3032
Size: 393 427 14 50 216 243 131 319
Cuts at: 3032 3998 4595 5382 5650 1239
Size: 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 597 427 393 319 268
243 216 131 50 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 2108
Size: 5935
EcoO109I rG'GnC_Cy
Cuts at: 2101 2479 2480 3007 2101
Size: 378 1 527 5029
Fragments arranged by size:
5029 527 378 1
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 2371 5082 1678
Size: 693 2711 2531
Fragments arranged by size:
2711 2531 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 4576 4608 4650
Size: 32 135 32 1090 32 1882 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 1882 1090 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 2903 5811 2903
Size: 2908 3027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 5049 5233 1305
Size: 1134 2610 184 2007
Fragments arranged by size:
2610 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 2891 10
Size: 54 1425 343 696 363 3054
Fragments arranged by size:
3054 1425 696 363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2489 3893 4252 4411 518
Size: 648 957 366 1404 359 159 2042
Fragments arranged by size:
2042 1404 957 648 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2063 2169 2208 2543 2826 3351 4028
Size: 445 12 106 39 335 283 525 677
Cuts at: 4028 5176 5421 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 445 335 283 245
106 39 12
NaeI GCC'GGC
Cuts at: 2315 2879 2315
Size: 564 5371
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NgoMIV G'CCGG_C
Cuts at: 2313 2877 2313
Size: 564 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2509 2872 1348
Size: 1161 363 4411
Fragments arranged by size:
4411 1161 363
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 2805 1108
Size: 1416 281 4238
Fragments arranged by size:
4238 1416 281
PfoI T'CCnGG_A
Cuts at: 2075 2742 2075
Size: 667 5268
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2772 2804 4004 4036 5517 5549 2772
Size: 32 1200 32 1481 32 3158
Fragments arranged by size:
3158 1481 1200 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2826 2906 1613
Size: 1213 80 4642
Fragments arranged by size:
4642 1213 80
PsiI TTA'TAA
Cuts at: 2537 3505 2537
Size: 968 4967
PspOMI G'GGCC_C
Cuts at: 2479 2479
Size: 5935
PstI C_TGCA'G
Cuts at: 830 2050 2062 2207 2334 2500 2916 3287 830
Size: 1220 12 145 127 166 416 371 3478
Fragments arranged by size:
3478 1220 416 371 166 145 127 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2051 2063 2543 2826 2051
Size: 12 480 283 5160
Fragments arranged by size:
5160 480 283 12
SacI G_AGCT'C
Cuts at: 2036 3168 2036
Size: 1132 4803
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 2334 2916 3287 2334
Size: 582 371 4982
Fragments arranged by size:
4982 582 371
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2046 2058 2203 2245 2330 2496 2912
Size: 219 1001 12 145 42 85 166 416
Cuts at: 2912 3283 4418 5099 5290 826
Size: 371 1135 681 191 1471
Fragments arranged by size:
1471 1135 1001 681 416 371 219 191
166 145 85 42 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4039 4940 5202 820
Size: 197 926 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 2587 3859 5
Size: 47 2535 1272 2081
Fragments arranged by size:
2535 2081 1272 47
StuI AGG'CCT
Cuts at: 1517 2309 2852 2978 3035 1517
Size: 792 543 126 57 4417
Fragments arranged by size:
4417 792 543 126 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2979 3374 513
Size: 552 939 975 395 3074
Fragments arranged by size:
3074 975 939 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 3962 3979 4132 4317 4762 325
Size: 1255 923 1459 17 153 185 445 1498
Fragments arranged by size:
1498 1459 1255 923 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4181 5893 96
Size: 274 80 33 51 1443 2204 1712 138
Fragments arranged by size:
2204 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 5895 5927 2756
Size: 32 3107 32 2764
Fragments arranged by size:
3107 2764 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 2784 4064 2784
Size: 1280 4655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI