################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AhdI 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 85 GTGCAC G^TGCAC AlwNI 588 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 85 GTGCAC G^TGCAC AspEI 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 85 GTGCAC G[AT]GC[AT]^C AvaII 21 GGACC G^G[AT]CC BcnI 8 CCCGG CC^[GC]GG BfaI 256 CTAG C^TAG BglI 502 GCCAGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 179 AGATCT A^GATCT BpmI 277 CTGGAG CTGGAG(16/14) BsaWI 389 ACCGGA [AT]^CCGG[AT] BsiHKAI 85 GTGCAC G[AT]GC[AT]^C BspDI 376 ATCGAT AT^CGAT BstAPI 587 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 47 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 179 AGATCT [GA]^GATC[CT] ClaI 376 ATCGAT AT^CGAT DdeI 534 CTTAG C^T[ACGT]AG Eam1105I 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 298 GATATC GAT^ATC FauI 306 GCGGG CCCGC(4/6) MaeI 256 CTAG C^TAG MboII 12 GAAGA GAAGA(8/7) MflI 179 AGATCT [GA]^GATC[CT] NciI 8 CCCGG CC^[GC]GG PleI 280 GAGTC GAGTC(4/5) SmlI 413 CTCAAG C^T[CT][GA]AG XcmI 353 CCATGGTGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 179 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 396 GGTACC G^GTACC 521 GGTACC AfaI 397 GTAC GT^AC 522 GTAC Asp718I 396 GGTACC G^GTACC 521 GGTACC BalI 320 TGGCCA TGG^CCA 500 TGGCCA BanI 396 GGTACC G^G[CT][GA]CC 521 GGTACC BanII 237 GGGCTC G[GA]GC[CT]^C 509 GGGCTC BbvI 248 GCTGC GCAGC(8/12) 593 GCTGC BsmFI 185 GGGAC GGGAC(10/14) 331 GTCCC BsrBI 210 CCGCTC CCGCTC(-3/-3) 202 GAGCGG BstF5I 132 CATCC GGATG(2/0) 252 CATCC Cfr13I 21 GGACC G^G[ACGT]CC 125 GGGCC EaeI 320 TGGCCA [CT]^GGCC[GA] 500 TGGCCA Fnu4HI 248 GCTGC GC^[ACGT]GC 593 GCTGC FokI 132 CATCC GGATG(9/13) 252 CATCC HapII 9 CCGG C^CGG 390 CCGG HpaII 9 CCGG C^CGG 390 CCGG ItaI 248 GCTGC GC^[ACGT]GC 593 GCTGC KpnI 396 GGTACC GGTAC^C 521 GGTACC MaeII 94 ACGT A^CGT 226 ACGT MluNI 320 TGGCCA TGG^CCA 500 TGGCCA MscI 320 TGGCCA TGG^CCA 500 TGGCCA MspI 9 CCGG C^CGG 390 CCGG RsaI 397 GTAC GT^AC 522 GTAC Sau96I 21 GGACC G^G[ACGT]CC 125 GGGCC TaqI 377 TCGA T^CGA 517 TCGA TfiI 3 GAATC G^A[AT]TC 553 GAATC TseI 248 GCTGC G^C[AT]GC 593 GCTGC Tsp45I 158 GTGAC ^GT[GC]AC 268 GTCAC TspRI 119 CACTG CA[GC]TG[ACGT][ACGT]^ 456 CAGTG TthHB8I 377 TCGA T^CGA 517 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I DpnII DsaI EcoRII EcoT14I HaeIII HinfI HphI MaeIII MboI MnlI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI StyI