(Linear) (Six-base) MAPSORT of: KIBB7686.seq Check: 96 from: 1 to: 1230 KIBB7686.orf With 182 enzymes: SgfI * March 22, 2012 15:39 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 1027 1230 Size: 334 693 203 Fragments arranged by size: 693 334 203 AflIII A'CryG_T Cuts at: 0 898 1230 Size: 898 332 AleI CACnn'nnGTG Cuts at: 0 118 1230 Size: 118 1112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 282 1230 Size: 282 948 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 767 799 1230 Size: 767 32 431 Fragments arranged by size: 767 431 32 AlwNI CAG_nnn'CTG Cuts at: 0 40 1230 Size: 40 1190 ApaI G_GGCC'C Cuts at: 0 478 1230 Size: 478 752 AvaI C'yCGr_G Cuts at: 0 787 841 1230 Size: 787 54 389 Fragments arranged by size: 787 389 54 BamHI G'GATC_C Cuts at: 0 108 1011 1230 Size: 108 903 219 Fragments arranged by size: 903 219 108 BanI G'GyrC_C Cuts at: 0 245 834 862 1230 Size: 245 589 28 368 Fragments arranged by size: 589 368 245 28 BanII G_rGCy'C Cuts at: 0 31 127 478 504 1163 1230 Size: 31 96 351 26 659 67 Fragments arranged by size: 659 351 96 67 31 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 832 866 1230 Size: 832 34 364 Fragments arranged by size: 832 364 34 BglI GCCn_nnn'nGGC Cuts at: 0 473 1230 Size: 473 757 BlpI GC'TnA_GC Cuts at: 0 551 1057 1230 Size: 551 506 173 Fragments arranged by size: 551 506 173 Bme1580I G_kGCm'C Cuts at: 0 271 478 867 1230 Size: 271 207 389 363 Fragments arranged by size: 389 363 271 207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 630 1230 Size: 630 600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 68 118 577 708 1230 Size: 54 14 50 459 131 522 Fragments arranged by size: 522 459 131 54 50 14 Bpu10I CC'TnA_GC Cuts at: 0 1016 1230 Size: 1016 214 BsaI GGTCTCn'nnnn_ Cuts at: 0 658 809 1230 Size: 658 151 421 Fragments arranged by size: 658 421 151 BsaAI yAC'GTr Cuts at: 0 1023 1230 Size: 1023 207 BsaHI Gr'CG_yC Cuts at: 0 347 1230 Size: 347 883 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 767 797 1230 Size: 767 30 433 Fragments arranged by size: 767 433 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 231 606 618 775 1111 1153 1230 Size: 231 375 12 157 336 42 77 Fragments arranged by size: 375 336 231 157 77 42 12 BseYI C'CCAG_C Cuts at: 0 58 1084 1230 Size: 58 1026 146 Fragments arranged by size: 1026 146 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 1230 Size: 373 857 BsiEI CG_ry'CG Cuts at: 0 427 1230 Size: 427 803 BsiHKAI G_wGCw'C Cuts at: 0 31 1022 1163 1230 Size: 31 991 141 67 Fragments arranged by size: 991 141 67 31 BsiWI C'GTAC_G Cuts at: 0 222 1230 Size: 222 1008 BsmBI CGTCTCn'nnnn_ Cuts at: 0 889 988 1230 Size: 889 99 242 Fragments arranged by size: 889 242 99 Bsp1286I G_dGCh'C Cuts at: 0 31 127 271 478 504 867 1022 1163 Size: 31 96 144 207 26 363 155 141 Cuts at: 1163 1230 Size: 67 Fragments arranged by size: 363 207 155 144 141 96 67 31 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 719 1230 Size: 719 511 BsrDI GCAATG_nn' Cuts at: 0 603 1230 Size: 603 627 BsrFI r'CCGG_y Cuts at: 0 308 856 872 1230 Size: 308 548 16 358 Fragments arranged by size: 548 358 308 16 BstAPI GCAn_nnn'nTGC Cuts at: 0 197 296 1230 Size: 197 99 934 Fragments arranged by size: 934 197 99 BstXI CCAn_nnnn'nTGG Cuts at: 0 92 504 1230 Size: 92 412 726 Fragments arranged by size: 726 412 92 BstYI r'GATC_y Cuts at: 0 108 1011 1230 Size: 108 903 219 Fragments arranged by size: 903 219 108 BtgI C'CryG_G Cuts at: 0 272 469 950 1230 Size: 272 197 481 280 Fragments arranged by size: 481 280 272 197 BtsI GCAGTG_nn' Cuts at: 0 1142 1230 Size: 1142 88 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 939 974 1230 Size: 939 35 256 Fragments arranged by size: 939 256 35 