(Linear) (Six-base) MAPSORT of: KIEE3336.seq Check: 370 from: 1 to: 2922 KIEE3336.orf With 182 enzymes: SgfI * March 22, 2012 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 10 765 2774 2922 Size: 10 755 2009 148 Fragments arranged by size: 2009 755 148 10 AccI GT'mk_AC Cuts at: 0 728 1289 2540 2922 Size: 728 561 1251 382 Fragments arranged by size: 1251 728 561 382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 453 750 763 1952 2922 Size: 149 304 297 13 1189 970 Fragments arranged by size: 1189 970 304 297 149 13 AfeI AGC'GCT Cuts at: 0 367 2922 Size: 367 2555 AflIII A'CryG_T Cuts at: 0 381 2922 Size: 381 2541 AleI CACnn'nnGTG Cuts at: 0 1747 2922 Size: 1747 1175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2497 2722 2782 2922 Size: 2497 225 60 140 Fragments arranged by size: 2497 225 140 60 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1336 1368 2922 Size: 1336 32 1554 Fragments arranged by size: 1554 1336 32 AlwNI CAG_nnn'CTG Cuts at: 0 1733 1950 2622 2922 Size: 1733 217 672 300 Fragments arranged by size: 1733 672 300 217 ApaI G_GGCC'C Cuts at: 0 845 1976 2832 2900 2922 Size: 845 1131 856 68 22 Fragments arranged by size: 1131 856 845 68 22 ApaLI G'TGCA_C Cuts at: 0 243 2922 Size: 243 2679 ApoI r'AATT_y Cuts at: 0 100 2922 Size: 100 2822 AvaI C'yCGr_G Cuts at: 0 33 1330 1475 1524 1847 2821 2842 2922 Size: 33 1297 145 49 323 974 21 80 Fragments arranged by size: 1297 974 323 145 80 49 33 21 BanI G'GyrC_C Cuts at: 0 43 53 427 1362 1602 1612 2114 2916 Size: 43 10 374 935 240 10 502 802 Cuts at: 2916 2922 Size: 6 Fragments arranged by size: 935 802 502 374 240 43 10 10 6 BanII G_rGCy'C Cuts at: 0 91 501 845 1001 1349 1567 1582 1976 Size: 91 410 344 156 348 218 15 394 Cuts at: 1976 2832 2900 2922 Size: 856 68 22 Fragments arranged by size: 856 410 394 348 344 218 156 91 68 22 15 BbeI G_GCGC'C Cuts at: 0 2118 2922 Size: 2118 804 BbsI GAAGACnn'nnnn_ Cuts at: 0 306 347 2922 Size: 306 41 2575 Fragments arranged by size: 2575 306 41 BbvCI CC'TCA_GC Cuts at: 0 619 1885 2922 Size: 619 1266 1037 Fragments arranged by size: 1266 1037 619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1635 1669 2922 Size: 1635 34 1253 Fragments arranged by size: 1635 1253 34 BglI GCCn_nnn'nGGC Cuts at: 0 1005 2922 Size: 1005 1917 BglII A'GATC_T Cuts at: 0 2570 2922 Size: 2570 352 BlpI GC'TnA_GC Cuts at: 0 2881 2922 Size: 2881 41 Bme1580I G_kGCm'C Cuts at: 0 247 845 1976 2832 2900 2921 2922 Size: 247 598 1131 856 68 21 1 Fragments arranged by size: 1131 856 598 247 68 21 1 BmgBI CAC'GTC Cuts at: 0 639 1749 2922 Size: 639 1110 1173 Fragments arranged by size: 1173 1110 639 BmrI ACTGGGnnnn_n' Cuts at: 0 901 1219 1411 1743 2604 2922 Size: 901 318 192 332 861 318 Fragments arranged by size: 901 861 332 318 318 192 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 588 1488 1581 2052 2922 Size: 588 900 93 471 870 Fragments arranged by size: 900 870 588 471 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 502 960 1077 1363 1483 2018 2677 Size: 244 258 458 117 286 120 535 659 Cuts at: 2677 2922 Size: 245 Fragments arranged by size: 659 535 458 286 258 245 244 120 117 Bpu10I CC'TnA_GC Cuts at: 0 619 1885 2623 2922 Size: 619 1266 738 299 Fragments arranged by size: 1266 738 619 299 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 315 1065 2922 Size: 315 750 1857 Fragments arranged by size: 1857 750 315 BsaI GGTCTCn'nnnn_ Cuts at: 0 190 1111 2922 Size: 190 921 1811 Fragments arranged by size: 1811 921 190 BsaHI Gr'CG_yC Cuts at: 0 2115 2922 Size: 2115 807 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 371 401 1336 1366 1991 2021 2922 Size: 371 30 935 30 625 30 901 Fragments arranged by size: 935 901 625 371 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 133 663 1009 1023 1411 1414 2922 Size: 133 530 346 14 388 3 1508 Fragments arranged by size: 1508 530 388 346 133 14 3 BseYI C'CCAG_C Cuts at: 0 312 1061 1129 2045 2390 2550 2885 2922 Size: 312 749 68 916 345 160 335 37 Fragments arranged by size: 916 749 345 335 312 160 68 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 2309 2713 2806 2922 Size: 38 2271 404 93 116 Fragments arranged by size: 2271 404 116 93 38 BsiEI CG_ry'CG Cuts at: 0 2284 2353 2869 2922 Size: 2284 69 516 53 Fragments arranged by size: 2284 516 69 53 BsiHKAI G_wGCw'C Cuts at: 0 91 247 1567 1582 2922 Size: 91 156 1320 15 1340 Fragments arranged by size: 1340 1320 156 91 15 BsmI GAATG_Cn' Cuts at: 0 144 2922 Size: 144 2778 BsmBI CGTCTCn'nnnn_ Cuts at: 0 955 2922 Size: 955 1967 Bsp1286I G_dGCh'C Cuts at: 0 91 247 501 845 1001 1349 1567 1582 Size: 91 156 254 344 156 348 218 15 Cuts at: 1582 1976 2832 2900 2921 2922 Size: 394 856 68 21 1 Fragments arranged by size: 856 394 348 344 254 218 156 156 91 68 21 15 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 10 765 2774 2922 Size: 10 755 2009 148 Fragments arranged by size: 2009 755 148 10 BsrBI CCG'CTC Cuts at: 0 2672 2826 2865 2922 Size: 2672 154 39 57 Fragments arranged by size: 2672 154 57 39 BsrDI GCAATG_nn' Cuts at: 0 1302 2922 Size: 1302 1620 BsrFI r'CCGG_y Cuts at: 0 2585 2874 2922 Size: 2585 289 48 Fragments arranged by size: 2585 289 48 BssSI C'ACGA_G Cuts at: 0 181 494 2860 2922 Size: 181 313 2366 62 Fragments arranged by size: 2366 313 181 62 BstBI TT'CG_AA Cuts at: 0 60 2922 Size: 60 2862 BstXI CCAn_nnnn'nTGG Cuts at: 0 984 1749 2205 2922 Size: 984 765 456 717 Fragments arranged by size: 984 765 717 456 BstYI r'GATC_y Cuts at: 0 332 2570 2922 Size: 332 2238 352 Fragments arranged by size: 2238 352 332 Bsu36I CC'TnA_GG Cuts at: 0 846 1148 1517 2037 2922 Size: 846 302 369 520 885 Fragments arranged by size: 885 846 520 369 302 BtgI C'CryG_G Cuts at: 0 200 1015 1607 1700 2104 2922 Size: 200 815 592 93 404 818 Fragments arranged by size: 818 815 592 404 200 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1421 2922 Size: 1421 1501 BtsI GCAGTG_nn' Cuts at: 0 1336 2085 2297 2922 Size: 1336 749 212 625 Fragments arranged by size: 1336 749 625 212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2061 2096 2922 Size: 2061 35 826 Fragments arranged by size: 2061 826 35 DraIII CAC_nnn'GTG Cuts at: 0 1362 2922 Size: 1362 1560 DrdI GACnn_nn'nnGTC Cuts at: 0 907 2275 2536 2922 Size: 907 1368 261 386 Fragments arranged by size: 1368 907 386 261 EaeI y'GGCC_r Cuts at: 0 2 647 665 935 1223 2243 2281 2350 Size: 2 645 18 270 288 1020 38 69 Cuts at: 2350 2648 2866 2922 Size: 298 218 56 Fragments arranged by size: 1020 645 298 288 270 218 69 56 38 18 2 EagI C'GGCC_G Cuts at: 0 2281 2350 2866 2922 Size: 2281 69 516 56 Fragments arranged by size: 2281 516 69 56 EarI CTCTTCn'nnn_ Cuts at: 0 1201 1462 1723 2922 Size: 1201 261 261 1199 Fragments arranged by size: 1201 1199 261 261 EcoICRI GAG'CTC Cuts at: 0 89 1565 1580 2922 Size: 89 1476 15 1342 Fragments arranged by size: 1476 1342 89 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 244 453 502 750 763 960 1077 Size: 149 95 209 49 248 13 197 117 Cuts at: 1077 1363 1483 1952 2018 2677 2922 Size: 286 120 469 66 659 245 Fragments arranged by size: 659 469 286 248 245 209 197 149 120 117 95 66 49 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 1768 2482 2626 2710 2922 Size: 1768 714 144 84 212 Fragments arranged by size: 1768 714 212 144 84 EcoO109I rG'GnC_Cy Cuts at: 0 435 804 841 842 1947 1973 2234 2897 Size: 435 369 37 1 1105 26 261 663 Cuts at: 2897 2922 Size: 25 Fragments arranged by size: 1105 663 435 369 261 37 26 25 1 EcoRI G'AATT_C Cuts at: 0 100 2922 Size: 100 2822 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2481 2736 2922 Size: 2481 255 186 Fragments arranged by size: 2481 255 186 FseI GG_CCGG'CC Cuts at: 0 2589 2922 Size: 2589 333 FspI TGC'GCA Cuts at: 0 1308 2473 2710 2770 2922 Size: 1308 1165 237 60 152 Fragments arranged by size: 1308 1165 237 152 60 HaeII r_GCGC'y Cuts at: 0 369 1539 1811 2118 2881 2922 Size: 369 1170 272 307 763 41 Fragments arranged by size: 1170 763 369 307 272 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1399 1431 2922 Size: 1399 32 1491 Fragments arranged by size: 1491 1399 32 KasI G'GCGC_C Cuts at: 0 2114 2922 Size: 2114 808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 965 1208 2450 2828 2922 Size: 965 243 1242 378 94 Fragments arranged by size: 1242 965 378 243 94 MscI TGG'CCA Cuts at: 0 4 667 937 1225 2922 Size: 4 663 270 288 1697 Fragments arranged by size: 1697 663 288 270 4 MslI CAynn'nnrTG Cuts at: 0 154 1747 2782 2922 Size: 154 1593 1035 140 Fragments arranged by size: 1593 1035 154 140 MspA1I CmG'CkG Cuts at: 0 1017 2045 2106 2370 2448 2871 2885 2922 Size: 1017 1028 61 264 78 423 14 37 Fragments arranged by size: 1028 1017 423 264 78 61 37 14 NaeI GCC'GGC Cuts at: 0 2587 2876 2922 Size: 2587 289 46 Fragments arranged by size: 2587 289 46 NarI GG'CG_CC Cuts at: 0 2115 2922 Size: 2115 807 NcoI C'CATG_G Cuts at: 0 200 2922 Size: 200 2722 NgoMIV G'CCGG_C Cuts at: 0 2585 2874 2922 Size: 2585 289 48 Fragments arranged by size: 2585 289 48 NotI GC'GGCC_GC Cuts at: 0 2866 2922 Size: 2866 56 NspI r_CATG'y Cuts at: 0 385 2361 2922 Size: 385 1976 561 Fragments arranged by size: 1976 561 385 PasI CC'CwG_GG Cuts at: 0 318 516 2198 2922 Size: 318 198 1682 724 Fragments arranged by size: 1682 724 318 198 