(Linear) (Six-base) MAPSORT of: KIEE9451.seq Check: 4726 from: 1 to: 2463 KIEE9451.orf With 182 enzymes: SgfI * March 22, 2012 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 742 2260 2463 Size: 742 1518 203 Fragments arranged by size: 1518 742 203 AatII G_ACGT'C Cuts at: 0 1195 2463 Size: 1195 1268 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 921 1053 1138 1546 1896 1915 2463 Size: 921 132 85 408 350 19 548 Fragments arranged by size: 921 548 408 350 132 85 19 AfeI AGC'GCT Cuts at: 0 1477 2463 Size: 1477 986 AleI CACnn'nnGTG Cuts at: 0 1429 1666 1943 2463 Size: 1429 237 277 520 Fragments arranged by size: 1429 520 277 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1961 2463 Size: 1961 502 AlwNI CAG_nnn'CTG Cuts at: 0 422 2463 Size: 422 2041 ApaLI G'TGCA_C Cuts at: 0 1049 2463 Size: 1049 1414 ApoI r'AATT_y Cuts at: 0 771 1174 1533 2463 Size: 771 403 359 930 Fragments arranged by size: 930 771 403 359 AvaI C'yCGr_G Cuts at: 0 1538 2463 Size: 1538 925 BamHI G'GATC_C Cuts at: 0 216 2463 Size: 216 2247 BanII G_rGCy'C Cuts at: 0 871 1787 2463 Size: 871 916 676 Fragments arranged by size: 916 871 676 BbsI GAAGACnn'nnnn_ Cuts at: 0 518 1203 2395 2463 Size: 518 685 1192 68 Fragments arranged by size: 1192 685 518 68 BbvCI CC'TCA_GC Cuts at: 0 1260 2463 Size: 1260 1203 BciVI GTATCCnnnnn_n' Cuts at: 0 2120 2463 Size: 2120 343 BclI T'GATC_A Cuts at: 0 2093 2463 Size: 2093 370 BfrBI ATG'CAT Cuts at: 0 2019 2463 Size: 2019 444 BglI GCCn_nnn'nGGC Cuts at: 0 743 2463 Size: 743 1720 Bme1580I G_kGCm'C Cuts at: 0 62 124 688 1053 2463 Size: 62 62 564 365 1410 Fragments arranged by size: 1410 564 365 62 62 BmgBI CAC'GTC Cuts at: 0 516 2463 Size: 516 1947 BmrI ACTGGGnnnn_n' Cuts at: 0 366 2393 2400 2463 Size: 366 2027 7 63 Fragments arranged by size: 2027 366 63 7 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1212 2463 Size: 1212 1251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1237 1583 1627 1677 2079 2463 Size: 1237 346 44 50 402 384 Fragments arranged by size: 1237 402 384 346 50 44 Bpu10I CC'TnA_GC Cuts at: 0 428 1260 2463 Size: 428 832 1203 Fragments arranged by size: 1203 832 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 2403 2463 Size: 648 1755 60 Fragments arranged by size: 1755 648 60 BsaI GGTCTCn'nnnn_ Cuts at: 0 646 1414 2463 Size: 646 768 1049 Fragments arranged by size: 1049 768 646 BsaAI yAC'GTr Cuts at: 0 1166 2463 Size: 1166 1297 BsaBI GATnn'nnATC Cuts at: 0 609 1381 1686 2463 Size: 609 772 305 777 Fragments arranged by size: 777 772 609 305 BsaHI Gr'CG_yC Cuts at: 0 1192 1774 2463 Size: 1192 582 689 Fragments arranged by size: 1192 689 582 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 696 726 2463 Size: 696 30 1737 Fragments arranged by size: 1737 696 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 2463 Size: 10 2453 BseYI C'CCAG_C Cuts at: 0 50 123 263 2210 2241 2398 2463 Size: 50 73 140 1947 31 157 65 Fragments arranged by size: 1947 157 140 73 65 50 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 548 837 1804 1921 