EaeI y'GGCC_r Cuts at: 0 424 521 689 884 1230 Size: 424 97 168 195 346 Fragments arranged by size: 424 346 195 168 97 EagI C'GGCC_G Cuts at: 0 424 1230 Size: 424 806 EarI CTCTTCn'nnn_ Cuts at: 0 636 688 755 988 1230 Size: 636 52 67 233 242 Fragments arranged by size: 636 242 233 67 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 243 1230 Size: 243 987 EcoICRI GAG'CTC Cuts at: 0 29 1161 1230 Size: 29 1132 69 Fragments arranged by size: 1132 69 29 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 68 118 334 577 708 1027 1230 Size: 54 14 50 216 243 131 319 203 Fragments arranged by size: 319 243 216 203 131 54 50 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 103 1230 Size: 103 1127 EcoO109I rG'GnC_Cy Cuts at: 0 96 474 475 1002 1230 Size: 96 378 1 527 228 Fragments arranged by size: 527 378 228 96 1 HaeII r_GCGC'y Cuts at: 0 366 1230 Size: 366 864 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 657 689 1230 Size: 657 32 541 Fragments arranged by size: 657 541 32 MluI A'CGCG_T Cuts at: 0 898 1230 Size: 898 332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 434 1230 Size: 434 796 MscI TGG'CCA Cuts at: 0 523 886 1230 Size: 523 363 344 Fragments arranged by size: 523 363 344 MslI CAynn'nnrTG Cuts at: 0 118 484 1230 Size: 118 366 746 Fragments arranged by size: 746 366 118 MspA1I CmG'CkG Cuts at: 0 46 58 164 203 538 821 1230 Size: 46 12 106 39 335 283 409 Fragments arranged by size: 409 335 283 106 46 39 12 NaeI GCC'GGC Cuts at: 0 310 874 1230 Size: 310 564 356 Fragments arranged by size: 564 356 310 NgoMIV G'CCGG_C Cuts at: 0 308 872 1230 Size: 308 564 358 Fragments arranged by size: 564 358 308 PasI CC'CwG_GG Cuts at: 0 504 867 1230 Size: 504 363 363 Fragments arranged by size: 504 363 363 PflMI CCAn_nnn'nTGG Cuts at: 0 519 800 1230 Size: 519 281 430 Fragments arranged by size: 519 430 281 PfoI T'CCnGG_A Cuts at: 0 70 737 1230 Size: 70 667 493 Fragments arranged by size: 667 493 70 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 767 799 1230 Size: 767 32 431 Fragments arranged by size: 767 431 32 PshAI GACnn'nnGTC Cuts at: 0 821 901 1230 Size: 821 80 329 Fragments arranged by size: 821 329 80 PsiI TTA'TAA Cuts at: 0 532 1230 Size: 532 698 PspOMI G'GGCC_C Cuts at: 0 474 1230 Size: 474 756 PstI C_TGCA'G Cuts at: 0 45 57 202 329 495 911 1230 Size: 45 12 145 127 166 416 319 Fragments arranged by size: 416 319 166 145 127 45 12 PvuII CAG'CTG Cuts at: 0 46 58 538 821 1230 Size: 46 12 480 283 409 Fragments arranged by size: 480 409 283 46 12 SacI G_AGCT'C Cuts at: 0 31 1163 1230 Size: 31 1132 67 Fragments arranged by size: 1132 67 31 SbfI CC_TGCA'GG Cuts at: 0 329 911 1230 Size: 329 582 319 Fragments arranged by size: 582 329 319 SfcI C'TryA_G Cuts at: 0 41 53 198 240 325 491 907 1230 Size: 41 12 145 42 85 166 416 323 Fragments arranged by size: 416 323 166 145 85 42 41 12 SspI AAT'ATT Cuts at: 0 582 1230 Size: 582 648 StuI AGG'CCT Cuts at: 0 304 847 973 1030 1230 Size: 304 543 126 57 200 Fragments arranged by size: 543 304 200 126 57 StyI C'CwwG_G Cuts at: 0 974 1230 Size: 974 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 498 1230 Size: 498 732 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 751 783 1230 Size: 751 32 447 Fragments arranged by size: 751 447 32 XmnI GAAnn'nnTTC Cuts at: 0 779 1230 Size: 779 451 Enzymes that do cut: AcuI AflIII AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BcgI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MluI MmeI MscI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI PpiI PshAI PsiI PspOMI PstI PvuII SacI SbfI SfcI SspI StuI StyI TaqII TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpuMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI