################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI HphI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MseI MunI NaeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 575 TCCGGA T^CCGGA AlwI 473 GGATC GGATC(4/5) AlwNI 711 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 708 CCTCAGG CC^T[ACGT]AGG ApaI 219 GGGCCC GGGCC^C AspI 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 84 CTCGGG C^[CT]CG[GA]G BbeI 140 GGCGCC GGCGC^C BbvCI 315 CCTCAGC CCTCAGC(-5/-2) BcgI 827 GCATCATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 917 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 575 TCCGGA [AT]^CCGG[AT] BseAI 575 TCCGGA T^CCGGA BseRI 657 CTCCTC GAGGAG(10/8) BsgI 763 GTGCAG GTGCAG(16/14) BsoBI 84 CTCGGG C^[CT]CG[GA]G BspEI 575 TCCGGA T^CCGGA BsrBI 691 CCGCTC CCGCTC(-3/-3) BsrDI 297 CATTGC GCAATG(2/0) BstAPI 639 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 20 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 708 CCTCAGG CC^T[ACGT]AGG Eco81I 708 CCTCAGG CC^T[ACGT]AGG EcoNI 627 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 140 GGCGCC [GA]GCGC^[CT] KasI 140 GGCGCC G^GCGCC MroI 575 TCCGGA T^CCGGA MslI 402 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 381 CAGCTG C[AC]G^C[GT]G NarI 140 GGCGCC GG^CGCC NcoI 662 CCATGG C^CATGG NdeI 542 CATATG CA^TATG PflFI 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 381 CAGCTG CAG^CTG SfoI 140 GGCGCC GGC^GCC SgrAI 770 CGCCGGTG C[GA]^CCGG[CT]G TaqI 476 TCGA T^CGA TspRI 405 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 476 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 452 ACGTGT A^C[GA][CT]GT 803 ACACGT AviII 64 TGCGCA TGC^GCA 125 TGCGCA BalI 98 TGGCCA TGG^CCA 293 TGGCCA BanII 219 GGGCCC G[GA]GC[CT]^C 915 GAGCCC BpmI 481 CTGGAG CTGGAG(16/14) 717 CTGGAG BsiEI 179 CGGCCG CG[GA][CT]^CG 758 CGGCCG BspMI 122 ACCTGC ACCTGC(4/8) 170 ACCTGC BsrFI 617 ACCGGC [GA]^CCGG[CT] 771 GCCGGT Cfr10I 617 ACCGGC [GA]^CCGG[CT] 771 GCCGGT DpnII 352 GATC ^GATC 474 GATC DraII 389 GGGTCCC [GA]G^G[ACGT]CC[CT] 979 GGGACCC EagI 179 CGGCCG C^GGCCG 758 CGGCCG EclXI 179 CGGCCG C^GGCCG 758 CGGCCG Eco52I 179 CGGCCG C^GGCCG 758 CGGCCG EcoO109I 389 GGGTCCC [GA]G^G[ACGT]CC[CT] 979 GGGACCC FspI 64 TGCGCA TGC^GCA 125 TGCGCA HgaI 442 GACGC GACGC(5/10) 768 GACGC HinfI 961 GAATC G^A[ACGT]TC 970 GAATC MaeII 452 ACGT A^CGT 805 ACGT MboI 352 GATC ^GATC 474 GATC MluNI 98 TGGCCA TGG^CCA 293 TGGCCA MscI 98 TGGCCA TGG^CCA 293 TGGCCA NdeII 352 GATC ^GATC 474 GATC PpuMI 389 GGGTCCC [GA]G^G[AT]CC[CT] 979 GGGACCC PstI 205 CTGCAG CTGCA^G 241 CTGCAG Sau3AI 352 GATC ^GATC 474 GATC SfcI 205 CTGCAG C^T[GA][CT]AG 241 CTGCAG TfiI 961 GAATC G^A[AT]TC 970 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI Ksp632I MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI SapI Sau96I ScrFI SfaNI StyI TseI