2017 2463 Size: 336 212 289 967 117 96 446 Fragments arranged by size: 967 446 336 289 212 117 96 BsiEI CG_ry'CG Cuts at: 0 1106 2463 Size: 1106 1357 BsiHKAI G_wGCw'C Cuts at: 0 1053 1663 1787 2463 Size: 1053 610 124 676 Fragments arranged by size: 1053 676 610 124 BsmI GAATG_Cn' Cuts at: 0 432 676 1333 2021 2463 Size: 432 244 657 688 442 Fragments arranged by size: 688 657 442 432 244 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1183 2463 Size: 1183 1280 Bsp1286I G_dGCh'C Cuts at: 0 62 124 688 871 1053 1663 1787 2463 Size: 62 62 564 183 182 610 124 676 Fragments arranged by size: 676 610 564 183 182 124 62 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 23 742 1038 2260 2463 Size: 23 719 296 1222 203 Fragments arranged by size: 1222 719 296 203 23 BsrDI GCAATG_nn' Cuts at: 0 201 765 887 1009 2463 Size: 201 564 122 122 1454 Fragments arranged by size: 1454 564 201 122 122 BstAPI GCAn_nnn'nTGC Cuts at: 0 422 1759 2463 Size: 422 1337 704 Fragments arranged by size: 1337 704 422 BstXI CCAn_nnnn'nTGG Cuts at: 0 150 271 864 928 1431 1770 2463 Size: 150 121 593 64 503 339 693 Fragments arranged by size: 693 593 503 339 150 121 64 BstYI r'GATC_y Cuts at: 0 216 531 822 995 1587 2423 2463 Size: 216 315 291 173 592 836 40 Fragments arranged by size: 836 592 315 291 216 173 40 BtgI C'CryG_G Cuts at: 0 452 2463 Size: 452 2011 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 243 1791 2463 Size: 243 1548 672 Fragments arranged by size: 1548 672 243 BtsI GCAGTG_nn' Cuts at: 0 624 1028 1338 2463 Size: 624 404 310 1125 Fragments arranged by size: 1125 624 404 310 DraI TTT'AAA Cuts at: 0 1469 2463 Size: 1469 994 EaeI y'GGCC_r Cuts at: 0 621 1202 2349 2463 Size: 621 581 1147 114 Fragments arranged by size: 1147 621 581 114 EarI CTCTTCn'nnn_ Cuts at: 0 28 342 961 1181 1331 2221 2463 Size: 28 314 619 220 150 890 242 Fragments arranged by size: 890 619 314 242 220 150 28 EcoICRI GAG'CTC Cuts at: 0 1785 2463 Size: 1785 678 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 921 1053 1138 1237 1546 1583 1627 1677 Size: 921 132 85 99 309 37 44 50 Cuts at: 1677 1896 1915 2079 2463 Size: 219 19 164 384 Fragments arranged by size: 921 384 309 219 164 132 99 85 50 44 37 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 2267 2463 Size: 2267 196 EcoO109I rG'GnC_Cy Cuts at: 0 360 2315 2463 Size: 360 1955 148 Fragments arranged by size: 1955 360 148 EcoRI G'AATT_C Cuts at: 0 1533 2463 Size: 1533 930 EcoRV GAT'ATC Cuts at: 0 396 2463 Size: 396 2067 HaeII r_GCGC'y Cuts at: 0 1479 2463 Size: 1479 984 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 319 351 1521 1553 1567 1599 1740 1772 Size: 319 32 1170 32 14 32 141 32 Cuts at: 1772 2463 Size: 691 Fragments arranged by size: 1170 691 319 141 32 32 32 32 14 HincII GTy'rAC Cuts at: 0 447 2463 Size: 447 2016 HindIII A'AGCT_T Cuts at: 0 1712 2463 Size: 1712 751 MfeI C'AATT_G Cuts at: 0 1397 2463 Size: 1397 1066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 462 555 1331 2463 Size: 462 93 776 1132 Fragments arranged by size: 1132 776 