Restriction map for
pFN21AE3768
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BciVI BclI BfrI BglII
BlnI BsiWI Bsp1407I BspDI BspHI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MfeI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 2754 TCCGGA T^CCGGA
AhdI 664 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 539 GTGCAC G^TGCAC
AocI 477 CCTTAGG CC^T[ACGT]AGG
ApaLI 539 GTGCAC G^TGCAC
AspEI 664 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 23 TGGCCA TGG^CCA
BamHI 1489 GGATCC G^GATCC
BcgI 987 CGAGCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1332 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1332 GCTGAGC GC^T[ACGT]AGC
BsaBI 1290 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2754 TCCGGA [AT]^CCGG[AT]
BseAI 2754 TCCGGA T^CCGGA
BsmBI 826 CGTCTC CGTCTC(1/5)
BsmI 1012 GAATGC GAATGC(1/-1)
BspEI 2754 TCCGGA T^CCGGA
BspLU11I 1157 ACATGT A^CATGT
Bsu36I 477 CCTTAGG CC^T[ACGT]AGG
CelII 1332 GCTGAGC GC^T[ACGT]AGC
Eam1105I 664 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 477 CCTTAGG CC^T[ACGT]AGG
HincII 589 GTCAAC GT[CT]^[GA]AC
HindII 589 GTCAAC GT[CT]^[GA]AC
MamI 1290 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2272 ACGCGT A^CGCGT
MluNI 23 TGGCCA TGG^CCA
MroI 2754 TCCGGA T^CCGGA
MscI 23 TGGCCA TGG^CCA
NcoI 2532 CCATGG C^CATGG
NotI 194 GCGGCCGC GC^GGCCGC
PvuI 120 CGATCG CGAT^CG
SfiI 2304 GGCCGCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2473 CACCGGCG C[GA]^CCGG[CT]G
SphI 2452 GCATGC GCATG^C
StuI 1460 AGGCCT AGG^CCT
TfiI 1149 GATTC G^A[AT]TC
Tsp509I 471 AATT ^AATT
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 1157 ACATGT A^C[GA][CT]GT
2272 ACGCGT
AlwNI 1270 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2584 CAGGCCCTG
Aor51HI 784 AGCGCT AGC^GCT
1235 AGCGCT
AspHI 539 GTGCAC G[AT]GC[AT]^C
727 GTGCTC
AspI 1893 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1962 GACGCCGTC
BbrPI 580 CACGTG CAC^GTG
978 CACGTG
BbvCI 2160 CCTCAGC CCTCAGC(-5/-2)
1536 GCTGAGG
BsaI 2655 GAGACC GGTCTC(1/5)
2703 GGTCTC
BsiHKAI 539 GTGCAC G[AT]GC[AT]^C
727 GTGCTC
BsrBI 1979 CCGCTC CCGCTC(-3/-3)
1702 GAGCGG
BssHII 2080 GCGCGC G^CGCGC
2082 GCGCGC
BstYI 275 AGATCC [GA]^GATC[CT]
1489 GGATCC
EarI 835 CTCTTC CTCTTC(1/4)
2125 CTCTTC
Eco47III 784 AGCGCT AGC^GCT
1235 AGCGCT
EcoNI 1002 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1692 CCTGCAGAAGG
HphI 2095 GGTGA GGTGA(8/7)
2723 TCACC
Ksp632I 835 CTCTTC CTCTTC(1/4)
2125 CTCTTC
MflI 275 AGATCC [GA]^GATC[CT]
1489 GGATCC
NspI 1157 ACATGT [GA]CATG^[CT]
2452 GCATGC
PflFI 1893 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1962 GACGCCGTC
PflMI 26 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2485 CCATCGGATGG
PleI 1554 GACTC GAGTC(4/5)
1111 GAGTC
PmaCI 580 CACGTG CAC^GTG
978 CACGTG
PmlI 580 CACGTG CAC^GTG
978 CACGTG
Tth111I 1893 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1962 GACGCCGTC
Van91I 26 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2485 CCATCGGATGG
XhoII 275 AGATCC [GA]^GATC[CT]
1489 GGATCC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PpuMI
PstI PvuII SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XmaCI XmaI