PciI A'CATG_T Cuts at: 0 381 2922 Size: 381 2541 PflMI CCAn_nnn'nTGG Cuts at: 0 1637 1733 1950 2922 Size: 1637 96 217 972 Fragments arranged by size: 1637 972 217 96 PfoI T'CCnGG_A Cuts at: 0 260 416 1460 2922 Size: 260 156 1044 1462 Fragments arranged by size: 1462 1044 260 156 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1336 1368 2922 Size: 1336 32 1554 Fragments arranged by size: 1554 1336 32 PpuMI rG'GwC_Cy Cuts at: 0 435 1947 2234 2922 Size: 435 1512 287 688 Fragments arranged by size: 1512 688 435 287 PshAI GACnn'nnGTC Cuts at: 0 382 1876 2922 Size: 382 1494 1046 Fragments arranged by size: 1494 1046 382 PspOMI G'GGCC_C Cuts at: 0 841 1972 2828 2896 2922 Size: 841 1131 856 68 26 Fragments arranged by size: 1131 856 841 68 26 PspXI vC'TCGA_Gb Cuts at: 0 1330 1524 2922 Size: 1330 194 1398 Fragments arranged by size: 1398 1330 194 PstI C_TGCA'G Cuts at: 0 1282 1827 2922 Size: 1282 545 1095 Fragments arranged by size: 1282 1095 545 PvuII CAG'CTG Cuts at: 0 2885 2922 Size: 2885 37 SacI G_AGCT'C Cuts at: 0 91 1567 1582 2922 Size: 91 1476 15 1340 Fragments arranged by size: 1476 1340 91 15 SacII CC_GC'GG Cuts at: 0 1018 2107 2922 Size: 1018 1089 815 Fragments arranged by size: 1089 1018 815 SanDI GG'GwC_CC Cuts at: 0 435 2922 Size: 435 2487 SexAI A'CCwGG_T Cuts at: 0 722 1358 2922 Size: 722 636 1564 Fragments arranged by size: 1564 722 636 SfcI C'TryA_G Cuts at: 0 597 729 831 1278 1290 1823 2541 2922 Size: 597 132 102 447 12 533 718 381 Fragments arranged by size: 718 597 533 447 381 132 102 12 SfoI GGC'GCC Cuts at: 0 2116 2922 Size: 2116 806 SmaI CCC'GGG Cuts at: 0 35 1477 1849 2922 Size: 35 1442 372 1073 Fragments arranged by size: 1442 1073 372 35 SmlI C'TyrA_G Cuts at: 0 294 1080 1330 1524 2922 Size: 294 786 250 194 1398 Fragments arranged by size: 1398 786 294 250 194 StuI AGG'CCT Cuts at: 0 1522 2922 Size: 1522 1400 StyI C'CwwG_G Cuts at: 0 5 200 2273 2922 Size: 5 195 2073 649 Fragments arranged by size: 2073 649 195 5 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 910 1075 1459 1600 2922 Size: 910 165 384 141 1322 Fragments arranged by size: 1322 910 384 165 141 TatI w'GTAC_w Cuts at: 0 1667 2922 Size: 1667 1255 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 371 403 1991 2023 2922 Size: 371 32 1588 32 899 Fragments arranged by size: 1588 899 371 32 32 Tth111I GACn'n_nGTC Cuts at: 0 637 2922 Size: 637 2285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 120 180 207 1356 1749 2922 Size: 120 60 27 1149 393 1173 Fragments arranged by size: 1173 1149 393 120 60 27 XhoI C'TCGA_G Cuts at: 0 1330 1524 2922 Size: 1330 194 1398 Fragments arranged by size: 1398 1330 194 XmaI C'CCGG_G Cuts at: 0 33 1475 1847 2922 Size: 33 1442 372 1075 Fragments arranged by size: 1442 1075 372 33 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmtI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrGI BssHII BstAPI BstEII BstZ17I ClaI DraI EciI EcoRV FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NruI NsiI PacI PmeI PmlI PsiI PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI ZraI