462 93 MscI TGG'CCA Cuts at: 0 623 1204 2463 Size: 623 581 1259 Fragments arranged by size: 1259 623 581 MslI CAynn'nnrTG Cuts at: 0 679 1429 1666 1768 1930 1943 2463 Size: 679 750 237 102 162 13 520 Fragments arranged by size: 750 679 520 237 162 102 13 MspA1I CmG'CkG Cuts at: 0 50 320 473 878 941 1739 2463 Size: 50 270 153 405 63 798 724 Fragments arranged by size: 798 724 405 270 153 63 50 NcoI C'CATG_G Cuts at: 0 452 2463 Size: 452 2011 NdeI CA'TA_TG Cuts at: 0 1035 2463 Size: 1035 1428 NsiI A_TGCA'T Cuts at: 0 2021 2463 Size: 2021 442 NspI r_CATG'y Cuts at: 0 556 704 2463 Size: 556 148 1759 Fragments arranged by size: 1759 556 148 PasI CC'CwG_GG Cuts at: 0 864 2463 Size: 864 1599 PflMI CCAn_nnn'nTGG Cuts at: 0 760 789 2463 Size: 760 29 1674 Fragments arranged by size: 1674 760 29 PfoI T'CCnGG_A Cuts at: 0 219 1214 1654 1706 2463 Size: 219 995 440 52 757 Fragments arranged by size: 995 757 440 219 52 PmlI CAC'GTG Cuts at: 0 1166 2463 Size: 1166 1297 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2035 2067 2463 Size: 2035 32 396 Fragments arranged by size: 2035 396 32 PpuMI rG'GwC_Cy Cuts at: 0 360 2463 Size: 360 2103 PstI C_TGCA'G Cuts at: 0 1738 2312 2463 Size: 1738 574 151 Fragments arranged by size: 1738 574 151 PvuII CAG'CTG Cuts at: 0 50 320 473 878 941 1739 2463 Size: 50 270 153 405 63 798 724 Fragments arranged by size: 798 724 405 270 153 63 50 SacI G_AGCT'C Cuts at: 0 1787 2463 Size: 1787 676 SanDI GG'GwC_CC Cuts at: 0 360 2463 Size: 360 2103 SapI GCTCTTCn'nnn_ Cuts at: 0 2221 2463 Size: 2221 242 SbfI CC_TGCA'GG Cuts at: 0 2312 2463 Size: 2312 151 SexAI A'CCwGG_T Cuts at: 0 2182 2463 Size: 2182 281 SfcI C'TryA_G Cuts at: 0 102 462 873 1734 2308 2463 Size: 102 360 411 861 574 155 Fragments arranged by size: 861 574 411 360 155 102 SmlI C'TyrA_G Cuts at: 0 627 1538 2418 2463 Size: 627 911 880 45 Fragments arranged by size: 911 880 627 45 SphI G_CATG'C Cuts at: 0 556 2463 Size: 556 1907 StuI AGG'CCT Cuts at: 0 2203 2463 Size: 2203 260 StyI C'CwwG_G Cuts at: 0 452 783 1181 1364 2463 Size: 452 331 398 183 1099 Fragments arranged by size: 1099 452 398 331 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2226 2463 Size: 2226 237 TatI w'GTAC_w Cuts at: 0 388 2463 Size: 388 2075 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 696 728 1007 1039 1072 1104 2463 Size: 696 32 279 32 33 32 1359 Fragments arranged by size: 1359 696 279 33 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 358 442 2463 Size: 358 84 2021 Fragments arranged by size: 2021 358 84 XbaI T'CTAG_A Cuts at: 0 493 2463 Size: 493 1970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1212 2463 Size: 1212 1251 XhoI C'TCGA_G Cuts at: 0 1538 2463 Size: 1538 925 XmnI GAAnn'nnTTC Cuts at: 0 1909 2463 Size: 1909 554 ZraI GAC'GTC Cuts at: 0 1193 2463 Size: 1193 1270 Enzymes that do cut: AarI AatII AcuI AfeI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BglII BlpI BmtI BsaWI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EciI FalI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XmaI