################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I EcoO65I EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 100 GAATTC [GA]^AATT[CT] AcyI 2114 GGCGCC G[GA]^CG[CT]C AflIII 381 ACATGT A^C[GA][CT]GT Alw44I 243 GTGCAC G^TGCAC Aor51HI 365 AGCGCT AGC^GCT ApaLI 243 GTGCAC G^TGCAC ApoI 100 GAATTC [GA]^AATT[CT] AspI 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2114 GGCGCC GGCGC^C BcgI 1646 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 2017 CTAG C^TAG BglI 999 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2570 AGATCT A^GATCT BlpI 2880 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2880 GCTCAGC GC^T[ACGT]AGC BsaHI 2114 GGCGCC G[GA]^CG[CT]C BsmBI 949 CGTCTC CGTCTC(1/5) BsmI 138 GAATGC GAATGC(1/-1) BspLU11I 381 ACATGT A^CATGT BsrDI 1303 CATTGC GCAATG(2/0) BstBI 59 TTCGAA TT^CGAA CelII 2880 GCTCAGC GC^T[ACGT]AGC DraIII 1357 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 365 AGCGCT AGC^GCT EcoRI 100 GAATTC G^AATTC FseI 2584 GGCCGGCC GGCCGG^CC Hin1I 2114 GGCGCC G[GA]^CG[CT]C KasI 2114 GGCGCC G^GCGCC MaeI 2017 CTAG C^TAG NarI 2114 GGCGCC GG^CGCC NcoI 200 CCATGG C^CATGG NotI 2865 GCGGCCGC GC^GGCCGC NspV 59 TTCGAA TT^CGAA PflFI 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 2883 CAGCTG CAG^CTG SfoI 2114 GGCGCC GGC^GCC SfuI 59 TTCGAA TT^CGAA StuI 1520 AGGCCT AGG^CCT Tsp509I 101 AATT ^AATT Tth111I 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbsI 299 GAAGAC GAAGAC(2/6) 340 GAAGAC BbvCI 618 CCTCAGC CCTCAGC(-5/-2) 1884 GCTGAGG BpuAI 299 GAAGAC GAAGAC(2/6) 340 GAAGAC BsaI 196 GAGACC GGTCTC(1/5) 1117 GAGACC BsrFI 2585 GCCGGC [GA]^CCGG[CT] 2874 GCCGGC BstYI 332 AGATCC [GA]^GATC[CT] 2570 AGATCT Cfr10I 2585 GCCGGC [GA]^CCGG[CT] 2874 GCCGGC HgaI 948 GCGTC GACGC(5/10) 1269 GCGTC KspI 1015 CCGCGG CCGC^GG 2104 CCGCGG MaeII 638 ACGT A^CGT 1748 ACGT MflI 332 AGATCC [GA]^GATC[CT] 2570 AGATCT NaeI 2585 GCCGGC GCC^GGC 2874 GCCGGC NgoMIV 2585 GCCGGC G^CCGGC 2874 GCCGGC NspI 381 ACATGT [GA]CATG^[CT] 2357 ACATGC PaeR7I 1330 CTCGAG C^TCGAG 1524 CTCGAG PshAI 378 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1872 GACTCAGGTC PstI 1278 CTGCAG CTGCA^G 1823 CTGCAG SacII 1015 CCGCGG CCGC^GG 2104 CCGCGG SexAI 722 ACCAGGT A^CC[AT]GGT 1358 ACCTGGT XhoI 1330 CTCGAG C^TCGAG 1524 CTCGAG XhoII 332 AGATCC [GA]^GATC[CT] 2570 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI