(Circular) (Six-base) MAPSORT of: pFN21AE3201.seq Check: 2415 from: 1 to: 6118 pFN21AE3201 6118 bp With 182 enzymes: SgfI * March 28, 2012 12:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2535 3928 278 Size: 53 83 186 936 999 1393 2468 Fragments arranged by size: 2468 1393 999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2538 4181 5565 5833 1632 Size: 906 1643 1384 268 1917 Fragments arranged by size: 1917 1643 1384 906 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 2715 5017 5994 1203 Size: 1512 2302 977 1327 Fragments arranged by size: 2302 1512 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 2572 3861 5433 2572 Size: 1289 1572 3257 Fragments arranged by size: 3257 1572 1289 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2445 2805 3056 3427 3653 3805 5914 1493 Size: 952 360 251 371 226 152 2109 1697 Fragments arranged by size: 2109 1697 952 371 360 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2370 3443 1870 Size: 73 427 1073 4545 Fragments arranged by size: 4545 1073 427 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3439 4894 618 Size: 325 217 627 242 1410 1455 1842 Fragments arranged by size: 1842 1455 1410 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 2331 3437 1391 Size: 940 1106 4072 Fragments arranged by size: 4072 1106 940 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 6118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2173 2385 3309 928 Size: 971 235 39 212 924 3737 Fragments arranged by size: 3737 971 924 235 212 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2525 2559 4309 4343 1903 Size: 34 588 34 1750 34 3678 Fragments arranged by size: 3678 1750 588 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3119 4010 5220 3119 Size: 891 1210 4017 Fragments arranged by size: 4017 1210 891 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4729 136 Size: 107 122 71 631 605 337 2720 1525 Fragments arranged by size: 2720 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3174 6113 3174 Size: 2939 3179 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 4179 5335 1790 Size: 2389 1156 2573 Fragments arranged by size: 2573 2389 1156 BmgBI CAC'GTC Cuts at: 1318 3022 1318 Size: 1704 4414 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2856 3849 4807 Size: 416 53 83 399 24 1439 993 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1753 1439 993 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3063 4778 1239 Size: 1824 1715 2579 Fragments arranged by size: 2579 1824 1715 Bpu10I CC'TnA_GC Cuts at: 3012 3012 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 4243 5108 5406 2297 Size: 1946 865 298 3009 Fragments arranged by size: 3009 1946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 2793 4781 882 Size: 617 513 781 1988 2219 Fragments arranged by size: 2219 1988 781 617 513 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2532 3397 3925 Size: 53 83 186 936 497 502 865 528 Cuts at: 3925 4307 4942 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 865 635 528 502 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 1178 Size: 30 165 30 915 30 4948 Fragments arranged by size: 4948 915 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 3296 3299 3302 3311 3371 3382 1186 Size: 874 1236 3 3 9 60 11 3922 Fragments arranged by size: 3922 1236 874 60 11 9 3 3 BseYI C'CCAG_C Cuts at: 5321 5817 5321 Size: 496 5622 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5545 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2371 4179 4264 5335 2371 Size: 1808 85 1071 3154 Fragments arranged by size: 3154 1808 1071 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 BsmBI CGTCTCn'nnnn_ Cuts at: 2018 3355 2018 Size: 1337 4781 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2371 3437 4179 4264 5335 1391 Size: 399 541 40 1066 742 85 1071 2174 Fragments arranged by size: 2174 1071 1066 742 541 399 85 40 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 2149 4007 5737 2149 Size: 1858 1730 2530 Fragments arranged by size: 2530 1858 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 4613 4787 65 Size: 1183 922 2443 174 1396 Fragments arranged by size: 2443 1396 1183 922 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2899 96 Size: 2803 3315 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 2098 3174 3448 3815 4200 4217 5669 5755 Size: 126 1076 274 367 385 17 1452 86 Cuts at: 5755 5767 6113 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1076 385 367 346 274 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4618 Fragments arranged by size: 4618 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2637 3634 4428 4448 2637 Size: 997 794 20 4307 Fragments arranged by size: 4307 997 794 20 ClaI AT'CG_AT Cuts at: 1554 2431 1554 Size: 877 5241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3166 3422 3166 Size: 256 5862 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2044 5125 809 Size: 507 728 3081 1802 Fragments arranged by size: 3081 1802 728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3565 4454 Size: 54 1425 50 275 18 180 1555 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1555 1425 889 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2196 2243 3261 3273 3288 3348 4048 1353 Size: 843 47 1018 12 15 60 700 3423 Fragments arranged by size: 3423 1018 843 700 60 47 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2538 3063 4181 4778 5565 5833 1239 Size: 393 906 525 1118 597 787 268 1524 Fragments arranged by size: 1524 1118 906 787 597 525 393 268 EcoO109I rG'GnC_Cy Cuts at: 3038 3251 3038 Size: 213 5905 EcoRI G'AATT_C Cuts at: 2445 3427 3805 2445 Size: 982 378 4758 Fragments arranged by size: 4758 982 378 EcoRV GAT'ATC Cuts at: 2193 2193 Size: 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2975 2975 Size: 6118 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2033 5265 1678 Size: 355 3232 2531 Fragments arranged by size: 3232 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 4759 4791 4833 Size: 32 135 32 665 32 2490 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 2490 665 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 2414 748 Size: 1666 4452 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KasI G'GCGC_C Cuts at: 2029 2029 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2207 2961 3239 5232 5416 1305 Size: 902 754 278 1993 184 2007 Fragments arranged by size: 2007 1993 902 754 278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 4076 4435 4594 518 Size: 648 1464 1446 359 159 2042 Fragments arranged by size: 2042 1464 1446 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2158 2543 2705 3402 3534 4211 5359 Size: 429 123 385 162 697 132 677 1148 Cuts at: 5359 5604 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 697 677 429 385 245 162 132 123 NarI GG'CG_CC Cuts at: 2030 2030 Size: 6118 NcoI C'CATG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4618 Fragments arranged by size: 4618 939 552 9 NdeI CA'TA_TG Cuts at: 387 3204 387 Size: 2817 3301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2719 3213 3476 5021 1132 Size: 97 1490 494 263 1545 2229 Fragments arranged by size: 2229 1545 1490 494 263 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 2715 5017 2715 Size: 2302 3816 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3038 3251 3038 Size: 213 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PstI C_TGCA'G Cuts at: 830 2644 3470 830 Size: 1814 826 3478 Fragments arranged by size: 3478 1814 826 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2543 2705 2543 Size: 162 5956 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2640 3231 3466 4601 5282 5473 826 Size: 219 1595 591 235 1135 681 191 1471 Fragments arranged by size: 1595 1471 1135 681 591 235 219 191 SfoI GGC'GCC Cuts at: 2031 2031 Size: 6118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2370 4222 5123 5385 820 Size: 197 926 369 58 1852 901 262 1553 Fragments arranged by size: 1852 1553 926 901 369 262 197 58 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 2321 4042 5 Size: 47 2269 1721 2081 Fragments arranged by size: 2269 2081 1721 47 StuI AGG'CCT Cuts at: 1517 2300 2972 1517 Size: 783 672 4663 Fragments arranged by size: 4663 783 672 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 3557 513 Size: 552 939 9 1544 3074 Fragments arranged by size: 3074 1544 939 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 4145 4162 4315 4500 4945 325 Size: 1255 456 2109 17 153 185 445 1498 Fragments arranged by size: 2109 1498 1255 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 2899 4364 Size: 274 80 33 51 1443 645 277 1465 Cuts at: 4364 6076 96 Size: 1712 138 Fragments arranged by size: 1712 1465 1443 645 277 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 6078 6110 2318 Size: 32 3728 32 2326 Fragments arranged by size: 3728 2326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 2370 1943 Size: 427 5691 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 3030 4247 3030 Size: 1217 4901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2533 3926 276 Size: 53 83 186 936 999 1393 2468 Fragments arranged by size: 2468 1393 999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI